Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications, Bangs stocks an extensive array of fluorescent microspheres.

Fluorescence intensity standards

Fluorescence intensity standards have many applications both in instrument QC programs and in biological analyses. We offer two intensity standards for these applications. Both the Dragon Green and Flash Red Intensity Kits consist of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of their respective fluorophore.

The different intensity populations may serve as relative intensity standards for applications in fluorescence microscopy, where, as internally-dyed beads, they will stand up to the rigors of imaging. The beads may also serve as bright relative intensity or linearity standards for flow cytometry; ask about our QuickCal® linearity template if this is your interest.

Dragon Green Intensity Standard

The Dragon Green Intensity Standard is a kit consisting of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets.

Catalog Code Description Product Data Sheet Safety Data Sheet
DG06M Dragon Green Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Flash Red Intensity Standard

The Flash Red Intensity Standard is a kit consisting of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard.

Catalog Code Description Product Data Sheet Safety Data Sheet
FR06M Flash Red Intensity Standard (5 Intensities) PDS 704.pdf SDS FG006.pdf

Fluorescent Polymer

Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Diameter (µm) Product Data Sheet
FS02F ≤ 0.49 PDS 731. pdf
FS03F 0.50 - 0.99 PDS 731. pdf
FS04F 1.00 - 1.99 PDS 731. pdf
FS05F 2.00 - 4.99 PDS 731. pdf
FS06F 5.00 - 9.99 PDS 731. pdf
FS07F 10.00 - 24.99 PDS 731. pdf
FS08F ≥ 25.00 PDS 731. pdf

Fluorescent Carboxyl Polymer

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. See spectra for available fluorescent dyes. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Diameter (µm) Product Data Sheet
FC02F ≤ 0.49 PDS 731.pdf  & PDS 741.pdf
FC03F 0.50 - 0.99 PDS 731.pdf
FC04F 1.00 - 1.99 PDS 731.pdf
FC05F 2.00 - 4.99 PDS 731.pdf
FC06F 5.00 - 9.99 PDS 731.pdf
FC07F ≥ 10.00 PDS 731.pdf

Fluorescent Magnetic Polymer

Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the bead matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres are highly uniform and spherical. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code Description Diameter (µm) Product Data Sheet
MC03F Classical Magnetic Polymer 0.50 - 0.99 PDS 731.pdf
ME03F Encapsulated Magnetic Polymer 0.50 - 0.99 PDS 731.pdf
ME04F Encapsulated Magnetic Polymer 1.00 - 1.99 PDS 731.pdf
UMC3F COMPEL™ Magnetic COOH modified, fluorescent 3.00, 6.00

PDS 705.pdf

UMC4F COMPEL™ Magnetic COOH modified, fluorescent 8.00

PDS 705.pdf

Fluorescent Protein Coated Polymer

Our inventory includes a variety of streptavidin coated fluorescent microspheres. Please select the catalog code link below or contact our Customer Service Department for specific availability.

Catalog Code Description Product Data Sheet
CP01F Fluorescent Protein Coated Polymer PDS 721.pdf