I ran some beads and noticed there are two or three populations. I thought the beads were only a single size? What’s going on?

What this user is noticing is likely aggregation of the beads. FSC/SSC (forward scatter / side scatter) dot plots provide general information in terms of size (FSC) and granularity (SSC). When two particles are stuck together, the instrument will...

Do you have tools for assessing receptor occupancy (RO) in biopharmaceutical development?

Flow cytometry is an important technology for conducting receptor occupancy assays in drug / biotherapeutic development, including free receptor, total receptor, bound receptor and receptor modulation formats. Our Quantum MESF and Quantum Simply...

Small Bead Kits for microvesicle and exosome analysis.

Interest in microvesicles and exosomes has rapidly risen over the past decade as a means to study cellular interactions, immune behavior, and various disease states. Flow cytometry, while traditionally focused on cell-based analysis, is a powerful...

I have to use an indirect labeling method for my cells, is this compatible with Quantum Simply Cellular beads?

A very common question, and one that we speak at length with various users about.Whenever possible, we recommend that a direct labeling strategy is used with cell samples and Quantum Simply Cellular (QSC) beads. This is advantageous as indirect...

Quantum MESF for Intracellular Fluorescence: Telomere Content Measurement

From time to time, we’re asked about whether we have tools to help with quantitation of intracellular markers, probes, etc.  To which we respond: "Heck yeah…!”As cytometers don’t mind whether cellular fluorescence is internal or external, our...

Pages

Flow Cytometry Resources

We’re proud to continue the tradition of standardization in flow cytometry that our predecessor Flow Cytometry Standards Corporation began, and to contribute our expertise in the development and manufacture of specialty microsphere products. We invite you to check out our new blog and to browse our library of technical documents, tutorials, and useful links.  If any questions remain unanswered, please feel free to submit a question to Ask The Doc or give us a call—we are happy to help!
 
 
 
 
 
 
 

For more information, see our flow tutorial page or our entire library in Tech Support