Microspheres are routinely coated with ligands such as antibodies, oligonucleotides and peptides for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and Glutaraldehyde are two of the most common crosslinkers used in bead coating protocols. To purchase as a kit, see our coupling kits.
EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres.
Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.
PDS 740, Accessory Reagents
SDS AR 400, (EDAC)
SDS AR 401, (Glutaraldehyde)
Catalog Number | Description | Volume | Product Data Sheet | Safety Data Sheet |
---|---|---|---|---|
AA010-1 | DEPC - Carbodiimide (EDAC) | 1g | PDS 740.pdf | SDS AR400.pdf |
AA010-5 | DEPC - Carbodiimide (EDAC) | 5g | PDS 740.pdf | SDS AR400.pdf |
AA012 | Glutaraldehyde, EM Grade, 25% | 10 × 10mL (ampoules) | PDS 740.pdf | SDS AR401.pdf |