We manufacture a number of standards that are intended for use with either image-based cell viability analyzers (e.g. trypan blue, Vi-CELL®) or flow cytometers (DAPI, propidium iodide, LIVE/DEAD® stains, etc) that are important to bioprocessing and research programs.

ViaCheck™ Viability and Concentration Standards

Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Viability Controls are supplied at ~1.0 x 106 beads / mL and are available in a range of live/dead ratios. ViaCheck™ Concentration Controls are supplied at the noted concentrations.

Catalog Code Description Volume (mL) Product Data Sheet Safety Data Sheet
VC10B ViaCheck™ 0% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC25B ViaCheck™ 25% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC20B ViaCheck™ 50% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC30B ViaCheck™ 75% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC40B ViaCheck™ 90% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC50B ViaCheck™ 100% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC60N ViaCheck™ Concentration Control (1 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC70N ViaCheck™ % Concentration Control (4 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC80N ViaCheck™ % Concentration Control (8 × 106) 20 PDS 711.pdf SDS ST353.pdf

References

Kaminski RW, Wu M, Turbyfill  KR, Clarkson K, Tai B, Bourgeois AL, Van De Verg LL, Walker RI, Oaks EV. (2014) Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine. Clin Vaccine Immunol; 21(3):366-382.

Cadena-Herrera D, Esparza-De Lara JE, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E. (2015) Validation of three viable-cell counting methods: manual, semi-automated, and automated. Biotechnol Reports; 7:9-16.

Lew C, Gomez J, Rhyner M (2012) Instrument-to-instrument Variability in the Vi-CELL Automated Viability Analyzer. Beckman Coulter Life Sciences

Viability Dye Compensation Standard - Flow

Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain.

Catalog Code Description Product Data Sheet Safety Data Sheet
450 Viability Dye Compensation Standard, 4µm PDS 853.pdf SDS ST340.pdf
451 Viability Dye Compensation Standard, 8µm PDS 853.pdf SDS ST340.pdf

See also our range of Fluorescence Reference Standards for beads labeled with common viability dyes such as DAPI, Propidium Iodide, etc.