SiO2 with natural hydroxyl or silanol (SiOH) surface groups for adsorption of nucleotides with low protein binding (see TechNote 104). Non-functionalized silica microspheres are available as ~10% solids (w/w) aqueous suspensions or in the dry form in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.

Silica in aqueous suspension

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Nominal Diameter Specification Range Product Data Sheet Safety Data Sheet
SS02000 0.15µm 0.120 - 0.180µm PDS 702.pdf SDS SG140.pdf
SS02001 0.30µm 0.260 - 0.340µm PDS 702.pdf SDS SG140.pdf
SS02002 0.40µm 0.360 - 0.440µm PDS 702.pdf SDS SG140.pdf
SS03000 0.50µm 0.460 - 0.540µm PDS 702.pdf SDS SG140.pdf
SS03001 0.70µm 0.660 - 0.740µm PDS 702.pdf SDS SG140.pdf
SS03002 0.90µm 0.860 - 0.940µm PDS 702.pdf SDS SG140.pdf
SS04000 1.0µm 0.95 - 1.05µm PDS 702.pdf SDS SG140.pdf
SS04001 1.50µm 1.40 - 1.60µm PDS 702.pdf SDS SG140.pdf
SS04002 2.0µm 1.80 - 2.20µm PDS 702.pdf SDS SG140.pdf
SS05000 2.50µm 2.30 - 2.70µm PDS 702.pdf SDS SG140.pdf
SS05001 3.0µm 2.80 - 3.20µm PDS 702.pdf SDS SG140.pdf
SS05002 4.0µm 3.8 - 4.20µm PDS 702.pdf SDS SG140.pdf
SS05003 5.0µm 4.80 - 5.20µm PDS 702.pdf SDS SG140.pdf
SS06000 >5.0µm >5.0µm PDS 702.pdf SDS SG140.pdf

Dry Silica 

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Nominal Diameter Specification Range Product Data Sheet Safety Data Sheet
SSD2001 0.30µm 0.260 - 0.340µm PDS 702.pdf SDS SG141.pdf
SSD3000 0.50µm 0.460 - 0.540µm PDS 702.pdf SDS SG141.pdf
SSD4000 1.0µm 0.95 - 1.05µm PDS 702.pdf SDS SG141.pdf
SSD4001 1.50µm 1.40 - 1.60µm PDS 702.pdf SDS SG141.pdf
SSD5000 2.50µm 2.30 - 2.70µm PDS 702.pdf SDS SG141.pdf
SSD5001 3.0µm 2.80 - 3.20µm PDS 702.pdf SDS SG141.pdf
SSD5002 4.0µm 3.80 - 4.20µm PDS 702.pdf SDS SG141.pdf
SSD5003 5.0µm 4.80 - 5.20µm PDS 702.pdf SDS SG141.pdf

References

Ferguson JA, Steemers FJ, Walt DR. (2000) High-density fiber-optic DNA random microsphere array. Anal Chem; 72(22):5618-5624.

Lauer S, Goldstein B, Nolan RL, Nolan JP. (2002) Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry; 41(6):1742-51.

Steinberg G, Stromsborg K, Thomas L, Barker D, Zhao C. (2004) Strategies for covalent attachment of DNA to beads. Biopolymers; 73(5):597-605.

B Sharma, P Bugga, L Madison, A Henry, M Blaber, N Greeneltch, ... R. Van Duyne (2016) Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman Spectroscopy  Am Chem Soc, 138 (42), pp 13952–13959

G Steinberg-Tatman,M Huynh, D Barker, C Zhao (2006) Synthetic Modification of Silica Beads That Allows for Sequential Attachment of Two Different Oligonucleotides Bioconjugate Chem 17(3), 841-848

N Clack, K Salaita, J Groves (2008) Electrostatic readout of DNA microarrays with charged microspheres. Nat Biotech 26,825-830

Lu Q, Liu H, Cao T (2013) Efficient Isolation of Bone Marrow Adipocyte Progenitors by Silica Microbeads Incubation. Stem Cells Dev. V22:18

Khan Z, Poetter K, Park DJ.(2008) Enhanced solid phase PCR: mechanisms to increase priming by solid support primers. Anal Biochem. Apr 15;375(2):391-3.

Marrec P, Doglioli M, Grégori G, Dugenne M, Della Penna A, Bhairy N, … Thyssen M (2017) Coupling physics and biogeochemistry thanks to high resolution observations of the phytoplankton community structure in the North-Western Mediterranean Sea Biogeosciences Discuss, https://doi.org/10.5194/bg-2017-343 (0.4µm - 7µm silica microspheres as FSC standards for phytoplankton on the CytoBuoy CytoSense)

Wang, Z. (2016). Microsphere super-resolution imaging. Nanoscience, 3, 193-210. (4.7µm silica microspheres)

Crites, T. J., Maddox, M., Padhan, K., Muller, J., Eigsti, C., & Varma, R. (2015). Supported lipid bilayer technology for the study of cellular interfaces. Current protocols in cell biology, 24-5. (lipid bilayers [aAPCs, artificial antigen-presenting cells] on 5µm silica microspheres)

Zanoni, M., Habimana, O., Amadio, J., & Casey, E. (2016). Antifouling activity of enzyme‐functionalized silica nanobeads. Biotechnology and bioengineering, 113(3), 501-512.(500nm silica microspheres, functionalized & coated with Proteinase K for biofilm / biofouling control)

Tolić-Nørrelykke, SF, Schäffer, E, Howard, J, Pavone, FS, Jülicher, F., & Flyvbjerg, H. (2006) Calibration of optical tweezers with positional detection in the back focal plane. Review of scientific instruments, 77(10), 103101.(SS04001, 1.54µm silica microspheres; PS03001, 0.523µm polystyrene microspheres)

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