Extracellular vesicle analysis by flow cytometry

When studying extracellular vesicles, it is helpful to have some tools to guide the operator in their analysis. Particle reference standardsassist the investigator by providing confidence in sizing, quantitation and particle count. In continuing...

Advanced Instrumentation Improves Microparticle Analysis

Exosome research is getting hotter, so we are going to periodically include articles regarding the topic. Small particle analysis requires some extra steps to resolve a particle. There are new technologies and modifications to standard flow...

Evaluating Cell Sorting Parameters

Cell sorting is a powerful tool that allows a user to isolate a single cell from a culture sample. Though this is a powerful technique, errors in the sorting process can oftentimes cause a scientist much grief in both time and money. No one wants to...

Why are dim peaks usually so wide? Does this mean the dye distribution is more heterogenous within these beads?

Wider dim peaks are partially due to an instrumental artifact called the photoelectron statistical error. Essentially this is counting/conversion error in the PMT (the instrument detects x amount of photons for which it’s supposed to create y amount...

Establishing PMT Adjustment Tolerances Protocol

Dilute blank and dyed beads at 1 drop of each in 1.6 mL of lab use diluent and mix via vortex or tapping.Set the PMT voltages for all fluorescence channels of interest to those used for daily QC to ensure that all peaks are able to be seen.Place the...

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Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links.  If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!
 
 
 
 
 
 
 

For more information, see our flow tutorial page or our entire library in Tech Support