Trypan Blue

Trypan blue exclusion is a common method for the determination of cell viability. It is used extensively in biopharmaceutical cell culture programs, and for a range of research studies including apoptosis, cytopathic effects of viral infection, and effects of sample processing methods on cell viability and concentration. Our solution is suitable for traditional manual staining and hemocytometer counting. NOTE: This product is not intended to be a plug-in replacement bottle for automated cell viability analyzers. Instrument manufacturers please contact us for bulk/OEM packaging if this is your interest.

 special oFfer!

We are so excited about cell viability determinations that we are offering a free bottle of Trypan Blue when you purchase ViaCheck Viability or ViaCheck Concentration. Place an order for any ViaCheck product. Use code word: BLUE in the comments section of your online order form or when ordering by phone, and we’ll send you a 125mL bottle of Trypan blue FREE!

Catalog Number Description Volume (mL) Product Data Sheet Safety Data Sheet
AA020 Trypan Blue 0.4% Solution 125mL PDS 748.pdf SDS AR414.pdf
AA021 Trypan Blue 0.4% Solution 250mL PDS 748.pdf SDS AR414.pdf


Louis KS, Siegel AC. (2011) Cell viability analysis using trypan blue: manual and automated methods. Methods Mol Biol; 740:7-12.

Phelan K, May KM.(2017) Mammalian Cell Tissue Culture Techniques. Curr Protoc Mol Biol.5;117:A.3F.1-A.3F.23