Though particle counting technology applications are diverse, there is a common need for instrument validation and ongoing QC. Microsphere-based particle count standards may be used to validate liquid counters across their dynamic ranges and to ensure continued capability through the performance of daily QC checks. The use of a reference material permits the standardization of results between runs, instruments and laboratories, and over time. Our particle count standards include both general and instrument-focused (flow cytometer, cell viability analyzer) controls.

SureCount™ Particle Count Standard

SureCount™ standards are suspensions of polymer microspheres intended for the validation and monitoring of particle counters and supporting sample preparation processes. The diameters of the SureCount™ standards are traceable to NIST Standard Reference Materials. The standards are supplied as ~1 x 106 microspheres/mL aqueous suspensions in 10mL volumes, and each bottle is provided with a Certificate of Traceability. For information on proper mixing and sampling, see TSD 0706: Handling & Pipetting Concentration Standards.

Catalog Number Product Data Sheet Nominal Mean Diameter (µm) Volume (mL) Safety Data Sheet
CC03N PDS 733.pdf 3 10 SDS ST350.pdf
CC05N PDS 733.pdf 5 10 SDS ST350.pdf
CC10N PDS 733.pdf 10 10 SDS ST350.pdf
CC15N PDS 733.pdf 15 10 SDS ST350.pdf

References

Kim J, Kim E, Bae S, Kwon S, Chun H.(2013) Potentiometric Multichannel Cytometer Microchip for High-throughput Microdispersion Analysis Anal. Chem.85 (1), pp 362–368

Garzio LM, Steinberg DK, Erickson M, & Ducklow HW. (2013). Microzooplankton grazing along the Western Antarctic Peninsula. Aquatic Microbial Ecology, 70(3), 215-232.(Cat. CC10N, SureCount 10µm)

Bekeschus S, Moritz J, Schmidt A, & Wende K. (2017). Redox regulation of leukocyte-derived microparticle release and protein content in response to cold physical plasma-derived oxidants. Clinical Plasma Medicine, 7, 24-35.

Flow Cytometry Absolute Count Standard™


 
Our Flow Cytometry Absolute Count Standard™ is a precisely counted population of microspheres for estimating counts of unlabeled cells via flow cytometry. The microspheres are ~7-9µm in diameter and supplied at ~1e+6 beads/mL. Beads are internally labeled with multiple fluorophores for excitation with common lasers (e.g. 488nm, 633nm) and discrimination from the cell population. By evaluating the ratio of microspheres to cells, the volumetric number of cells may be determined.
Catalog Number Nominal Mean Diameter (µm) Product Data Sheet Safety Data Sheet
580 Flow Cytometry Absolute Count Standard™ PDS 880.pdf SDS ST334.pdf

References

Heinrich LF, Andersen DK, Cleasby ME, & Lawson C. (2015). Long-term high fat feeding of rats results in increased numbers of circulating microvesicles with pro-inflammatory effects on endothelial cells. British Journal of Nutrition, 113(11), 1704-1711.

ViaCheck™  Concentration Controls

Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Concentration Controls are supplied at the noted concentrations. For information on proper mixing and sampling, see TSD 0706: Handling & Pipetting Concentration Standards. or to request a copy of Optimization of Vi-CELL® XR Settings for Calibration Checks Using ViaCheck™ Controls & ViaCheck™ for Cell Viability Analyzers: Best Practices, click here.

Catalog Number Description Volume (mL) Product Data Sheet Safety Data Sheet
VC50N ViaCheck™ Concentration Control (0.5 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC60N ViaCheck™ Concentration Control (1 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC70N ViaCheck™ % Concentration Control (4 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC80N ViaCheck™ % Concentration Control (8 × 106) 20 PDS 711.pdf SDS ST353.pdf

 ViaCheck™  Concentration Controls - SingleShots™

ViaCheck™ SingleShots™, affordable single-use QC. Mix, dispense & run for confirmation of live/dead ratios and counts. ViaCheck™ SingleShots™ minimize error and maximize efficiency by circumventing potential human error from repeated bottle resuspension & pipetting steps. To request a copy of Optimization of Vi-CELL® XR Settings for Calibration Checks Using ViaCheck™ Controls & ViaCheck™ for Cell Viability Analyzers: Best Practices, click here.

Catalog Number Description Units Product Data Sheet Safety Data Sheet
VC50NSS ViaCheck™ Concentration Control (0.5 × 106) SingleShots™ 25 or 75 PDS 711.pdf SDS ST353.pdf
VC60NSS ViaCheck™ Concentration Control (1 × 106) SingleShots™ 25 or 75 PDS 711.pdf SDS ST353.pdf
VC70NSS ViaCheck™ % Concentration Control (4 × 106) SingleShots™ 25 or 75 PDS 711.pdf SDS ST353.pdf
VC80NSS ViaCheck™ % Concentration Control (8 × 106) SingleShots™ 25 or 75 PDS 711.pdf SDS ST353.pdf