SiO2 with natural hydroxyl or silanol (SiOH) surface groups for adsorption of nucleotides with low protein binding (see TechNote 104). Non-functionalized silica microspheres are available as ~10% solids (w/w) aqueous suspensions or in the dry form in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.

Catalog Code Diameter (µm) Product Data Sheet Safety Data Sheet
SS02N ≤ 0.49 PDS 702.pdf SDS SG140.pdf or SDS SG141.pdf (dry silica)
SS03N 0.50-0.99 PDS 702.pdf SDS SG140.pdf or SDS SG141.pdf (dry silica)
SS04N 1.00-2.49 PDS 702.pdf SDS SG140.pdf or SDS SG141.pdf (dry silica)
SS05N 2.50-4.99 PDS 702.pdf SDS SG140.pdf or SDS SG141.pdf (dry silica)
SS06N ≥ 5.00 PDS 702.pdf SDS SG140.pdf or SDS SG141.pdf (dry silica)

References

Ferguson JA, Steemers FJ, Walt DR. (2000) High-density fiber-optic DNA random microsphere array. Anal Chem; 72(22):5618-5624.

Lauer S, Goldstein B, Nolan RL, Nolan JP. (2002) Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry; 41(6):1742-51.

Steinberg G, Stromsborg K, Thomas L, Barker D, Zhao C. (2004) Strategies for covalent attachment of DNA to beads. Biopolymers; 73(5):597-605.

B Sharma, P Bugga, L Madison, A Henry, M Blaber, N Greeneltch, ... R. Van Duyne (2016) Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman Spectroscopy  Am Chem Soc, 138 (42), pp 13952–13959

G Steinberg-Tatman,M Huynh, D Barker, C Zhao (2006) Synthetic Modification of Silica Beads That Allows for Sequential Attachment of Two Different Oligonucleotides Bioconjugate Chem 17(3), 841-848

N Clack, K Salaita, J Groves (2008) Electrostatic readout of DNA microarrays with charged microspheres. Nat Biotech 26,825-830

Lu Q, Liu H, Cao T (2013) Efficient Isolation of Bone Marrow Adipocyte Progenitors by Silica Microbeads Incubation. Stem Cells Dev. V22:18

Khan Z, Poetter K, Park DJ.(2008) Enhanced solid phase PCR: mechanisms to increase priming by solid support primers. Anal Biochem. Apr 15;375(2):391-3.

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