Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications we stock an extensive inventory of fluorescent microspheres and offer custom dyeing services. See spectra for available fluorescent dyes.

Europium chelate nanoparticles

Our highly-fluorescent europium (III) nanoparticles offer exceptional stability, in addition to well-functionalized carboxylated surfaces for the covalent attachment of ligand. Our Eu (III) nanoparticles have been used to develop highly sensitive assays (microplate, lateral flow) based on time-resolved (TRF) fluorescence and as particulate labels in chemiluminence assays. The beads are supplied as ~1% solids (w/v) aqueous suspensions. View excitation & emission spectra and Instrument Links for TRF strip test readers. See how we stack up against our competition in our Europium Chelate brochure.

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
FCEU001 Europium Chelate PS - COOH 0.10µm 0.090 - 0.110µm PDS 741.pdf
FCEU002 Europium Chelate PS - COOH 0.20µm 0.190 - 0.210µm PDS 741.pdf
FCEU003 Europium Chelate PS - COOH 0.30µm 0.270 - 0.330µm PDS 741.pdf
Catalog Number Description Product Data Sheet
21960 Europium Chelate COOH Sampler Pack (1mL of 0.10μm, 0.20μm, 0.30μm) PDS 741.pdf

references

Liang R-L, Xu X-P, Liu T-C, Zhou J-W, Wang X-G, Ren Z-Q, Hao F, Wu Y-S. (2015) Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips. Anal Chim Acta; 891:277-283.

Soukka T, Paukkunen J, Härmä H, Lönnberg S, Lindroos H, Lövgren. (2001) Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem; 47(4):(1269-1278

Xia X, Xu Y, Ke R, Zhang H, Zou M, Yang W, Li Q. (2013) A highly sensitive nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue. Anal Bioanal Chem; 405(23):7541-4.

Yeo SJ, Bao DT, Seo GE, Bui CT, Kim DTH, Anh NTV, … Park H. (2017). Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles. Scientific Reports, 7, 7933.

Tuan Bao D, Kim DTH, Park H, Cuc BT, Ngoc NM, Linh NTP, … Yeo SJ. (2017) Rapid Detection of Avian Influenza Virus by Fluorescent Diagnostic Assay using an Epitope-Derived Peptide Theranostics Vol 7, Issue 7 1835-1846

Tang Y, Zhang H, Liu X, Trofimchuk E, Feng S, Ma T, …, Lu X. (2017) Advantage of Eu3+ -Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk. J Food Sci. Mar;82(3):694-697 (200nm Eu chelate microspheres)

Do Thi Hoang Kim DT, Bao HP, & Nguyen Minh Ngoc, SJY. (2018). Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine. Theranostics, 8(13), 3629.(FCEU001, 0.1µm Euroipium chelate COOH nanoparticles)

Fluorescent Polystyrene

Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Unless noted otherwise, fluorescent polymer microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
FSDG001 Dragon Green 0.05µm 0.040 - 0.060µm PDS 731.pdf
FSFR001 Flash Red 0.05µm 0.040 - 0.060µm PDS 731.pdf
FSDG002 Dragon Green 0.20µm 0.190 - 0.210µm PDS 731.pdf
FSSY002 Suncoast Yellow 0.20µm 0.190 - 0.210µm PDS 731.pdf
FSFR002 Flash Red 0.20µm 0.190 - 0.210µm PDS 731.pdf
FSDG003 Dragon Green 0.50µm 0.470 - 0.530µm PDS 731.pdf
FSPP003 Plum Purple 0.50µm 0.470 - 0.530µm PDS 731.pdf
FSFR003 Flash Red 0.50µm 0.470 - 0.530µm PDS 731.pdf
FSDG004 Dragon Green 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSEG004 Envy Green 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSPP004 Plum Purple 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSFR004 Flash Red 1.00µm 0.95 - 1.05µm PDS 731.pdf
FSDG005 Dragon Green 2.00µm 1.80 - 2.20µm PDS 731.pdf
FSPP005 Plum Purple 2.00µm 1.80 - 2.20µm PDS 731.pdf
FSFR005 Flash Red 2.00µm 1.80 - 2.20µm PDS 731.pdf
FSDG006 Dragon Green 4.00µm 3.80 - 4.20µm PDS 731.pdf
FSEG006 Envy Green 4.00µm 3.80 - 4.20µm PDS 731.pdf
FSFR006 Flash Red 4.00µm 3.80 - 4.20µm PDS 731.pdf
FSDG007 Dragon Green 7.5µm 7.30 - 7.70µm PDS 731.pdf
FSSY007 Suncoast Yellow 7.5µm 7.30 - 7.70µm PDS 731.pdf
FSFR007 Flash Red 7.5µm 7.30 - 7.70µm PDS 731.pdf
FSEG008 Envy Green 10.0µm 9.50 - 10.50µm PDS 731.pdf
FSDG009 Dragon Green 15.00µm 14.5 - 15.50µm PDS 731.pdf
FSDG011 Dragon Green >25.00µm >25.00µm PDS 731.pdf

References

Lee, M, Reece, P, Marchington, R, Metzger, N, Dholakia, K (2007) Construction and calibration of an optical trap on a fluorescence optical microscope. Nat. Protocols V.2 p3226-3238

Mattheyses, A, Simon, S, Rappoport, J (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist J Cell Sci 123: 3621-3628

Sun, Z, Martinez-Lemus, L, Trache, A, Trzeciakowski, J, Davis, G, Pohl, U, Meininger,G (2005) Mechanical properties of the interaction between fibronectin and α5β1-integrin on vascular smooth muscle cells studied using atomic force microscopy. Am J Physiol Heart Circ Physiol, 289(6) H2526-H2535

Ogilvie, J, Beaurepaire, E, Alexandrou, A, Joffre, M, (2006) Fourier-transform coherent anti-Stokes Raman scattering microscopy. Optics Letters, Optical Society of America, 31(4), p480-2.

Chen X, Wang J, Versluis M, de Jong N, Villanueva FS.(2013) Ultra-fast bright field and fluorescence imaging of the dynamics of micrometer-sized objects. Review of Scientific Instruments 84,063701

Fluorescent Carboxyl Polystyrene

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes. See also our Quantumplex™ kits for suspension array development.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
FCDG001 Dragon Green 0.05µm 0.040 - 0.060µm PDS 731.pdf
FCFR001 Flash Red 0.05µm 0.040 - 0.060µm PDS 731.pdf
FCDG002 Dragon Green 0.10µm 0.090 - 0.110µm PDS 731.pdf
FCDG003 Dragon Green 0.20µm 0.190 - 0.210µm PDS 731.pdf
FCSG003

Surf Green

0.20µm 0.190 - 0.210µm PDS 731.pdf
FCFR003 Flash Red 0.20µm 0.190 - 0.210µm PDS 731.pdf
FCGB003 Glacial Blue 0.20µm 0.190 - 0.210µm PDS 731.pdf
FCDG004 Dragon Green 0.40µm 0.370 -0.430µm PDS 731.pdf
FCFR004 Flash Red 0.40µm 0.370 -0.430µm PDS 731.pdf
FCDG005 Dragon Green 0.50µm 0.470 - 0.530µm PDS 731.pdf
FCFR005 Flash Red 0.50µm 0.470 - 0.530µm PDS 731.pdf
FCDG006 Dragon Green 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCEG006 Envy Green 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCSY006 Suncoast Yellow 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCGB006 Glacial Blue 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCFR006 Flash Red 1.0µm 0.95 - 1.05µm PDS 731.pdf
FCSY007 Suncoast Yellow 2.0µm 1.80 - 2.20µm PDS 731.pdf
FCDG008 Dragon Green 5.0µm 4.80 - 5.20µm PDS 731.pdf
FCEG008 Envy Green 5.0µm 4.80 - 5.20µm PDS 731.pdf
FCGB008 Glacial Blue 5.0µm 4.80 - 5.20µm PDS 731.pdf
FCFR008 Flash Red    5.0µm 4.80 - 5.20µm PDS 731.pdf
FCDG009 Dragon Green 10.0µm 9.50 - 10.50µm PDS 731.pdf
FCDG011 Dragon Green 15µm 14.50 - 15.50µm PDS 731.pdf

References

Adams EW, Ueberfeld J, Ratner DM, O'Keefe BR, Walt DR, Seeberger PH.(2003) Encoded fiber-optic microsphere arrays for probing protein-carbohydrate interactions.Angew Chem Int Ed Engl.10;42(43):5317-20. (Cat. 235, QuantumPlex Starfire Red 4.4µm COOH)

Shapiro, K, Mazet, JA, Schriewer, A, Wuertz, S, Fritz, H, Miller, WA, … & Conrad, PA. (2010) Detection of Toxoplasma gondii oocysts and surrogate microspheres in water using ultrafiltration and capsule filtration. Water Research, 44(3), 893-903. (10.35µm Dragon Green Carboxyl, 8.6µm Glacial Blue Carboxyl)

Pei X, Yin H, Lai T, Zhang J, Liu F, Xu X, Li N. (2017) Multiplexed Detection of Attomoles of Nucleic Acids Using Fluorescent Nanoparticle Counting Platform Anal. Chem.10.1021/acs.analchem.7b04551 (~200nm carboxylated Glacial Blue, Dragon Green & Envy Green beads)

Fluorescent Magnetic Polymer

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the polymer matrix. For specific information on the use of fluorescent magnetic particles for cell labeling & tracking, see TSD 0748. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Description Fluorescence Color Nominal Diameter Specification Range Product Data Sheet Safety Data Sheet
MCDG001 Classical Magnetic Polymer Dragon Green 0.9µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MCFR001 Classical Magnetic Polymer Flash Red 0.9µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEDG001 Encapsulated Magnetic Polymer Dragon Green .86µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEFR001 Encapsulated Magnetic Polymer Flash Red .86µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEDG002 Encapsulated Magnetic Polymer Dragon Green 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MESY002 Encapsulated Magnetic Polymer Suncoast Yellow 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEGB002 Encapsulated Magnetic Polymer Glacial Blue 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEFR002 Encapsulated Magnetic Polymer Flash Red 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
UMGB001 COMPEL™ Magnetic COOH modified Glacial Blue 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMDG001 COMPEL™ Magnetic COOH modified Dragon Green 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMEG001 COMPEL™ Magnetic COOH modified Envy Green 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMFR001 COMPEL™ Magnetic COOH modified Flash Red 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMGB002 COMPEL™ Magnetic COOH modified Glacial Blue 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMDG002 COMPEL™ Magnetic COOH modified Dragon Green 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMFR002 COMPEL™ Magnetic COOH modified Flash Red 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMGB003 COMPEL™ Magnetic COOH modified Glacial Blue 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf
UMDG003 COMPEL™ Magnetic COOH modified Dragon Green 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf
UMFR003 COMPEL™ Magnetic COOH modified Flash Red 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf

References

Hinds, K, Hill, J, Shapiro, E, Laukkanen, M, Alfonso C. Silva, Combs,…Dunbar, C (2003) Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. Blood Aug 102 (3) 867-872

Shapiro, E M, Skrtic, S, Koretsky, AP (2005) Sizing it up: Cellular MRI using micron-sized iron oxide particles. Magn. Reson. Med., 53: 329–338

Rohani, R, de Chickera, SN, Willert, C, Chen, Y, Dekaban, G, Foster, P (2011) In Vivo Cellular MRI of Dendritic Cell Migration Using Micrometer-Sized Iron Oxide (MPIO) Particles Mol Imaging Biol 13: 679

Slotkin,J, Cahill, K, Tharin, S, Shapiro, EM (2207) Cellular Magnetic Resonance Imaging: Nanometer and Micrometer Size Particles for Noninvasive Cell Localization Neurotherapeutics, V4,3,P428-433

Tarulli E, Chaudhuri JD, Gretka V, Hoyles A, Morshead CM, Stanisz GJ. (2013) Effectiveness of micron-sized superparamagnetic iron oxide particles as markers for detection of migration of bone marrow-derived mesenchymal stromal cells in a stroke model. J Magn Reson Imaging.Jun;37(6):1409-18. (MCDG001, 0.9µm Dragon Green Magnetic Classical, 60% mag)

Fluorescent Protein Coated Polystyrene

Our inventory includes a variety of streptavidin coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions. Please select the catalog code link below or contact our Customer Service Department for specific availability. See spectra for available fluorescent dyes.

Catalog Number Coating / Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
CFDG001 SA / Dragon Green 0.20µm 0.190 - 0.210µm PDS 721.pdf
CFDG002 SA / Dragon Green 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFFR002 SA / Flash Red 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFDG003 SA / Dragon Green 0.50µm 0.470 - 0.530µm PDS 721.pdf
CFDG004 SA / Dragon Green 1.00µm 0.95 - 1.05µm PDS 721.pdf
CFFR004 SA / Flash Red 1.00µm 0.95 - 1.05µm PDS 721.pdf

StarLight™ Calibration Slides

Our StarLight™ Calibration Slides feature vibrant ~6µm fluorescent microspheres dyed with a single fluorophore for basic imaging checks and calibration. Our four standard versions are appropriate for use with common microscope filter sets: Glacial Blue (360, 450), Dragon Green (480, 520), Envy Green (525, 565), and Flash Red (660, 690). The calibration slides are available individually or as the full 4-slide collection. See fluorophore spectra here.
Catalog Number Description Product Data Sheet
SL1GB StarLight™ Calibration Slide - Glacial Blue PDS 857.pdf
SL1DG StarLight™ Calibration Slide - Dragon Green PDS 857.pdf
SL1EG StarLight™ Calibration Slide - Envy Green PDS 857.pdf
SL1FR StarLight™ Calibration Slide - Flash Red PDS 857.pdf
SL04K StarLight™ Collection - Slide 4 -Pack PDS 857.pdf

Dragon Green Intensity Standard

The Dragon Green Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets. View excitation and emission spectra.

Catalog Number Description Product Data Sheet Safety Data Sheet
DG06M Dragon Green Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Flash Red Intensity Standard

The Flash Red Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard. View excitation and emission spectra.

Catalog Number Description Product Data Sheet Safety Data Sheet
FR06M Flash Red Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Flow Cytometry

See also our extensive Flow Cytometry products.