What are you using to measure results in your flow cytometric study?

See how the Quantum MESF system has been used to standardize studies and quantify results in a range of applications:Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, et al. (2013) Manufacture of Clinical-Grade CD19-Specific T Cells Stably...

I’m using your Quantum Simply Cellular (QSC) beads to quantitate cell surface receptors, but I’m fixing my cells as part of my standard prep. Do I also need to fix the beads as well?

Sample handling/prep can certainly play a large role in quantitative flow cytometry, although we recommend against using fixation steps for our beads, as many of the fixatives are known solvents of our polystyrene particles (e.g. formaldehyde or...

Flow Cytometry’s Top Ten Contributions to the World compiled from musings of the Purdue Cytometry Discussion List

Purdue Cytometry Discussion List

I ran some beads and noticed there are two or three populations. I thought the beads were only a single size? What’s going on?

What this user is noticing is likely aggregation of the beads. FSC/SSC (forward scatter / side scatter) dot plots provide general information in terms of size (FSC) and granularity (SSC). When two particles are stuck together, the instrument will...

Do you have tools for assessing receptor occupancy (RO) in biopharmaceutical development?

Flow cytometry is an important technology for conducting receptor occupancy assays in drug / biotherapeutic development, including free receptor, total receptor, bound receptor and receptor modulation formats. Our Quantum MESF and Quantum Simply...


Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links.  If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!

For more information, see our flow tutorial page or our entire library in Tech Support