Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications, Bangs stocks an extensive array of fluorescent microspheres.

Fluorescence intensity standards

Fluorescence intensity standards have many applications both in instrument QC programs and in biological analyses. We offer two intensity standards for these applications. Both the Dragon Green and Flash Red Intensity Kits consist of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of their respective fluorophore.

The different intensity populations may serve as relative intensity standards for applications in fluorescence microscopy, where, as internally-dyed beads, they will stand up to the rigors of imaging. The beads may also serve as bright relative intensity or linearity standards for flow cytometry; ask about our QuickCal® linearity template if this is your interest.

Dragon Green Intensity Standard

The Dragon Green Intensity Standard is a kit consisting of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets.

Catalog Number Description Product Data Sheet Safety Data Sheet
DG06M Dragon Green Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Flash Red Intensity Standard

The Flash Red Intensity Standard is a kit consisting of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard.

Catalog Number Description Product Data Sheet Safety Data Sheet
FR06M Flash Red Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Fluorescent Polymer

Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Number Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
FSDG001 Dragon Green 0.05µm 0.040 - 0.060µm PDS 731.pdf
FSFR001 Flash Red 0.05µm 0.040 - 0.060µm PDS 731.pdf
FSDG002 Dragon Green 0.20µm 0.190 - 0.210µm PDS 731.pdf
FSSY002 Suncoast Yellow 0.20µm 0.190 - 0.210µm PDS 731.pdf
FSFR002 Flash Red 0.20µm 0.190 - 0.210µm PDS 731.pdf
FSDG003 Dragon Green 0.50µm 0.470 - 0.530µm PDS 731.pdf
FSPP003 Plum Purple 0.50µm 0.470 - 0.530µm PDS 731.pdf
FSFR003 Flash Red 0.50µm 0.470 - 0.530µm PDS 731.pdf
FSDG004 Dragon Green 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSEG004 Envy Green 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSPP004 Plum Purple 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSFR004 Flash Red 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSDG005 Dragon Green 2.00µm 1.80 - 2.20µm PDS 731.pdf
FSPP005 Plum Purple 2.00µm 1.80 - 2.20µm PDS 731.pdf
FSFR005 Flash Red 2.00µm 1.80 - 2.20µm PDS 731.pdf
FSDG006 Dragon Green 4.00µm 3.80 - 4.20µm PDS 731.pdf
FSEG006 Envy Green 4.00µm 3.80 - 4.20µm PDS 731.pdf
FSFR006 Flash Red 4.00µm 3.80 - 4.20µm PDS 731.pdf
FSDG007 Dragon Green 7.5µm 7.30 - 7.70µm PDS 731.pdf
FSSY007 Suncoast Yellow 7.5µm 7.30 - 7.70µm PDS 731.pdf
FSFR007 Flash Red 7.5µm 7.30 - 7.70µm PDS 731.pdf
FSEG008 Envy Green 10.0µm 9.50 - 10.50µm PDS 731.pdf
FSDG009 Dragon Green 15.00µm 14.5 - 15.50µm PDS 731.pdf
FSDG011 Dragon Green >25.00µm >25.00µm PDS 731.pdf

Fluorescent Carboxyl Polymer

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. See spectra for available fluorescent dyes. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Number Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
FCDG001 Dragon Green 0.05µm 0.040 - 0.060µm PDS 731.pdf
FCFR001 Flash Red 0.05µm 0.040 - 0.060µm PDS 731.pdf
FCDG002 Dragon Green 0.10µm 0.090 - 0.110µm PDS 731.pdf
FCDG003 Dragon Green 0.20µm 0.190 - 0.210µm PDS 731.pdf
FCSG003

Surf Green

0.20µm 0.190 - 0.210µm PDS 731.pdf
FCFR003 Flash Red 0.20µm 0.190 - 0.210µm PDS 731.pdf
FCGB003 Glacial Blue 0.20µm 0.190 - 0.210µm PDS 731.pdf
FCDG004 Dragon Green 0.40µm 0.370 -0.430µm PDS 731.pdf
FCFR004 Flash Red 0.40µm 0.370 -0.430µm PDS 731.pdf
FCDG005 Dragon Green 0.50µm 0.470 - 0.530µm PDS 731.pdf
FCFR005 Flash Red 0.50µm 0.470 - 0.530µm PDS 731.pdf
FCDG006 Dragon Green 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCEG006 Envy Green 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCSY006 Suncoast Yellow 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCGB006 Glacial Blue 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCFR006 Flash Red 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCSY007 Suncoast Yellow 2.0µm 1.80 - 2.20µm PDS 731.pdf
FCDG008 Dragon Green 5.0µm 4.80 - 5.20µm PDS 731.pdf
FCEG008 Envy Green 5.0µm 4.80 - 5.20µm PDS 731.pdf
FCGB008 Glacial Blue 5.0µm 4.80 - 5.20µm PDS 731.pdf
FCFR008 Flash Red    5.0µm 4.80 - 5.20µm PDS 731.pdf
FCDG009 Dragon Green 10.0µm 9.50 - 10.50µm PDS 731.pdf
FCDG011 Dragon Green 15µm 14.50 - 15.50µm PDS 731.pdf

Fluorescent Magnetic Polymer

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the bead matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres are highly uniform and spherical. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Number Description Fluorescence Color Nominal Diameter Specification Range Product Data Sheet Safety Data Sheet
MCDG001 Classical Magnetic Polymer Dragon Green 0.9µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MCFR001 Classical Magnetic Polymer Flash Red 0.9µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEDG001 Encapsulated Magnetic Polymer Dragon Green .86µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEFR001 Encapsulated Magnetic Polymer Flash Red .86µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEDG002 Encapsulated Magnetic Polymer Dragon Green 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MESY002 Encapsulated Magnetic Polymer Suncoast Yellow 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEGB002 Encapsulated Magnetic Polymer Glacial Blue 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEFR002 Encapsulated Magnetic Polymer Flash Red 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
UMGB001 COMPEL™ Magnetic COOH modified Glacial Blue 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMDG001 COMPEL™ Magnetic COOH modified Dragon Green 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMEG001 COMPEL™ Magnetic COOH modified Envy Green 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMFR001 COMPEL™ Magnetic COOH modified Flash Red 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMGB002 COMPEL™ Magnetic COOH modified Glacial Blue 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMDG002 COMPEL™ Magnetic COOH modified Dragon Green 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMFR002 COMPEL™ Magnetic COOH modified Flash Red 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMGB003 COMPEL™ Magnetic COOH modified Glacial Blue 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf
UMDG003 COMPEL™ Magnetic COOH modified Dragon Green 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf
UMFR003 COMPEL™ Magnetic COOH modified Flash Red 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf

Fluorescent Protein Coated Polymer

Our inventory includes a variety of streptavidin coated fluorescent microspheres. Please select the catalog code link below or contact our Customer Service Department for specific availability.

Catalog Number Coating / Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
CFDG001 SA / Dragon Green 0.20µm 0.190 - 0.210µm PDS 721.pdf
CFDG002 SA / Dragon Green 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFFR002 SA / Flash Red 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFDG003 SA / Dragon Green 0.50µm 0.470 - 0.530µm PDS 721.pdf
CFDG004 SA / Dragon Green 1.00µm 0.95 - 1.05µm PDS 721.pdf
CFFR004 SA / Flash Red 1.00µm 0.95 - 1.05µm PDS 721.pdf