Flow Cytometry Blog

Flow Cytometry Blog

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Employing Nucleic Acid Conjugated Microspheres in the Field of Flow Cytometry: Part I

Analysis of nucleic acids using flow cytometry has become a more frequent practice. Horejsh et al. developed a fluid array system using microsphere-conjugated molecular beacons and flow cytometry for the specific, multiplexed detection of unlabeled...

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Flow Cytometer as a QC Tool for Assessing Bead Coating Efficacy

Attaching ligands to bead surfaces is a valuable technique for the development of a variety of assays and flow applications. While there are several means of ligand attachment, covalent coupling is often employed for the immobilization of...

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Fluorescent Microspheres: Internal vs. External Labeling

Continuing our discussion of the different types of microspheres that can be advantageous for flow cytometry, we think it is useful to now discuss the different ways beads may be labeled with fluorophore and the typical characteristics that...

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Why We Don't Offer Dyed Silica

In the previous blog, Refractive Index and Small Particle Analysis, we discussed some of the reasons why people want to employ silica nanoparticles as calibrators when analyzing extracellular vesicles on the flow cytometer. So, why don’t we...

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Refractive Index and Small Particle Analysis

We often receive requests for silica rather than polystyrene size standards for flow cytometric studies, notably for the analysis of extracellular vesicles. This is because the refractive index (RI) of silica (~1.44) is closer to that of cells (~1....

Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links. If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!