Options for Quantifying Flow Cytometry Fluorescence

When it comes to fluorescence quantification in flow cytometry, Bangs Labs provides two options for your consideration, Quantum™ MESF and Quantum™ Simply Cellular®. We recommend selecting one system and using it for the duration of the project.  Our Quantum™ MESF and Quantum™ Simply Cellular® beads are external standards that enable the standardization of fluorescence intensity units irrespective of cytometer and software. Because they are labeled with the same fluorochromes used to label cells, they provide a synchronous response to the environment. General characteristics for each system are summarized below. For more detail, please see the associated Product Data Sheet (PDS) or watch our related videos.

Quantification System 1: MESF

  • Externally labeled with fluorochromes
  • Divide final values by F:P ratio to attain final result (expression level)
  • Come prelabeled with the following fluorescent options: FITC, Alexa Fluor® 488, PE, Alexa Fluor® 647, PE-Cy™5, or R-PE APC
  • Can potentially be used with other applications such as Flow-FISH where fluorescence quantification is required

PDS 821.pdf & PDS 818

 

 

 

 

Quantification System 2: QSC

  • Known quantity of surface attached IgG’s that bind to the Fc region of your conjugated Ab
  • Need to titrate Ab to determine saturation
  • Versatile by allowing investigators the freedom to use fluorochromes of their choosing conjugated to the Ab.

PDS 814.pdf & PDS 818