TECHNICAL SUPPORT DOCUMENTS

LS001 Magnetic Separator Flux Density
TSD 0026 Centrifugation Chart
TSD 0030: Buffers
TSD 0706: Handling & Pipetting Concentration Standards
TSD 726: Microsphere Decontamination
Protocols for different levels and methods of decontamination, including irradiation, pasteurization, and ethanol sterilization.
So You Want To Work With Beads? Tips for beginners
5 Bead Blunders

TECHNOTES

201: Working with Microspheres 
Choosing, cleaning, characterizing, coating beads, etc. (04/08)

201A: Microsphere Selection
Description of properties that impact base bead selection. (01/17)

202: Microsphere Aggregation
Preventing, detecting, and reversing aggregation. Chemicals and equipment sources. (04/09)

203: Washing Microspheres
Protocols for different methods for cleaning microspheres; advantages/disadvantages of methods; suppliers of equipment. (03/17)

203A: Drying & Re-Suspending Microspheres
Converting aqueous suspensions to dry microspheres and vice versa. (06/13)

204: Adsorption to Microspheres
Adsorbing proteins onto particles; use of "surface diluents" (blockers); recipes and references. (04/08)

205: Covalent Coupling
Chemical attachment of proteins, nucleic acids, etc. to functionalized microspheres. Suggestions for coupling protocol design and optimization (buffer and blocker selection, use of chemical linkers, etc.). Recommended reading and references. (04/08)

206: Equations
For calculating particles/mL, surface area/g, "parking area," settling velocity @ 1G and in centrifuge, etc. (10/12)

208: Microsphere Sizing
BLI methods are described and discussed, along with Optical Microscopy and other methods. (08/09)