Affinity Ligand Coated Polystyrene

Affinity Ligand Coated Microspheres

When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of Ab/Ag complexes in immunoprecipitation.

Streptavidin

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications.. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

TechNote 101, ProActive® Microspheres
PDS 721, Streptavidin Coated Microspheres

Streptavidin Polystyrene

Streptavidin Magnetic Polymer

The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL.

See spectra for available fluorescent dyes.
PDS 721, Streptavidin Coated Microspheres
TechNote 102, Magnetic Microspheres

 Streptavidin Fluorescent Polystyrene

Streptavidin-coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions.

PDS 721, Streptavidin Coated Microspheres
PDS 731, Fluorescent Microspheres

Streptavidin Dyed Polystyrene

Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.

PDS 721, Streptavidin Microspheres
PDS 717, Visibly Dyed Microspheres

Biotin

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions.

See TechNote 101, ProActive^® Microspheres, for sample applications and procedures.
PDS 724, Biotin Coated Microspheres

Protein A and Protein G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

TechNote 101, ProActive Microspheres
PDS 722, Protein A Coated Microspheres
TSD0025, Strengths of Protein A and G Antibody Affinities