We manufacture a number of standards that are intended for use with either image-based cell viability analyzers (e.g. trypan blue, Vi-CELL®) or flow cytometers (DAPI, propidium iodide, LIVE/DEAD® stains, etc) that are important to bioprocessing and research programs.

ViaCheck™ Viability and Concentration Standards

Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Viability Controls are supplied at ~1.0 x 106 beads / mL and are available in a range of live/dead ratios. ViaCheck™ Concentration Controls are supplied at the noted concentrations.

Catalog Code Description Volume (mL) Product Data Sheet Safety Data Sheet
VC10B ViaCheck™ 0% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC25B ViaCheck™ 25% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC20B ViaCheck™ 50% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC30B ViaCheck™ 75% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC40B ViaCheck™ 90% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC50B ViaCheck™ 100% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC60N ViaCheck™ Concentration Control (1 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC70N ViaCheck™ % Concentration Control (4 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC80N ViaCheck™ % Concentration Control (8 × 106) 20 PDS 711.pdf SDS ST353.pdf

References

Kaminski RW, Wu M, Turbyfill  KR, Clarkson K, Tai B, Bourgeois AL, Van De Verg LL, Walker RI, Oaks EV. (2014) Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine. Clin Vaccine Immunol; 21(3):366-382.

Cadena-Herrera D, Esparza-De Lara JE, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E. (2015) Validation of three viable-cell counting methods: manual, semi-automated, and automated. Biotechnol Reports; 7:9-16.

Lew C, Gomez J, Rhyner M (2012) Instrument-to-instrument Variability in the Vi-CELL Automated Viability Analyzer. Beckman Coulter Life Sciences

Trypan Blue

Trypan blue exclusion is a common method for the determination of cell viability. It is used extensively in biopharmaceutical cell culture programs, and for a range of research studies including apoptosis, cytopathic effects of viral infection, and effects of sample processing methods on cell viability and concentration. Our solution is suitable for traditional manual staining and hemocytometer counting. NOTE: This product is not intended to be a plug-in replacement bottle for automated cell viability analyzers. Instrument manufacturers please contact us for bulk/OEM packaging if this is your interest.

 special oFfer!

We are so excited about our new product Trypan Blue, for a limited time we are offering a free bottle with any ViaCheck Viability or ViaCheck Concentration order. Place an order for any ViaCheck product. Use code word: BLUE in the comments section of your online order form or when ordering by phone, and we’ll send you a 125mL bottle of Trypan blue FREE!

Catalog Code Description Volume (mL) Product Data Sheet Safety Data Sheet
AA020 Trypan Blue 0.4% Solution 125mL PDS 748.pdf SDS AR414.pdf
AA021 Trypan Blue 0.4% Solution 250mL PDS 748.pdf SDS AR414.pdf

Viability Dye Compensation Standard - Flow

Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or similarly-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain.

Catalog Code Description Product Data Sheet Material Safety Data Sheet
450 Viability Dye Compensation Standard, 4µm PDS 853.pdf SDS ST340.pdf
451 Viability Dye Compensation Standard, 8µm PDS 853.pdf SDS ST340.pdf

See also our range of Fluorescence Reference Standards for beads labeled with common viability dyes such as DAPI, Propidium Iodide, etc.