Establishing PMT Adjustment Tolerances Protocol

Dilute blank and dyed beads at 1 drop of each in 1.6 mL of lab use diluent and mix via vortex or tapping.Set the PMT voltages for all fluorescence channels of interest to those used for daily QC to ensure that all peaks are able to be seen.Place the...

Daily Monitoring to Assess Instrument Performance

For a daily quality check, Quantum QC can be used to measure fluorescence at optimal PMT settings to ensure that the flow cytometer is performing its best. These PMT settings should be held consistent to gauge instrument performance day-to-day, and...

Daily Cytometer Setup using Quantum QC.

Setting PMTs to Ensure Consistent Fluorescence MeasurementsQuantum QC can be used to set up PMTs for measuring consistent fluorescence intensity day to day. One, or several, of the fluorescent peaks can be monitored daily and PMT levels adusted to...

I’m looking to do fluorescence quantitation using your Quantum MESF kits. One concern I had however was that my unlabeled cells have a lower fluorescence intensity than the blank bead. Why is that, and what do I do in this instance?

Our Quantum MESF and Quantum Simply Cellular beads are composed of polystyrene that have some level of autofluorescence varying based on the laser/channel in use. If you have control cells with lower MFI (mean fluorescence intensity) values than our...

See how our MESF & QSC kits support quantitative flow cytometry in cancer research & immunotherapy development.

Enjoy this curated listing of references related to cancer research & immunotherapy development.Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist....

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Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links.  If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!
 
 
 
 
 
 
 

For more information, see our flow tutorial page or our entire library in Tech Support