We manufacture a number of standards that are intended for use with either image-based cell viability analyzers (e.g. trypan blue, Vi-CELL®) or flow cytometers (DAPI, propidium iodide, LIVE/DEAD® stains, etc) that are important to bioprocessing and research programs.
ViaCheck™ Viability and Concentration Standards
Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Viability Controls are supplied at ~1.0 x 106 beads / mL and are available in a range of live/dead ratios. ViaCheck™ Concentration Controls are supplied at the noted concentrations.
|Catalog Code||Description||Volume (mL)||Product Data Sheet||Safety Data Sheet|
|VC10B||ViaCheck™ 0% Viability Control||20||PDS 706.pdf||SDS ST352.pdf|
|VC25B||ViaCheck™ 25% Viability Control||20||PDS 706.pdf||SDS ST352.pdf|
|VC20B||ViaCheck™ 50% Viability Control||20||PDS 706.pdf||SDS ST352.pdf|
|VC30B||ViaCheck™ 75% Viability Control||20||PDS 706.pdf||SDS ST352.pdf|
|VC40B||ViaCheck™ 90% Viability Control||20||PDS 706.pdf||SDS ST352.pdf|
|VC50B||ViaCheck™ 100% Viability Control||20||PDS 706.pdf||SDS ST352.pdf|
|VC60N||ViaCheck™ Concentration Control (1 × 106)||20||PDS 711.pdf||SDS ST353.pdf|
|VC70N||ViaCheck™ % Concentration Control (4 × 106)||20||PDS 711.pdf||SDS ST353.pdf|
|VC80N||ViaCheck™ % Concentration Control (8 × 106)||20||PDS 711.pdf||SDS ST353.pdf|
Kaminski RW, Wu M, Turbyfill KR, Clarkson K, Tai B, Bourgeois AL, Van De Verg LL, Walker RI, Oaks EV. (2014) Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine. Clin Vaccine Immunol; 21(3):366-382.
Cadena-Herrera D, Esparza-De Lara JE, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E. (2015) Validation of three viable-cell counting methods: manual, semi-automated, and automated. Biotechnol Reports; 7:9-16.
Lew C, Gomez J, Rhyner M (2012) Instrument-to-instrument Variability in the Vi-CELL Automated Viability Analyzer. Beckman Coulter Life Sciences
Viability Dye Compensation Standard - Flow
Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain.
|Catalog Code||Description||Product Data Sheet||Safety Data Sheet|
|450||Viability Dye Compensation Standard, 4µm||PDS 853.pdf||SDS ST340.pdf|
|451||Viability Dye Compensation Standard, 8µm||PDS 853.pdf||SDS ST340.pdf|
See also our range of Fluorescence Reference Standards for beads labeled with common viability dyes such as DAPI, Propidium Iodide, etc.