I’m looking to do fluorescence quantitation using your Quantum MESF kits. One concern I had however was that my unlabeled cells have a lower fluorescence intensity than the blank bead. Why is that, and what do I do in this instance?

Our Quantum MESF and Quantum Simply Cellular beads are composed of polystyrene that have some level of autofluorescence varying based on the laser/channel in use. If you have control cells with lower MFI (mean fluorescence intensity) values than our...

See how our MESF & QSC kits support quantitative flow cytometry in cancer research & immunotherapy development.

Enjoy this curated listing of references related to cancer research & immunotherapy development.Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist....

My antibody isn’t binding to the Quantum Simply Cellular kit I just received. Can you help?

A first step is to confirm the host species of the primary antibody, as the most common reason for a complete lack of binding is antibody mismatch. We sometimes see clients mistakenly use the target species (of cells) when selecting the QSC kit,...

What are you using to measure results in your flow cytometric study?

See how the Quantum MESF system has been used to standardize studies and quantify results in a range of applications:Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, et al. (2013) Manufacture of Clinical-Grade CD19-Specific T Cells Stably...

I’m using your Quantum Simply Cellular (QSC) beads to quantitate cell surface receptors, but I’m fixing my cells as part of my standard prep. Do I also need to fix the beads as well?

Sample handling/prep can certainly play a large role in quantitative flow cytometry, although we recommend against using fixation steps for our beads, as many of the fixatives are known solvents of our polystyrene particles (e.g. formaldehyde or...

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Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links.  If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!
 
 
 
 
 
 
 

For more information, see our flow tutorial page or our entire library in Tech Support