Employing Nucleic Acid Conjugated Microspheres in the Field of Flow Cytometry: Part III

In part 1 of our series we discussed the significance of employing nucleic acid conjugated beads in the field of Flow Cytometry. In section II we discussed the optimal concentration of nucleic acid to generate a conjugate suitable for flow cytometry...

Employing Nucleic Acid Conjugated Microspheres in the Field of Flow Cytometry: Part II

Last week we discussed how nucleic acid attachment to microspheres for subsequent flow cytometry analysis has become an important technique in biotechnology research and development. Beads and attached ligands are utilized by both researchers and...

Employing Nucleic Acid Conjugated Microspheres in the Field of Flow Cytometry: Part I

Analysis of nucleic acids using flow cytometry has become a more frequent practice. Horejsh et al. developed a fluid array system using microsphere-conjugated molecular beacons and flow cytometry for the specific, multiplexed detection of unlabeled...

Flow Cytometer as a QC Tool for Assessing Bead Coating Efficacy

Attaching ligands to bead surfaces is a valuable technique for the development of a variety of assays and flow applications. While there are several means of ligand attachment, covalent coupling is often employed for the immobilization of...

Fluorescent Microspheres: Internal vs. External Labeling

Continuing our discussion of the different types of microspheres that can be advantageous for flow cytometry, we think it is useful to now discuss the different ways beads may be labeled with fluorophore and the typical characteristics that...

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Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links.  If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!
 
 
 
 
 
 
 

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