Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications we stock an extensive inventory of fluorescent microspheres and offer custom dyeing services. See spectra for available fluorescent dyes.

Europium chelate

Our europium products offer extremely bright fluorescence and exceptional stability, in addition to well-functionalized carboxylated surfaces for the covalent attachment of ligand. Europium Chelate spheres have been utilized for highly sensitive assays (microplate, lateral flow) based on time-resolved fluorescence and as particulate labels in chemiluminence assays. The beads are supplied as ~1% solids (w/v) aqueous suspensions. View excitation & emission spectra.

Catalog Code Diameter (µm) Product Data Sheet
FC02F ≤ 0.49 PDS 741.pdf

references

Liang R-L, Xu X-P, Liu T-C, Zhou J-W, Wang X-G, Ren Z-Q, Hao F, Wu Y-S. (2015) Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips. Anal Chim Acta; 891:277-283.

Soukka T, Paukkunen J, Härmä H, Lönnberg S, Lindroos H, Lövgren. (2001) Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem; 47(4):(1269-1278

Xia X, Xu Y, Ke R, Zhang H, Zou M, Yang W, Li Q. (2013) A highly sensitive nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue. Anal Bioanal Chem; 405(23):7541-4.

Fluorescent Polymer

Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Unless noted otherwise, fluorescent polymer microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Diameter (µm) Product Data Sheet
FS02F ≤ 0.49 PDS 731. pdf
FS03F 0.50 - 0.99 PDS 731. pdf
FS04F 1.00 - 1.99 PDS 731. pdf
FS05F 2.00 - 4.99 PDS 731. pdf
FS06F 5.00 - 9.99 PDS 731. pdf
FS07F 10.00 - 24.99 PDS 731. pdf
FS08F ≥ 25.00 PDS 731. pdf

References

Lee, M, Reece, P, Marchington, R, Metzger, N, Dholakia, K (2007) Construction and calibration of an optical trap on a fluorescence optical microscope. Nat. Protocols V.2 p3226-3238

Mattheyses, A, Simon, S, Rappoport, J (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist J Cell Sci 123: 3621-3628

Sun, Z, Martinez-Lemus, L, Trache, A, Trzeciakowski, J, Davis, G, Pohl, U, Meininger,G (2005) Mechanical properties of the interaction between fibronectin and α5β1-integrin on vascular smooth muscle cells studied using atomic force microscopy. Am J Physiol Heart Circ Physiol, 289(6) H2526-H2535

Ogilvie, J, Beaurepaire, E, Alexandrou, A, Joffre, M, (2006) Fourier-transform coherent anti-Stokes Raman scattering microscopy. Optics Letters, Optical Society of America, 31(4), p480-2.

Fluorescent Carboxyl Polymer

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes. See also our Quantumplex™ kits for suspension array development.

Catalog Code Diameter (µm) Product Data Sheet
FC02F ≤ 0.49 PDS 731.pdf
FC03F 0.50 - 0.99 PDS 731.pdf
FC04F 1.00 - 1.99 PDS 731.pdf
FC05F 2.00 - 4.99 PDS 731.pdf
FC06F 5.00 - 9.99 PDS 731.pdf

Fluorescent Magnetic Polymer

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the polymer matrix. For specific information on the use of fluorescent magnetic particles for cell labeling & tracking, see TSD 0748. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes.

Catalog Code Description Diameter (µm) Product Data Sheet Safety Data Sheet
MC03F Classical Magnetic Polymer, fluorescent 0.50 - 0.99 PDS 731.pdf SDS MF220.pdf
ME03F Encapsulated Magnetic Polymer, fluorescent 0.50 - 0.99 PDS 731.pdf SDS MF220.pdf
ME04F Encapsulated Magnetic Polymer, fluorescent 1.00 - 1.99 PDS 731.pdf SDS MF220.pdf
UMC3F COMPEL™ Magnetic COOH modified, fluorescent 3.00, 6.00 PDS 705.pdf SDS MF221.pdf
UMC4F COMPEL™ Magnetic COOH modified, fluorescent 8.00 PDS 705.pdf SDS MF221.pdf

References

Hinds, K, Hill, J, Shapiro, E, Laukkanen, M, Alfonso C. Silva, Combs,…Dunbar, C (2003) Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. Blood Aug 102 (3) 867-872

Shapiro, E M, Skrtic, S, Koretsky, AP (2005) Sizing it up: Cellular MRI using micron-sized iron oxide particles. Magn. Reson. Med., 53: 329–338

Rohani, R, de Chickera, SN, Willert, C, Chen, Y, Dekaban, G, Foster, P (2011) In Vivo Cellular MRI of Dendritic Cell Migration Using Micrometer-Sized Iron Oxide (MPIO) Particles Mol Imaging Biol 13: 679

Slotkin,J, Cahill, K, Tharin, S, Shapiro, EM (2207) Cellular Magnetic Resonance Imaging: Nanometer and Micrometer Size Particles for Noninvasive Cell Localization Neurotherapeutics, V4,3,P428-433

Fluorescent Protein Coated Polymer

Our inventory includes a variety of streptavidin coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions. Please select the catalog code link below or contact our Customer Service Department for specific availability. See spectra for available fluorescent dyes.

Catalog Code Description Product Data Sheet
CP01F Fluorescent Protein Coated Polymer PDS 721.pdf

StarLight™ Calibration Slides

Our StarLight™ Calibration Slides feature vibrant ~6µm fluorescent microspheres dyed with a single fluorophore for basic imaging checks and calibration. Our four standard versions are appropriate for use with common microscope filter sets: Glacial Blue (360, 450), Dragon Green (480, 520), Envy Green (525, 565), and Flash Red (660, 690). The calibration slides are available individually or as the full 4-slide collection. See fluorophore spectra here.
Catalog Code Description Product Data Sheet
SL1GB StarLight™ Calibration Slide - Glacial Blue PDS 857.pdf
SL1DG StarLight™ Calibration Slide - Dragon Green PDS 857.pdf
SL1EG StarLight™ Calibration Slide - Envy Green PDS 857.pdf
SL1FR StarLight™ Calibration Slide - Flash Red PDS 857.pdf
SL04K StarLight™ Collection - Slide 4 -Pack PDS 857.pdf

Dragon Green Intensity Standard

The Dragon Green Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets. View excitation and emission spectra.

Catalog Code Description Product Data Sheet Safety Data Sheet
DG06M Dragon Green Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Flash Red Intensity Standard

The Flash Red Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard. View excitation and emission spectra.

Catalog Code Description Product Data Sheet Safety Data Sheet
FR06M Flash Red Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Flow Cytometry

See also our extensive Flow Cytometry products.