Affinity Ligand Coated Microspheres

Affinity binding ligands can aid in the rapid development or streamlined production of microsphere reagents. They also provide the opportunity for optimal orientation of the ligand, as with end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Affinity ligands are also used extensively in isolation and purifications. Streptavidin-coated microspheres are often used to capture biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for immunoprecipitation and the purification of antibodies and Fc-tagged fusion proteins. Lectin-coated spheres (e.g. WGA, ConA) are used to bind glycoproteins, glycolipids and other glycan-presenting targets.

Streptavidin

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, Affinity Ligand coated microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® magnetic particle offerings.

Catalog Code Description Product Data Sheet
CM01N Magnetic / Streptavidin PDS 721.pdf
CP01N Polymer / Streptavidin PDS 721.pdf
CP01F Fluorescent Polymer / Streptavidin PDS 721.pdf
CP01* Dyed Polymer / Streptavidin PDS 721.pdf
CS01N Steptavidin-Coated Silica PDS 702.pdf
PMS1N ProMag™ 1 Series • Streptavidin PDS 715.pdf
PMS3HP ProMag™ HP 3 Series • Streptavidin PDS 743.pdf
PMS3N ProMag™ 3 Series • Streptavidin PDS 715.pdf
21950 Streptavidin Magnetic Sample Pack
PMS1N, ProMag 1 Series SA - 1mL (1% solids)
PMS3HP, ProMag HP 3μm SA - 1mL (1% solids)
BP628, BioMagPlus SA - 2mL (5mg / mL)
PDS 749.pdf

* The final character of the catalog code denotes color: B = Blue, R = Red, O = Orange, K = Black, V = Purple, G = Green, F = Fluorescent, N=None.

References

Blumberg, S, Gajraj, A, Pennington, M, Meiners, J (2005) Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy. Biophysical Journal V89(2)p1272–1281

Wang J, Xu D, Kawde A, Polsky R (2001) Metal Nanoparticle-Based Electrochemical Stripping Potentiometric Detection of DNA Hybridization Anal Chem. 73(22), 5576-5581

CP01B, CP01R, CP01O, CP01K, CP01V, CP01G, CP01F, CP01N, CP01, CM01N

Biotin

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See TechNote 101, Affinity Ligand coated microspheres, for sample applications and procedures. See also our BioMag® offerings.

Catalog Code Description Product Data Sheet
CP10N Polymeric / Biotin PDS 724.pdf
CP10

Protein A and Protein G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, Affinity Ligand coated microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® offerings.

Catalog Code Description Product Data Sheet
CM02N Magnetic / Protein A PDS 722.pdf
CP02N Polymer  / Protein A PDS 722.pdf
PMG3N ProMag™ (Magnetic) / Protein G PDS 722.pdf
CM02, CP02

BioMag® Secondary Ab

BioMag® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles.

Catalog Code Description Concentration (mg / mL) Volume (mL) Product Data Sheet Safety Data Sheet
BM563 BioMag®  Goat anti-Human IgG 1 50 PDS 563.pdf SDS MB234.pdf
BM562 BioMag® Goat anti-Human IgG (Fc Specific) 5 50 PDS 562.pdf SDS MB233.pdf
BM561 BioMag® Goat anti-Human IgM 1 50 PDS 561.pdf SDS MB235.pdf
BM549 BioMag® Goat anti-Mouse IgG 1 50 & 500 PDS 549.pdf SDS MB234.pdf
BM550 BioMag® Goat anti-Mouse IgG (Fc Specific) 1 50 & 500 PDS 550.pdf SDS MB233.pdf
BP619 BioMag®Plus Goat anti-Mouse IgG 1 50 PDS 619.pdf SDS MB234.pdf
BP612 BioMag®Plus Goat anti-Mouse IgG Antibody Coupling Kit 1 25 PDS 619.pdf

SDS MB234.pdf
SDS MB255 Wash/Coupling Buffer.pdf

BM558 BioMag® Goat anti-Mouse IgM 1 50 & 500 PDS 558.pdf SDS MB234.pdf
BM559 BioMag® Goat anti-Rabbit IgG 1 50 & 500 PDS 559.pdf SDS MB234.pdf
BM560 BioMag® Goat anti-Rat IgG 1 50 & 500 PDS 560.pdf SDS MB234.pdf
BM548 BioMag® Goat anti-Rat IgG (Fc specific) 5 50 & 500 PDS 548.pdf SDS MB233.pdf
BM557 BioMag® Goat anti-Rat IgM 5 50 & 500 PDS 557.pdf SDS MB233.pdf

BioMag® Lectin

Our ConA- and WGA coated BioMag®Plus microparticles provide a convenient means for isolating glycoproteins from serum or cell lysate, and for investigating other lectin/glycan-mediated processes. The BioMag®Plus magnetic particle format provides high surface area, and permits easy and efficient separations.

Catalog Code Description Concentration (mg/mL) Volume (mL) Product Data Sheet Safety Data Sheet
BP530 BioMag®Plus Wheat Germ Agglutinin 5 3, 10 PDS 716.pdf SDS MB233.pdf
BP531 BioMag® Plus Concanavalin A 5 3, 10 PDS 720.pdf SDS MB237.pdf