Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications we stock an extensive inventory of fluorescent microspheres and offer custom dyeing services.

Europium chelate

Our europium products offer extremely bright fluorescence and exceptional stability, in addition to well-functionalized carboxylated surfaces for the covalent attachment of ligand. Europium Chelate spheres have been utilized for highly sensitive assays (microplate, lateral flow) based on time-resolved fluorescence and as particulate labels in chemiluminence assays. The beads are supplied as ~1% solids (w/v) aqueous suspensions. View excitation & emission spectra.

Catalog Code Diameter (µm) Product Data Sheet
FC02F ≤ 0.49 PDS 741.pdf

Fluorescent Polymer

Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Unless noted otherwise, fluorescent polymer microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Diameter (µm) Product Data Sheet
FS02F ≤ 0.49 PDS 731. pdf
FS03F 0.50 - 0.99 PDS 731. pdf
FS04F 1.00 - 1.99 PDS 731. pdf
FS05F 2.00 - 4.99 PDS 731. pdf
FS06F 5.00 - 9.99 PDS 731. pdf
FS07F 10.00 - 24.99 PDS 731. pdf
FS08F ≥ 25.00 PDS 731. pdf

Fluorescent Carboxyl Polymer

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Diameter (µm) Product Data Sheet
FC02F ≤ 0.49 PDS 731.pdf
FC03F 0.50 - 0.99 PDS 731.pdf
FC04F 1.00 - 1.99 PDS 731.pdf
FC05F 2.00 - 4.99 PDS 731.pdf
FC06F 5.00 - 9.99 PDS 731.pdf

Fluorescent Magnetic Polymer

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the polymer matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Description Diameter (µm) Product Data Sheet Safety Data Sheet
MC03F Classical Magnetic Polymer, fluorescent 0.50 - 0.99 PDS 731.pdf SDS MF220.pdf
ME03F Encapsulated Magnetic Polymer, fluorescent 0.50 - 0.99 PDS 731.pdf SDS MF220.pdf
ME04F Encapsulated Magnetic Polymer, fluorescent 1.00 - 1.99 PDS 731.pdf SDS MF220.pdf
UMC3F COMPEL™ Magnetic COOH modified, fluorescent 3.00, 6.00 PDS 705.pdf SDS MF221.pdf
UMC4F COMPEL™ Magnetic COOH modified, fluorescent 8.00 PDS 705.pdf SDS MF221.pdf

References

Ebert, SN, Taylor, DG, Nguyen, HL, Kodack, DP, Beyers, RJ, Xu, Y, Yang, Z, French, BA (2007) Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques. STEM CELLS 25:2936–2944.

Trost, A, Motloch, K, Bruckner, D, Schroedl, F, Bognera, B, Kaser-Eichberger, A, Runge, … Reitsamer, H.A.(2015) Time-dependent retinal ganglion cell loss, microglial activation and blood-retina-barrier tightness in an acute model of ocular hypertension. Exp Eye Res V136, p59–71

Fluorescent Protein Coated Polymer

Our inventory includes a variety of streptavidin coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions. Please select the catalog code link below or contact our Customer Service Department for specific availability.

Catalog Code Description Product Data Sheet
CP01F Fluorescent Protein Coated Polymer PDS 721.pdf