Oh, how we love to accessorize!
Bangs Laboratories offers a variety of accessory products for use in microparticle reagent development. The collection includes crosslinking reagents and surfactants, as well as solutions and buffers for microsphere coating and storage. These products complement our extensive catalog of polymer, silica, and magnetic microparticles, and also offer a convenient means to replenish individual components of coupling kits.
Buffers and Solutions
Bangs Bead Coupling Buffers are ready-to-use buffers that are available in a variety of pH levels (4.5 to 9.0). They can be used as coupling or wash buffers for plain, dyed, or functionalized polymer microspheres. Our Bangs Bead Storage Buffer is available in two pH levels: 7.4 and 8.5.
Our Bead Solution is a ready-to-use aqueous suspending solution for the dilution and/or storage of your uncoated plain, dyed, or functionalized polymer microspheres (both carboxylated or aminated). An antimicrobial agent deters microbial contamination, and stabilizers promote suspension dispersity, peace of mind, and harmonious accord in the laboratory.
|Catalog Code||Description||pH||Volume (mL)||Product Data Sheet||Material Safety Data Sheet|
|BUFF1||Bangs Bead Coupling Buffer||4.5||250, 500, 1000, 2000||PDS 738.pdf||SDS AR405.pdf|
|BUFF2||Bangs Bead Coupling Buffer||6.0||250, 500, 1000, 2000||PDS 738.pdf||SDS AR406.pdf|
|BUFF3||Bangs Bead Coupling Buffer||7.4||250, 500, 1000, 2000||PDS 738.pdf||SDS AR407.pdf|
|BUFF4||Bangs Bead Coupling Buffer||9.0||250, 500, 1000, 2000||PDS 738.pdf||SDS AR408.pdf|
|BUFF5||Bangs Bead Storage Buffer||7.4||250, 500, 1000, 2000||PDS 738.pdf||SDS AR409.pdf|
|BUFF6||Bangs Bead Storage Buffer||8.5||250, 500, 1000, 2000||PDS 738.pdf||SDS AR410.pdf|
|SOLN1||Bangs Bead Solution||-||500, 1000, 2000||PDS 738.pdf||SDS AR411.pdf|
Microspheres are routinely coated with ligands such as antibodies, oligonucleotides, and peptides, for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and glutaraldehyde are two of the most common crosslinkers used in bead coating protocols.
EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres, while Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.
|Catalog Code||Description||Volume||Product Data Sheet||Material Safety Data Sheet|
|AA010||DEPC - Carbodiimide (EDAC)||1g & 5g||PDS 740.pdf||SDS AR400.pdf|
|AA012||Glutaraldehyde, EM Grade, 25%||10 × 10 mL (ampoules)||PDS 740.pdf||SDS AR401.pdf|
Surfactants are commonly utilized with microspheres composed of hydrophobic polymer matrices such as polystyrene to facilitate bead wetting and to deter or treat aggregation. Common concentrations are ~0.1% for uncoated polymer beads and ~0.01% in the storage buffer of coated polymer, magnetic, and silica beads.
Triton® X-100 and Tween® 20 are nonionic surfactants used in the storage buffers of coated bead suspensions, while Sodium Dodecyl Sulfate (SDS) is an anionic surfactant that decreases hydrophobicity and participates in charge stabilization of the suspension.
|Catalog Code||Description||Volume (g)||Product Data Sheet||Material Safety Data Sheet|
|AA014||Triton™ X-100 Nonionic Surfactant||10||PDS 739.pdf||SDS AR402.pdf|
|AA016||Tween® 20 Nonionic Surfactant||10||PDS 739.pdf||SDS AR403.pdf|
|AA018||Sodiom Dodecyl Sulfate Anionic Surfactant||10||PDS 739.pdf||SDS AR404.pdf|