Affinity Ligand Coated Microspheres

Affinity binding ligands can aid in the rapid development or streamlined production of microsphere reagents. They also provide the opportunity for optimal orientation of the ligand, as with end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Affinity ligands are also used extensively in isolation and purifications. Streptavidin-coated microspheres are often used to capture biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for immunoprecipitation and the purification of antibodies and Fc-tagged fusion proteins. Lectin-coated spheres (e.g. WGA, ConA) are used to bind glycoproteins, glycolipids and other glycan-presenting targets.

Streptavidin

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, Affinity Ligand coated microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® magnetic particle offerings.

Streptavidin Polymer

Catalog Number Catalog Code Nominal Diameter Specification Range Product Data Sheet
CP01000 CP01N 0.10µm 0.090 - 0.110µm PDS 721.pdf
CP01001 CP01N 0.20µm 0.190 - 0.210µm PDS 721.pdf
CP01003 CP01N 0.50µm 0.470 - 0.530µm PDS 721.pdf
CP01004 CP01N 1.00µm 0.95 - 1.05µm PDS 721.pdf
CP01005 CP01N 3.0µm 2.80 - 3.20µm PDS 721.pdf
CP01006 CP01N 5.00µm 4.80 - 5.20µm PDS 721.pdf
CP01007 CP01N 10.00µm 9.50 - 10.50µm PDS 721.pdf
CP01008 CP01N 15.0µm 14.50 - 15.50µm PDS 721.pdf

References

Nandin IS, Fong C, Deantonio C, Torreno-Pina J, Pecetta S, Maldonado P, … Batista, F.(2017) Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies. J Exp Med.20170633.

Wu SJ,Chan A, Kado CI. (2004) Detection of PCR amplicons from bacterial pathogens using microsphere agglutination J Microbiol Methods Vol 56, Issue 3, 395-400 (5.7µm Streptavidin-coated microspheres)

Walter S, HerrgenL, Schoor O, Jung G, Wernet D, Bühring HJ, … Stevanović S.(2003) Cutting Edge: Predetermined Avidity of Human CD8 T Cells Expanded on Calibrated MHC/Anti-CD28-Coated Microspheres. J Immunol. 171(10)4974-4978 (5.6µm Streptavidin as artificial-antigen presenting cells [aAPC])

Streptavidin Magnetic Polymer

The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes. See also our BioMag® magnetic particle offerings.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available.  
Catalog Number Catalog Code Description Nominal Diameter Specification Range Product Data Sheet
CMC0100 CM01N SA / Classical Magnetic Polymer 0.2 - 0.5µm 0.2 - 0.5µm PDS 721.pdf
CMC0101 CM01N SA / Classical Magnetic Polymer 0.9µm 0.7 - 1.2µm PDS 721.pdf
CME0101 CM01N SA / Encapsulated Magnetic Polymer 0.86µm 0.7 - 1.2µm PDS 721.pdf
UMC0100 CM01N SA/ COMPEL 3µm 2.50 - 3.50µm PDS 721.pdf
UMC0101 CM01N SA / COMPEL 6µm 5.50 - 6.50µm PDS 721.pdf
UMC0102 CM01N SA / COMPEL 8µm 7.50 - 8.50µm PDS 721.pdf
Catalog Number Description Product Data Sheet Safety Data Sheet
PMS1N ProMag™ 1 Series • Streptavidin PDS 715.pdf SDS CG154.pdf
PMS3HP ProMag™ HP 3 Series • Streptavidin PDS 743.pdf SDS CG154.pdf
PMS3N ProMag™ 3 Series • Streptavidin PDS 715.pdf SDS CG154.pdf
21950 Streptavidin Magnetic Sample Pack
PMS1N, ProMag 1 Series SA - 1mL (1% solids)
PMS3HP, ProMag HP 3μm SA - 1mL (1% solids)
BP628, BioMagPlus SA - 2mL (5mg / mL)
PDS 749.pdf

SDS CG154.pdf

SDS MB232.pdf

 Streptavidin Fluorescent Polymer 

Catalog Number Catalog Code Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
CFDG001 CP01F SA / Dragon Green 0.20µm 0.190 - 0.210µm PDS 721.pdf
CFDG002 CP01F SA / Dragon Green 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFFR002 CP01F SA / Flash Red 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFDG003 CP01F SA / Dragon Green 0.50µm 0.470 - 0.530µm PDS 721.pdf
CFDG004 CP01F SA / Dragon Green 1.00µm 0.95 - 1.05µm PDS 721.pdf
CFFR004 CP01F SA / Flash Red 1.00µm 0.95 - 1.05µm

PDS 721.pdf

Streptavidin Dyed Polymer

Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.

Catalog Number Catalog Code Coating / Dye Nominal Diameter Specification Range
CDCR001 CP01* SA / Crimson Red 0.20µm 0.190 - 0.210µm
CDCB001 CP01* SA / Cabo Blue 0.20µm 0.190 - 0.210µm

Streptavidin Silica

SiO2 with a streptavidin-coated surface for the attachment of biomolecules (See also PDS 721.pdf). Silica streptavidin microspheres are sold in these standard amounts: 1mL, 2mL, 5mL, and 10mL. Available at 1% solids (w/v), in three nominal mean diameters: ~0.5µm, ~1.0µm, and ~5.0µm.

Catalog Number Catalog Code Nominal Diameter Specification Range Product Data Sheet Safety Data Sheet
CS01000 CS01N 0.5µm 0.460 - 0.540µm PDS 702.pdf SDS SG142.pdf
CS01001 CS01N 1.0µm 0.95 - 1.05µm PDS 702.pdf SDS SG142.pdf
CS01002 CS01N 5.00µm 4.80 - 5.20µm PDS 702.pdf SDS SG142.pdf

References

Blumberg, S, Gajraj, A, Pennington, M, Meiners, J (2005) Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy. Biophysical Journal V89(2)p1272–1281

Wang J, Xu D, Kawde A, Polsky R (2001) Metal Nanoparticle-Based Electrochemical Stripping Potentiometric Detection of DNA Hybridization Anal Chem. 73(22), 5576-5581

CP01B, CP01R, CP01O, CP01K, CP01V, CP01G, CP01F, CP01N, CP01, CM01N

Biotin

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See TechNote 101, Affinity Ligand coated microspheres, for sample applications and procedures. See also our BioMag® offerings.

Catalog Number Catalog Code Description Nominal Diameter Specification Range Product Data Sheet
CP10000 CP10N Polymeric / Biotin 10µm 9.50 - 10.50µm PDS 724.pdf
CP10

Protein A and Protein G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, Affinity Ligand coated microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® offerings.

Catalog Number Catalog Code Description Nominal Diameter Specification Range Product Data Sheet
CME0200 CM02N Encapsulated Magnetic Polymer / Protein A 0.86µm 0.7 - 1.2µm PDS 722.pdf
CP02000 CP02N Polymer / Protein A 1.00µm 0.95-1.05µm PDS 722.pdf
CP02001 CP02N Polymer / Protein A 5.00µm 4.80-5.20µm PDS 722.pdf
CP02002 CP02N Polymer / Protein A 10.0µm 9.50-10.50µm PDS 722.pdf
PMG3N PMG3N ProMag™ (Magnetic) / Protein G 3µm 2.70 - 3.30µm PDS 722.pdf
CM02, CP02

BioMag® Secondary Ab

BioMag® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles.

Catalog Number Description Concentration (mg / mL) Volume (mL) Product Data Sheet Safety Data Sheet
BM563 BioMag®  Goat anti-Human IgG 1 50 PDS 563.pdf SDS MB234.pdf
BM562 BioMag® Goat anti-Human IgG (Fc Specific) 5 50 PDS 562.pdf SDS MB233.pdf
BM561 BioMag® Goat anti-Human IgM 1 50 PDS 561.pdf SDS MB235.pdf
BM549 BioMag® Goat anti-Mouse IgG 1 50 & 500 PDS 549.pdf SDS MB234.pdf
BM550 BioMag® Goat anti-Mouse IgG (Fc Specific) 1 50 & 500 PDS 550.pdf SDS MB233.pdf
BP619 BioMag®Plus Goat anti-Mouse IgG 1 50 PDS 619.pdf SDS MB234.pdf
BP612 BioMag®Plus Goat anti-Mouse IgG Antibody Coupling Kit 1 25 PDS 619.pdf

SDS MB234.pdf
SDS MB255 Wash/Coupling Buffer.pdf

BM558 BioMag® Goat anti-Mouse IgM 1 50 & 500 PDS 558.pdf SDS MB234.pdf
BM559 BioMag® Goat anti-Rabbit IgG 1 50 & 500 PDS 559.pdf SDS MB234.pdf
BM560 BioMag® Goat anti-Rat IgG 1 50 & 500 PDS 560.pdf SDS MB234.pdf
BM548 BioMag® Goat anti-Rat IgG (Fc specific) 5 50 & 500 PDS 548.pdf SDS MB233.pdf
BM557 BioMag® Goat anti-Rat IgM 5 50 & 500 PDS 557.pdf SDS MB233.pdf

BioMag® Lectin

Our ConA- and WGA coated BioMag®Plus microparticles provide a convenient means for isolating glycoproteins from serum or cell lysate, and for investigating other lectin/glycan-mediated processes. The BioMag®Plus magnetic particle format provides high surface area, and permits easy and efficient separations.

Catalog Number Description Concentration (mg/mL) Volume (mL) Product Data Sheet Safety Data Sheet
BP530 BioMag®Plus Wheat Germ Agglutinin 5 3, 10 PDS 716.pdf SDS MB233.pdf
BP531 BioMag® Plus Concanavalin A 5 3, 10 PDS 720.pdf SDS MB237.pdf