Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications we stock an extensive inventory of fluorescent microspheres and offer custom dyeing services.
Europium chelate nanoparticles
Our highly-fluorescent europium (III) nanoparticles offer exceptional stability, in addition to well-functionalized carboxylated surfaces for the covalent attachment of ligand. Our Eu (III) nanoparticles have been used to develop highly sensitive assays (microplate, lateral flow) based on time-resolved (TRF) fluorescence and as particulate labels in chemiluminence assays. The beads are supplied as ~1% solids (w/v) aqueous suspensions. View excitation & emission spectra and Instrument Links for TRF strip test readers.
Catalog Number | Description | Nominal Diameter | Specification Range | Product Data Sheet |
---|---|---|---|---|
FCEU001 | Europium Chelate PS - COOH | 0.10µm | 0.090 - 0.110µm | PDS 741.pdf |
FCEU002 | Europium Chelate PS - COOH | 0.20µm | 0.190 - 0.210µm | PDS 741.pdf |
FCEU003 | Europium Chelate PS - COOH | 0.30µm | 0.270 - 0.330µm | PDS 741.pdf |
FCEU004 | Europium Chelate PS - COOH | 0.40µm | 0.370 - 0.430µm | PDS 741.pdf |
references
Liang R-L, Xu X-P, Liu T-C, Zhou J-W, Wang X-G, Ren Z-Q, Hao F, Wu Y-S. (2015) Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips. Anal Chim Acta; 891:277-283.
Soukka T, Paukkunen J, Härmä H, Lönnberg S, Lindroos H, Lövgren. (2001) Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem; 47(4):(1269-1278
Xia X, Xu Y, Ke R, Zhang H, Zou M, Yang W, Li Q. (2013) A highly sensitive nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue. Anal Bioanal Chem; 405(23):7541-4.
Yeo SJ, Bao DT, Seo GE, Bui CT, Kim DTH, Anh NTV, … Park H. (2017). Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles. Scientific Reports, 7, 7933.
Fluorescent Polymer
Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Unless noted otherwise, fluorescent polymer microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.
Catalog Number | Fluorescence Color | Nominal Diameter | Specification Range |
---|---|---|---|
FSDG001 | Dragon Green | 0.05µm | 0.040 - 0.060µm |
FSFR001 | Flash Red | 0.05µm | 0.040 - 0.060µm |
FSDG002 | Dragon Green | 0.20µm | 0.190 - 0.210µm |
FSSY002 | Suncoast Yellow | 0.20µm | 0.190 - 0.210µm |
FSFR002 | Flash Red | 0.20µm | 0.190 - 0.210µm |
FSDG003 | Dragon Green | 0.50µm | 0.470 - 0.530µm |
FSPP003 | Plum Purple | 0.50µm | 0.470 - 0.530µm |
FSFR003 | Flash Red | 0.50µm | 0.470 - 0.530µm |
FSDG004 | Dragon Green | 1.00µm | 0.95 - 1.05µm |
FSEG004 | Envy Green | 1.00µm | 0.95 - 1.05µm |
FSPP004 | Plum Purple | 1.00µm | 0.95 - 1.05µm |
FSFR004 | Flash Red | 1.00µm | 0.95 - 1.05µm |
FSDG005 | Dragon Green | 2.00µm | 1.80 - 2.20µm |
FSPP005 | Plum Purple | 2.00µm | 1.80 - 2.20µm |
FSFR005 | Flash Red | 2.00µm | 1.80 - 2.20µm |
FSDG006 | Dragon Green | 4.00µm | 3.80 - 4.20µm |
FSEG006 | Envy Green | 4.00µm | 3.80 - 4.20µm |
FSFR006 | Flash Red | 4.00µm | 3.80 - 4.20µm |
FSDG007 | Dragon Green | 7.5µm | 7.30 - 7.70µm |
FSSY007 | Suncoast Yellow | 7.5µm | 7.30 - 7.70µm |
FSFR007 | Flash Red | 7.5µm | 7.30 - 7.70µm |
FSEG008 | Envy Green | 10.0µm | 9.50 - 10.50µm |
FSDG009 | Dragon Green | 15.00µm | 14.5 - 15.50µm |
FSDG011 | Dragon Green | >25.00µm | >25.00µm |
References
Lee, M, Reece, P, Marchington, R, Metzger, N, Dholakia, K (2007) Construction and calibration of an optical trap on a fluorescence optical microscope. Nat. Protocols V.2 p3226-3238
Mattheyses, A, Simon, S, Rappoport, J (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist J Cell Sci 123: 3621-3628
Sun, Z, Martinez-Lemus, L, Trache, A, Trzeciakowski, J, Davis, G, Pohl, U, Meininger,G (2005) Mechanical properties of the interaction between fibronectin and α5β1-integrin on vascular smooth muscle cells studied using atomic force microscopy. Am J Physiol Heart Circ Physiol, 289(6) H2526-H2535
Ogilvie, J, Beaurepaire, E, Alexandrou, A, Joffre, M, (2006) Fourier-transform coherent anti-Stokes Raman scattering microscopy. Optics Letters, Optical Society of America, 31(4), p480-2.
Fluorescent Carboxyl Polymer
Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.
Catalog Number | Fluorescence Color | Nominal Diameter | Specification Range |
---|---|---|---|
FCDG001 | Dragon Green | 0.05µm | 0.040 - 0.060µm |
FCFR001 | Flash Red | 0.05µm | 0.040 - 0.060µm |
FCDG002 | Dragon Green | 0.10µm | 0.090 - 0.110µm |
FCDG003 | Dragon Green | 0.20µm | 0.190 - 0.210µm |
FCSG003 |
Surf Green |
0.20µm | 0.190 - 0.210µm |
FCFR003 | Flash Red | 0.20µm | 0.190 - 0.210µm |
FCGB003 | Glacial Blue | 0.20µm | 0.190 - 0.210µm |
FCDG004 | Dragon Green | 0.40µm | 0.370 -0.430µm |
FCFR004 | Flash Red | 0.40µm | 0.370 -0.430µm |
FCDG005 | Dragon Green | 0.50µm | 0.470 - 0.530µm |
FCFR005 | Flash Red | 0.50µm | 0.470 - 0.530µm |
FCDG006 | Dragon Green | 1.0µm | 0.95 - 1.05µm |
FCEG006 | Envy Green | 1.0µm | 0.95 - 1.05µm |
FCSY006 | Suncoast Yellow | 1.0µm | 0.95 - 1.05µm |
FCGB006 | Glacial Blue | 1.0µm | 0.95 - 1.05µm |
FCFR006 | Flash Red | 1.0µm | 0.95 - 1.05µm |
FCSY007 | Suncoast Yellow | 2.0µm | 1.80 - 2.20µm |
FCDG008 | Dragon Green | 5.0µm | 4.80 - 5.20µm |
FCEG008 | Envy Green | 5.0µm | 4.80 - 5.20µm |
FCGB008 | Glacial Blue | 5.0µm | 4.80 - 5.20µm |
FCFR008 | Flash Red | 5.0µm | 4.80 - 5.20µm |
FCDG009 | Dragon Green | 10.0µm | 9.50 - 10.50µm |
Fluorescent Magnetic Polymer
Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the polymer matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.
Catalog Number | Description | Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet | Safety Data Sheet |
---|---|---|---|---|---|---|
MCDG001 | Classical Magnetic Polymer | Dragon Green | 0.9µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MCFR001 | Classical Magnetic Polymer | Flash Red | 0.9µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEFR001 | Encapsulated Magnetic Polymer | Flash Red | .86µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEDG002 | Encapsulated Magnetic Polymer | Dragon Green | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MESY002 | Encapsulated Magnetic Polymer | Suncoast Yellow | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEGB002 | Encapsulated Magnetic Polymer | Glacial Blue | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEFR002 | Encapsulated Magnetic Polymer | Flash Red | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
UMGB001 | COMPEL™ Magnetic COOH modified | Glacial Blue | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMDG001 | COMPEL™ Magnetic COOH modified | Dragon Green | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMEG001 | COMPEL™ Magnetic COOH modified | Envy Green | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMFR001 | COMPEL™ Magnetic COOH modified | Flash Red | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMGB002 | COMPEL™ Magnetic COOH modified | Glacial Blue | 6µm | 5.50 - 6.50µm | PDS 705.pdf | SDS MF221.pdf |
UMDG002 | COMPEL™ Magnetic COOH modified | Dragon Green | 6µm | 5.50 - 6.50µm | PDS 705.pdf | SDS MF221.pdf |
UMFR002 | COMPEL™ Magnetic COOH modified | Flash Red | 6µm | 5.50 - 6.50µm | PDS 705.pdf | SDS MF221.pdf |
UMGB003 | COMPEL™ Magnetic COOH modified | Glacial Blue | 8µm | 7.50 - 8.50µm | PDS 705.pdf | SDS MF221.pdf |
UMDG003 | COMPEL™ Magnetic COOH modified | Dragon Green | 8µm | 7.50 - 8.50µm | PDS 705.pdf | SDS MF221.pdf |
UMFR003 | COMPEL™ Magnetic COOH modified | Flash Red | 8µm | 7.50 - 8.50µm | PDS 705.pdf | SDS MF221.pdf |
References
Ebert, SN, Taylor, DG, Nguyen, HL, Kodack, DP, Beyers, RJ, Xu, Y, Yang, Z, French, BA (2007) Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques. STEM CELLS 25:2936–2944.
Trost, A, Motloch, K, Bruckner, D, Schroedl, F, Bognera, B, Kaser-Eichberger, A, Runge, … Reitsamer, H.A.(2015) Time-dependent retinal ganglion cell loss, microglial activation and blood-retina-barrier tightness in an acute model of ocular hypertension. Exp Eye Res V136, p59–71
Fluorescent Protein Coated Polymer
Our inventory includes a variety of streptavidin coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions. Please select the catalog code link below or contact our Customer Service Department for specific availability.
Catalog Number | Coating / Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet |
---|---|---|---|---|
CFDG001 | SA / Dragon Green | 0.20µm | 0.190 - 0.210µm | PDS 721.pdf |
CFDG002 | SA / Dragon Green | 0.40µm | 0.370 - 0.430µm | PDS 721.pdf |
CFFR002 | SA / Flash Red | 0.40µm | 0.370 - 0.430µm | PDS 721.pdf |
CFDG003 | SA / Dragon Green | 0.50µm | 0.470 - 0.530µm | PDS 721.pdf |
CFDG004 | SA / Dragon Green | 1.00µm | 0.95 - 1.05µm | PDS 721.pdf |
CFFR004 | SA / Flash Red | 1.00µm | 0.95 - 1.05µm | PDS 721.pdf |