Affinity Ligand Coated Microspheres
Affinity binding ligands can aid in the rapid development or streamlined production of microsphere reagents. They also provide the opportunity for optimal orientation of the ligand, as with end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.
Affinity ligands are also used extensively in isolation and purifications. Streptavidin-coated microspheres are often used to capture biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for immunoprecipitation and the purification of antibodies and Fc-tagged fusion proteins. Lectin-coated spheres (e.g. WGA, ConA) are used to bind glycoproteins, glycolipids and other glycan-presenting targets.
Streptavidin
Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® magnetic particle offerings.
TechNote 101, Affinity Ligand Coated Microspheres
PDS 721, Streptavidin Coated Microspheres
Streptavidin Polystyrene
| Catalog Number | Nominal Diameter | Specification Range |
|---|---|---|
| CP01000 | 0.10µm | 0.090 - 0.110µm |
| CP01001 | 0.20µm | 0.190 - 0.210µm |
| CP01003 | 0.50µm | 0.470 - 0.530µm |
| CP01004 | 1.00µm | 0.95 - 1.05µm |
| CP01005 | 3.0µm | 2.80 - 3.20µm |
| CP01006 | 5.00µm | 4.80 - 5.20µm |
| CP01007 | 10.00µm | 9.50 - 10.50µm |
| CP01008 | 15.0µm | 14.50 - 15.50µm |
References
Nandin IS, Fong C, Deantonio C, Torreno-Pina J, Pecetta S, Maldonado P, … Batista, F.(2017) Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies. J Exp Med.20170633. (0.11µm Streptavidin-coated microspheres)
Wu SJ,Chan A, Kado CI. (2004) Detection of PCR amplicons from bacterial pathogens using microsphere agglutination J Microbiol Methods Vol 56, Issue 3, 395-400 (5.7µm Streptavidin-coated microspheres)
Walter S, HerrgenL, Schoor O, Jung G, Wernet D, Bühring HJ, … Stevanović S.(2003) Cutting Edge: Predetermined Avidity of Human CD8 T Cells Expanded on Calibrated MHC/Anti-CD28-Coated Microspheres. J Immunol. 171(10)4974-4978 (5.6µm Streptavidin as artificial-antigen presenting cells [aAPC])
Jeffers, Faye, et al. "Mucin–lectin interactions assessed by flow cytometry." Carbohydrate research 345.10 (2010): 1486-1491.(3.09µm Streptavidin PS & 2.01µm Silica)
Streptavidin Magnetic Polymer
The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Streptavidin magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See also our BioMag® magnetic particle offerings.
TechNote 101, Affinity Ligand Coated Microspheres
PDS 721, Streptavidin Coated Microspheres
| Catalog Number | Description | Nominal Diameter | Specification Range |
|---|---|---|---|
| UMC0100 | SA / COMPEL | 3µm | 2.50 - 3.50µm |
| UMC0101 | SA / COMPEL | 6µm | 5.50 - 6.50µm |
| UMC0102 | SA / COMPEL | 8µm | 7.50 - 8.50µm |
References
Pitsillides, CM, Joe, EK, Wei, X, Anderson, RR, & Lin, CP. (2003). Selective cell targeting with light-absorbing microparticles and nanoparticles. Biophysical journal, 84(6), 4023-4032.(0.83µm Streptavidin magnetic)
PDS 756, Magnefy™ Microspheres
PDS 715, ProMag® Microspheres
PDS 743, ProMag®HP Microspheres
PDS 749, Magnetic Sampler Packs
| Catalog Number | Description | Product Data Sheet | Safety Data Sheet |
|---|---|---|---|
| MFYS1N | Magnefy™ • SA 1µm | PDS 756.pdf | SDS MY100.pdf |
| PMS1N | ProMag™ 1 Series • Streptavidin | PDS 715.pdf | SDS CG154.pdf |
| PMS3HP | ProMag™ HP 3 Series • Streptavidin | PDS 743.pdf | SDS CG154.pdf |
| PMS3N | ProMag™ 3 Series • Streptavidin | PDS 715.pdf | SDS CG154.pdf |
| 21950 | Streptavidin Magnetic Sample Pack MFYS1N, Magnefy 1µm SA - 1mL (1% solids) PMS1N, ProMag 1 Series SA - 1mL (1% solids) PMS3HP, ProMag HP 3μm SA - 1mL (1% solids) BP628, BioMagPlus SA - 2mL (5mg / mL) |
PDS 749.pdf |
References
Linz TH, Henley WH, & Ramsey JM. (2017) Photobleaching kinetics-based bead encoding for multiplexed bioassays. Lab on a Chip, 17(6), 1076-1082. (3µm ProMag Streptavidin with immobilized b-PE and b-Qdot labels)
BioMag® Streptavidin / Biotin
PDS 551, BioMag® Streptavidin
PDS 621, BioMag®Plus Streptavidin and Kit
PDS 530, BioMag® Streptavidin, Nuclease-free
SDS MB232
| Catalog Number | Description | Concentration (mg/mL) | Volume (mL) | Product Data Sheet | Safety Data Sheet |
|---|---|---|---|---|---|
| BM551 | BioMag® Streptavidin | 5 | 5, 50 | PDS 551.pdf | SDS MB232.pdf |
| BP628 | BioMag®Plus Streptavidin | 5 | 10 | PDS 621.pdf | SDS MB232.pdf |
| BM568 | BioMag® Streptavidin Nuclease-free | 1 | 10, 25 | PDS 530.pdf | SDS MB232.pdf |
| BP621 | BioMag®Plus Streptavidin/Biotin Binding Kit | 5 | 5 | PDS 621.pdf |
References
Chang D, Tram K, Li B, Feng Q, Shen Z, Lee CH, ... & Li, Y. (2017). Detection of DNA Amplicons of Polymerase Chain Reaction Using Litmus Test. Scientific reports, 7(1), 3110.(Cat. BM551BioMag Streptavidin used in colorimetric detection of C. difficile)
Streptavidin Fluorescent Polystyrene
Streptavidin-coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions.
PDS 721, Steptavidin-coated Microspheres
PDS 731, Fluorescent Microspheres
Spectra of Fluorescent Dyes
| Catalog Number | Fluorescence Color | Nominal Diameter | Specification Range |
|---|---|---|---|
| CFDG001 | SA / Dragon Green | 0.20µm | 0.190 - 0.210µm |
| CFDG002 | SA / Dragon Green | 0.40µm | 0.370 - 0.430µm |
| CFFR002 | SA / Flash Red | 0.40µm | 0.370 - 0.430µm |
| CFDG003 | SA / Dragon Green | 0.50µm | 0.470 - 0.530µm |
| CFDG004 | SA / Dragon Green | 1.00µm | 0.95 - 1.05µm |
| CFFR004 | SA / Flash Red | 1.00µm | 0.95 - 1.05µm |
References
Bhattacharyya, K., Goldschmidt, B. S., & Viator, J. A. (2016). Detection and capture of breast cancer cells with photoacoustic flow cytometry. Journal of biomedical optics, 21(8), 087007. (320nm streptavidin-coated Flash Red fluorescent nanoparticles + biotin-EpCAM)
Streptavidin Dyed Polystyrene
Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.
PDS 721, Steptavidin-coated Microspheres
PDS 717, Visibly Dyed Microspheres Color Palette
| Catalog Number | Coating / Dye | Nominal Diameter | Specification Range |
|---|---|---|---|
| CDCR001 | SA / Crimson Red | 0.20µm | 0.190 - 0.210µm |
| CDCB001 | SA / Cabo Blue | 0.20µm | 0.190 - 0.210µm |
References
Wang Y, Yan W, Fu S, Hu S, Wang Y, Xu J, & Ye C. (2018). Multiple cross displacement amplification coupled with nanoparticles-based lateral flow biosensor for detection of Staphylococcus aureus and identification of methicillin-resistant S. aureus. Frontiers in Microbiology, 9.(0.129µm Streptavidin-coated Crimson Red nanoparticles)
Streptavidin Silica
SiO2 with a streptavidin-coated surface for the attachment of biomolecules. Silica streptavidin microspheres are sold in these standard amounts: 1mL, 2mL, 5mL, and 10mL. Available at 1% solids (w/v), in three nominal mean diameters: ~0.5µm, ~1.0µm, and ~5.0µm.
PDS 721, Streptavidin-coated Microspheres
PDS 702, Silica Microspheres
SDS SG142
| Catalog Number | Nominal Diameter | Specification Range |
|---|---|---|
| CS01000 | 0.5µm | 0.460 - 0.540µm |
| CS01001 | 1.0µm | 0.95 - 1.05µm |
| CS01002 | 5.00µm | 4.80 - 5.20µm |
References
Blumberg, S, Gajraj, A, Pennington, M, Meiners, J (2005) Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy. Biophysical Journal V89(2)p1272–1281
Wang J, Xu D, Kawde A, Polsky R (2001) Metal Nanoparticle-Based Electrochemical Stripping Potentiometric Detection of DNA Hybridization Anal Chem. 73(22), 5576-5581
Biotin
Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See also our BioMag® offerings.
TechNote101, Affinity Ligand-coated Microspheres
PDS 724, Biotin-coated Microspheres
| Catalog Number | Description | Nominal Diameter | Specification Range |
|---|---|---|---|
| CP10000 | Polymeric / Biotin | 10µm | 9.50 - 10.50µm |
Protein A and Protein G
Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® offerings.
TechNote101, Affinity Ligand-coated Microspheres
TSD 0025, Protein A&G Antibody Affinities
PDS 722, Protein A Coated Microspheres
| Catalog Number | Description | Nominal Diameter | Specification Range |
|---|---|---|---|
| CP02000 | Polymer / Protein A | 1.00µm | 0.95-1.05µm |
| CP02001 | Polymer / Protein A | 5.00µm | 4.80-5.20µm |
| CP02002 | Polymer / Protein A | 10.0µm | 9.50-10.50µm |
| PMG3N | ProMag™ (Magnetic) / Protein G | 3µm | 2.70 - 3.30µm |
References
Reynolds NM, Mohammadalipour A, Hall CR, Adib AA, Farnoud AM, Burdick MM. (2018) Galectin-1 Influences Breast Cancer Cell Adhesion to E-selectin Via Ligand Intermediaries. Cell Mol Bioeng; 11(1):37-52. Note: (Protein A / E-selectin [hFc] beads used to investigate E-selectin / Gal-1 mediated adhesion in breast cancer monolayers)
BioMag® Secondary Ab
BioMag® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles.
| Catalog Number | Description | Concentration (mg / mL) | Volume (mL) | Product Data Sheet | Safety Data Sheet |
|---|---|---|---|---|---|
| BM563 | BioMag® Goat anti-Human IgG | 1 | 50 | PDS 563.pdf | SDS MB234.pdf |
| BM562 | BioMag® Goat anti-Human IgG (Fc Specific) | 5 | 50 | PDS 562.pdf | SDS MB233.pdf |
| BM561 | BioMag® Goat anti-Human IgM | 1 | 50 | PDS 561.pdf | SDS MB235.pdf |
| BM549 | BioMag® Goat anti-Mouse IgG | 1 | 50 & 500 | PDS 549.pdf | SDS MB234.pdf |
| BM550 | BioMag® Goat anti-Mouse IgG (Fc Specific) | 1 | 50 & 500 | PDS 550.pdf | SDS MB233.pdf |
| BP619 | BioMag®Plus Goat anti-Mouse IgG | 1 | 50 | PDS 619.pdf | SDS MB234.pdf |
| BP612 | BioMag®Plus Goat anti-Mouse IgG Antibody Coupling Kit | 1 | 25 | PDS 619.pdf | |
| BM558 | BioMag® Goat anti-Mouse IgM | 1 | 50 & 500 | PDS 558.pdf | SDS MB234.pdf |
| BM559 | BioMag® Goat anti-Rabbit IgG | 1 | 50 & 500 | PDS 559.pdf | SDS MB234.pdf |
| BM560 | BioMag® Goat anti-Rat IgG | 1 | 50 & 500 | PDS 560.pdf | SDS MB234.pdf |
| BM548 | BioMag® Goat anti-Rat IgG (Fc specific) | 5 | 50 & 500 | PDS 548.pdf | SDS MB233.pdf |
BioMag® Lectin
Our ConA- and WGA coated BioMag®Plus microparticles provide a convenient means for isolating glycoproteins from serum or cell lysate, and for investigating other lectin/glycan-mediated processes. The BioMag®Plus magnetic particle format provides high surface area, and permits easy and efficient separations.
PDS 716, BioMag®Plus Wheat Germ Agglutinin
PDS 720, BioMag®Plus Concanavalin A
TSD 0028, Concanavalin A (Con A) Cut&Run
SDS MB233 (Cat. BP530)
SDS MB237 (Cat. BP531)
| Catalog Number | Description | Concentration (mg/mL) | Volume (mL) |
|---|---|---|---|
| BP530 | BioMag®Plus Wheat Germ Agglutinin | 5 | 3, 10 |
| BP531 | BioMag® Plus Concanavalin A | 5 | 3, 10 |