We manufacture a number of standards intended for use with either image-based cell viability analyzers (e.g. trypan blue, Vi-CELL®) or flow cytometers (DAPI, propidium iodide, LIVE/DEAD® stains, etc) that are important to bioprocessing and research programs.

ViaCheck™ Viability and Concentration Standards

Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Viability Controls are supplied at ~1.0 x 106 beads / mL and are available in a range of live/dead ratios. ViaCheck™ Concentration Controls are supplied at the noted concentrations. 


PDS 706, ViaCheck™ Viability Controls
PDS 711, ViaCheck™ Concentration Controls
TSD 0706, Handling & Pipetting Concentration Standards
Appl.Note 0708, Optimization of Vi-CELL® Settings for Calibration checks using ViaCheck™ Controls
TSD 0711, ViaCheck™ for Cell Viability Analyzers: Best Practices
Video: ViaCheck™ Protocol
TSD 0712, Repeatability of Coulter Z2 and Vi-CELL Counts using ViaCheck™ 0.50e+6 Concentration Controls
SDS ST352, (ViaCheck™ Viability)
SDS ST353, (ViaCheck™ Concentration)

Catalog Number Description Volume (mL)
VC10B ViaCheck™ 0% Viability Control 20
VC25B ViaCheck™ 25% Viability Control 20
VC20B ViaCheck™ 50% Viability Control 20
VC30B ViaCheck™ 75% Viability Control 20
VC40B ViaCheck™ 90% Viability Control 20
VC50B ViaCheck™ 100% Viability Control 20
VC50N ViaCheck™ Concentration Control (0.5 × 106) 20
VC60N ViaCheck™ Concentration Control (1 × 106) 20
VC70N ViaCheck™  Concentration Control (4 × 106) 20
VC80N ViaCheck™  Concentration Control (8 × 106) 20

 ViaCheck™ Viability and Concentration Standards - SingleShots™

ViaCheck™ SingleShots™, affordable single-use QC. Mix, dispense & run for confirmation of live/dead ratios and counts. ViaCheck™ SingleShots™ minimize error and maximize efficiency by circumventing potential human error from repeated bottle resuspension & pipetting steps. 



PDS 706, ViaCheck™ Viability Controls
PDS 711, ViaCheck™ Concentration Controls
TSD 0706, Handling & Pipetting Concentration Standards
Appl.Note 0708, Optimization of Vi-CELL® Settings for Calibration checks using ViaCheck™ Controls
TSD 0711, ViaCheck™ for Cell Viability Analyzers: Best Practices
Video: ViaCheck™ Protocol
TSD 0712, Repeatability of Coulter Z2 and Vi-CELL Counts using ViaCheck™ 0.50e+6 Concentration Controls
SDS ST352, (ViaCheck™ Viability)
SDS ST353, (ViaCheck™ Concentration)

Catalog Number Description Units
VC10BSS ViaCheck™ 0% Viability Control SingleShots™ 25 or 75
VC25BSS ViaCheck™ 25% Viability Control SingleShots™ 25 or 75
VC20BSS ViaCheck™ 50% Viability Control SingleShots™ 25 or 75
VC30BSS ViaCheck™ 75% Viability Control SingleShots™ 25 or 75
VC40BSS ViaCheck™ 90% Viability Control SingleShots™ 25 or 75
VC50BSS ViaCheck™ 100% Viability Control SingleShots™ 25 or 75
VC50NSS ViaCheck™ Concentration Control (0.5 × 106) SingleShots™ 25 or 75
VC60NSS ViaCheck™ Concentration Control (1 × 106) SingleShots™ 25 or 75
VC70NSS ViaCheck™ % Concentration Control (4 × 106) SingleShots™ 25 or 75
VC80NSS ViaCheck™ % Concentration Control (8 × 106) SingleShots™ 25 or 75

References

Kaminski RW, Wu M, Turbyfill  KR, Clarkson K, Tai B, Bourgeois AL, Van De Verg LL, Walker RI, Oaks EV. (2014) Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine. Clin Vaccine Immunol; 21(3):366-382.

Cadena-Herrera D, Esparza-De Lara JE, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E. (2015) Validation of three viable-cell counting methods: manual, semi-automated, and automated. Biotechnol Reports; 7:9-16.

Lew C, Gomez J, Rhyner M (2012) Instrument-to-instrument Variability in the Vi-CELL Automated Viability Analyzer. Beckman Coulter Life Sciences

Chen A, Leith M, Tu R, Tahim G, Sudra A, Bhargava S. (2017) Effects of diluents on cell culture viability measured by automated cell counter. PLoS One; 12(3):e0173375.

Pagliuca F W, Millman JR, Gürtler M, Segel M, Van Dervort A, Ryu JH, ... & Melton DA. (2014). Generation of functional human pancreatic β cells in vitro. Cell, 159(2), 428-439.

Fluorescence Reference Standards - FLOW

We offer microspheres labeled with traditional dyes/stains that may be used as standards to support flow cytometric assays in cell cycle analysis or cell viability determinations. Beads may be used for routine instrument set-up and QC, or as test-specific standards. See also our full range of Fluorescence Reference Standards .

PDS 890, Fluorescence Reference Standards
Brochure: Cell Cycle Analysis
SDS ST331, (Fluorescence Reference Standards)
SDS ST334, (Fluorescence Reference Standards)

Catalog Number Description
897 Acridine Orange
906 DAPI
891 Fluorescein (FITC)
894 Hoechst 33342
892 Propidium Iodide

Viability Dye Compensation Standard - Flow

Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain. Supplied at ~1e+7 beads/mL.


PDS 853, Viability Dye Compensation Standard
SDS ST340, (VIability Dye Compensation Standard)

Catalog Number Description
450 Viability Dye Compensation Standard, 4µm
451 Viability Dye Compensation Standard, 8µm

References

Estes, Myka L., et al. "Identification of endothelial cells and progenitor cell subsets in human peripheral blood." Current protocols in cytometry (2010): 9-33.