Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications we stock an extensive inventory of fluorescent microspheres and offer custom dyeing services.

Europium chelate

Our europium products offer extremely bright fluorescence and exceptional stability, in addition to well-functionalized carboxylated surfaces for the covalent attachment of ligand. Europium Chelate spheres have been utilized for highly sensitive assays (microplate, lateral flow) based on time-resolved fluorescence and as particulate labels in chemiluminence assays. The beads are supplied as ~1% solids (w/v) aqueous suspensions. View excitation & emission spectra.

Catalog Number Fluorescence Color Nominal Diameter Specification Range
FCEU001 Europium Chelate 0.10µm 0.090 - 0.110µm
FCEU002 Europium Chelate 0.20µm 0.190 - 0.210µm
FCEU003 Europium Chelate 0.30µm 0.270 - 0.330µm

references

Liang R-L, Xu X-P, Liu T-C, Zhou J-W, Wang X-G, Ren Z-Q, Hao F, Wu Y-S. (2015) Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips. Anal Chim Acta; 891:277-283.

Soukka T, Paukkunen J, Härmä H, Lönnberg S, Lindroos H, Lövgren. (2001) Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem; 47(4):(1269-1278

Xia X, Xu Y, Ke R, Zhang H, Zou M, Yang W, Li Q. (2013) A highly sensitive nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue. Anal Bioanal Chem; 405(23):7541-4.

Yeo SJ, Bao DT, Seo GE, Bui CT, Kim DTH, Anh NTV, … Park H. (2017). Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles. Scientific Reports, 7, 7933.

Fluorescent Polymer

Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Unless noted otherwise, fluorescent polymer microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Fluorescence Color Nominal Diameter Specification Range
FSDG001 Dragon Green 0.05µm 0.040 - 0.060µm
FSFR001 Flash Red 0.05µm 0.040 - 0.060µm
FSDG002 Dragon Green 0.20µm 0.190 - 0.210µm
FSSY002 Suncoast Yellow 0.20µm 0.190 - 0.210µm
FSFR002 Flash Red 0.20µm 0.190 - 0.210µm
FSDG003 Dragon Green 0.50µm 0.470 - 0.530µm
FSPP003 Plum Purple 0.50µm 0.470 - 0.530µm
FSFR003 Flash Red 0.50µm 0.470 - 0.530µm
FSDG004 Dragon Green 1.00µm 0.95 - 1.05µm
FSEG004 Envy Green 1.00µm 0.95 - 1.05µm
FSPP004 Plum Purple 1.00µm 0.95 - 1.05µm
FSFR004 Flash Red 1.00µm 0.95 - 1.05µm
FSDG005 Dragon Green 2.00µm 1.80 - 2.20µm
FSPP005 Plum Purple 2.00µm 1.80 - 2.20µm
FSFR005 Flash Red 2.00µm 1.80 - 2.20µm
FSDG006 Dragon Green 4.00µm 3.80 - 4.20µm
FSEG006 Envy Green 4.00µm 3.80 - 4.20µm
FSFR006 Flash Red 4.00µm 3.80 - 4.20µm
FSDG007 Dragon Green 7.5µm 7.30 - 7.70µm
FSSY007 Suncoast Yellow 7.5µm 7.30 - 7.70µm
FSFR007 Flash Red 7.5µm 7.30 - 7.70µm
FSEG008 Envy Green 10.0µm 9.50 - 10.50µm
FSDG009 Dragon Green 15.00µm 14.5 - 15.50µm
FSDG011 Dragon Green >25.00µm >25.00µm

References

Lee, M, Reece, P, Marchington, R, Metzger, N, Dholakia, K (2007) Construction and calibration of an optical trap on a fluorescence optical microscope. Nat. Protocols V.2 p3226-3238

Mattheyses, A, Simon, S, Rappoport, J (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist J Cell Sci 123: 3621-3628

Sun, Z, Martinez-Lemus, L, Trache, A, Trzeciakowski, J, Davis, G, Pohl, U, Meininger,G (2005) Mechanical properties of the interaction between fibronectin and α5β1-integrin on vascular smooth muscle cells studied using atomic force microscopy. Am J Physiol Heart Circ Physiol, 289(6) H2526-H2535

Ogilvie, J, Beaurepaire, E, Alexandrou, A, Joffre, M, (2006) Fourier-transform coherent anti-Stokes Raman scattering microscopy. Optics Letters, Optical Society of America, 31(4), p480-2.

Fluorescent Carboxyl Polymer

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Fluorescence Color Nominal Diameter Specification Range
FCDG001 Dragon Green 0.05µm 0.040 - 0.060µm
FCFR001 Flash Red 0.05µm 0.040 - 0.060µm
FCDG002 Dragon Green 0.10µm 0.090 - 0.110µm
FCDG003 Dragon Green 0.20µm 0.190 - 0.210µm
FCSG003

Surf Green

0.20µm 0.190 - 0.210µm
FCFR003 Flash Red 0.20µm 0.190 - 0.210µm
FCGB003 Glacial Blue 0.20µm 0.190 - 0.210µm
FCDG004 Dragon Green 0.40µm 0.370 -0.430µm
FCFR004 Flash Red 0.40µm 0.370 -0.430µm
FCDG005 Dragon Green 0.50µm 0.470 - 0.530µm
FCFR005 Flash Red 0.50µm 0.470 - 0.530µm
FCDG006 Dragon Green 1.0µm 0.95 - 1.05µm
FCEG006 Envy Green 1.0µm 0.95 - 1.05µm
FCSY006 Suncoast Yellow 1.0µm 0.95 - 1.05µm
FCGB006 Glacial Blue 1.0µm 0.95 - 1.05µm
FCFR006 Flash Red 1.0µm 0.95 - 1.05µm
FCSY007 Suncoast Yellow 2.0µm 1.80 - 2.20µm
FCDG008 Dragon Green 5.0µm 4.80 - 5.20µm
FCEG008 Envy Green 5.0µm 4.80 - 5.20µm
FCGB008 Glacial Blue 5.0µm 4.80 - 5.20µm
FCFR008 Flash Red    5.0µm 4.80 - 5.20µm
FCDG009 Dragon Green 10.0µm 9.50 - 10.50µm

Fluorescent Magnetic Polymer

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the polymer matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Description Fluorescence Color Nominal Diameter Specification Range Product Data Sheet Safety Data Sheet
MCDG001 Classical Magnetic Polymer Dragon Green 0.9µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MCFR001 Classical Magnetic Polymer Flash Red 0.9µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEDG001 Encapsulated Magnetic Polymer Dragon Green .86µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEFR001 Encapsulated Magnetic Polymer Flash Red .86µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEDG002 Encapsulated Magnetic Polymer Dragon Green 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MESY002 Encapsulated Magnetic Polymer Suncoast Yellow 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEGB002 Encapsulated Magnetic Polymer Glacial Blue 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
MEFR002 Encapsulated Magnetic Polymer Flash Red 1.63µm 0.50 - 2.00µm PDS 731.pdf SDS MF220.pdf
UMGB001 COMPEL™ Magnetic COOH modified Glacial Blue 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMDG001 COMPEL™ Magnetic COOH modified Dragon Green 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMEG001 COMPEL™ Magnetic COOH modified Envy Green 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMFR001 COMPEL™ Magnetic COOH modified Flash Red 3µm 2.50 - 3.50µm PDS 705.pdf SDS MF221.pdf
UMGB002 COMPEL™ Magnetic COOH modified Glacial Blue 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMDG002 COMPEL™ Magnetic COOH modified Dragon Green 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMFR002 COMPEL™ Magnetic COOH modified Flash Red 6µm 5.50 - 6.50µm PDS 705.pdf SDS MF221.pdf
UMGB003 COMPEL™ Magnetic COOH modified Glacial Blue 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf
UMDG003 COMPEL™ Magnetic COOH modified Dragon Green 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf
UMFR003 COMPEL™ Magnetic COOH modified Flash Red 8µm 7.50 - 8.50µm PDS 705.pdf SDS MF221.pdf

References

Ebert, SN, Taylor, DG, Nguyen, HL, Kodack, DP, Beyers, RJ, Xu, Y, Yang, Z, French, BA (2007) Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques. STEM CELLS 25:2936–2944.

Trost, A, Motloch, K, Bruckner, D, Schroedl, F, Bognera, B, Kaser-Eichberger, A, Runge, … Reitsamer, H.A.(2015) Time-dependent retinal ganglion cell loss, microglial activation and blood-retina-barrier tightness in an acute model of ocular hypertension. Exp Eye Res V136, p59–71

Fluorescent Protein Coated Polymer

Our inventory includes a variety of streptavidin coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions. Please select the catalog code link below or contact our Customer Service Department for specific availability.

Catalog Number Coating / Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
CFDG001 SA / Dragon Green 0.20µm 0.190 - 0.210µm PDS 721.pdf
CFDG002 SA / Dragon Green 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFFR002 SA / Flash Red 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFDG003 SA / Dragon Green 0.50µm 0.470 - 0.530µm PDS 721.pdf
CFDG004 SA / Dragon Green 1.00µm 0.95 - 1.05µm PDS 721.pdf
CFFR004 SA / Flash Red 1.00µm 0.95 - 1.05µm PDS 721.pdf