Fluorescence intensity standards have many applications both in instrument QC programs and in biological analyses. We offer two intensity standard kits for these applications. Our Dragon Green and Flash Red Intensity Standard Kits consist of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of their respective fluorophore.

The different intensity populations may serve as relative intensity standards for applications in fluorescence microscopy, where, as internally-dyed beads, they will stand up to the rigors of imaging. The beads may also serve as bright relative intensity or linearity standards for flow cytometry; ask about our QuickCal® linearity template if this is your interest.

Dragon Green Intensity Standard

The Dragon Green Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets. View excitation and emission spectra here.

Catalog Number Description Product Data Sheet Safety Data Sheet
DG06M Dragon Green Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

References

Miao, Q, Rahn, Bryant J, R, Lancaster, C, Tourovskaia, A , Neumann, T ,… Nelson, A  (2009) Multimodal three-dimensional imaging with isotropic high resolution using optical projection tomography. Proc. SPIE 7262, Medical Imaging : Biomedical Applications in Molecular, Structural, and Functional Imaging

Choi H, Kim KB, Jun C, Chung TD, Kim HC (2012) A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods. Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85484A

Mao X, Lin SS, Dong C, Huang T (2009) Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. Lab Chip, 2009,9, 1583-1589

Flash Red Intensity Standard

The Flash Red Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard. View excitation and emission spectra here.

Catalog Number Description Product Data Sheet Safety Data Sheet
FR06M Flash Red Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

References

Meineke, G, Flitsch, D , Lenenbach, A , Noll, R  (2013) Fluorescence sensors for parallel measurements in multichannel microfluidic devices covering the full channel cross sections. Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI