Oh, how we love to accessorize!

Bangs Laboratories offers a variety of accessory products for use in microparticle reagent development. The collection includes crosslinking reagents and surfactants, as well as solutions and buffers for microsphere coating and storage. These products complement our extensive catalog of polymer, silica, and magnetic microparticles, and also offer a convenient means to replenish individual components of coupling kits.

Buffers and Solutions

Bangs Bead Coupling Buffers are ready-to-use buffers that are available in a variety of pH levels (4.5 to 9.0). They can be used as coupling or wash buffers for plain, dyed, or functionalized polymer microspheres. Our Bangs Bead Storage Buffer is available in two pH levels: 7.4 and 8.5.

Our Bead Solution is a ready-to-use aqueous suspending solution for the dilution and/or storage of your uncoated plain, dyed, or functionalized polymer microspheres (both carboxylated or aminated). An antimicrobial agent deters microbial contamination, and stabilizers promote suspension dispersity, peace of mind, and harmonious accord in the laboratory.

Catalog Code Description pH Volume (mL) Product Data Sheet Material Safety Data Sheet
BUFF1 Bangs Bead Coupling Buffer 4.5 250, 500, 1000, 2000 PDS 738.pdf SDS AR405.pdf
BUFF2 Bangs Bead Coupling Buffer 6.0 250, 500, 1000, 2000 PDS 738.pdf SDS AR406.pdf
BUFF3 Bangs Bead Coupling Buffer 7.4 250, 500, 1000, 2000 PDS 738.pdf SDS AR407.pdf
BUFF4 Bangs Bead Coupling Buffer 9.0 250, 500, 1000, 2000 PDS 738.pdf SDS AR408.pdf
BUFF5 Bangs Bead Storage Buffer 7.4 250, 500, 1000, 2000 PDS 738.pdf SDS AR409.pdf
BUFF6 Bangs Bead Storage Buffer 8.5 250, 500, 1000, 2000 PDS 738.pdf SDS AR410.pdf
SOLN1 Bangs Bead Solution - 500, 1000, 2000 PDS 738.pdf SDS AR411.pdf

Coupling Reagents

Microspheres are routinely coated with ligands such as antibodies, oligonucleotides, and peptides, for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and glutaraldehyde are two of the most common crosslinkers used in bead coating protocols.

EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres, while Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.

Catalog Code Description Volume Product Data Sheet Material Safety Data Sheet
AA010 DEPC - Carbodiimide (EDAC) 1g & 5g PDS 740.pdf SDS AR400.pdf
AA012 Glutaraldehyde, EM Grade, 25% 10 × 10 mL (ampoules) PDS 740.pdf SDS AR401.pdf

Surfactants

Surfactants are commonly utilized with microspheres composed of hydrophobic polymer matrices such as polystyrene to facilitate bead wetting and to deter or treat aggregation. Common concentrations are ~0.1% for uncoated polymer beads and ~0.01% in the storage buffer of coated polymer, magnetic, and silica beads.

Triton® X-100 and Tween® 20 are nonionic surfactants used in the storage buffers of coated bead suspensions, while Sodium Dodecyl Sulfate (SDS) is an anionic surfactant that decreases hydrophobicity and participates in charge stabilization of the suspension.

Catalog Code Description Volume (g) Product Data Sheet Material Safety Data Sheet
AA014 Triton X-100 Nonionic Surfactant 10 PDS 739.pdf SDS AR402.pdf
AA016 Tween® 20 Nonionic Surfactant 10 PDS 739.pdf SDS AR403.pdf
AA018 Sodiom Dodecyl Sulfate Anionic Surfactant 10 PDS 739.pdf SDS AR404.pdf