Fluorescent microspheres are a mainstay in diagnostics and life sciences research, finding use as fluorescent markers, assay substrates, and instrument standards. In order to meet the unique performance criteria required of microparticles in these different applications, Bangs stocks an extensive array of fluorescent microspheres.
Fluorescence intensity standards
Fluorescence intensity standards have many applications both in instrument QC programs and in biological analyses. We offer two intensity standards for these applications. Both the Dragon Green and Flash Red Intensity Kits consist of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of their respective fluorophore.
The different intensity populations may serve as relative intensity standards for applications in fluorescence microscopy, where, as internally-dyed beads, they will stand up to the rigors of imaging. The beads may also serve as bright relative intensity or linearity standards for flow cytometry; ask about our QuickCal® linearity template if this is your interest.
Dragon Green Intensity Standard
The Dragon Green Intensity Standard is a kit consisting of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets.
Catalog Number | Description | Product Data Sheet | Safety Data Sheet |
---|---|---|---|
DG06M | Dragon Green Intensity Standard (5 Intensities) | PDS 704.pdf | SDS FG006.pdf |
Flash Red Intensity Standard
The Flash Red Intensity Standard is a kit consisting of five populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard.
Catalog Number | Description | Product Data Sheet | Safety Data Sheet |
---|---|---|---|
FR06M | Flash Red Intensity Standard (5 Intensities) | PDS 704.pdf | SDS FG006.pdf |
Fluorescent Polymer
Our non-functionalized microspheres are suitable for coating via adsorption. See spectra for available fluorescent dyes. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.
Catalog Number | Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet |
---|---|---|---|---|
FSDG001 | Dragon Green | 0.05µm | 0.040 - 0.060µm | PDS 731.pdf |
FSFR001 | Flash Red | 0.05µm | 0.040 - 0.060µm | PDS 731.pdf |
FSDG002 | Dragon Green | 0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FSSY002 | Suncoast Yellow | 0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FSFR002 | Flash Red | 0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FSDG003 | Dragon Green | 0.50µm | 0.470 - 0.530µm | PDS 731.pdf |
FSPP003 | Plum Purple | 0.50µm | 0.470 - 0.530µm | PDS 731.pdf |
FSFR003 | Flash Red | 0.50µm | 0.470 - 0.530µm | PDS 731.pdf |
FSDG004 | Dragon Green | 1.00µm | 0.95 - 1.05µm | PDS 731.pdf |
FSEG004 | Envy Green | 1.00µm | 0.95 - 1.05µm | PDS 731.pdf |
FSPP004 | Plum Purple | 1.00µm | 0.95 - 1.05µm | PDS 731.pdf |
FSFR004 | Flash Red | 1.00µm | 0.95 - 1.05µm | PDS 731.pdf |
FSDG005 | Dragon Green | 2.00µm | 1.80 - 2.20µm | PDS 731.pdf |
FSPP005 | Plum Purple | 2.00µm | 1.80 - 2.20µm | PDS 731.pdf |
FSFR005 | Flash Red | 2.00µm | 1.80 - 2.20µm | PDS 731.pdf |
FSDG006 | Dragon Green | 4.00µm | 3.80 - 4.20µm | PDS 731.pdf |
FSEG006 | Envy Green | 4.00µm | 3.80 - 4.20µm | PDS 731.pdf |
FSFR006 | Flash Red | 4.00µm | 3.80 - 4.20µm | PDS 731.pdf |
FSDG007 | Dragon Green | 7.5µm | 7.30 - 7.70µm | PDS 731.pdf |
FSSY007 | Suncoast Yellow | 7.5µm | 7.30 - 7.70µm | PDS 731.pdf |
FSFR007 | Flash Red | 7.5µm | 7.30 - 7.70µm | PDS 731.pdf |
FSEG008 | Envy Green | 10.0µm | 9.50 - 10.50µm | PDS 731.pdf |
FSDG009 | Dragon Green | 15.00µm | 14.5 - 15.50µm | PDS 731.pdf |
FSDG011 | Dragon Green | >25.00µm | >25.00µm | PDS 731.pdf |
Fluorescent Carboxyl Polymer
Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. See spectra for available fluorescent dyes. A general coupling protocol is provided in TechNote 205, Covalent Coupling. Fluorescent carboxyl microspheres and Europium chelate microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.
Catalog Number | Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet |
---|---|---|---|---|
FCDG001 | Dragon Green | 0.05µm | 0.040 - 0.060µm | PDS 731.pdf |
FCFR001 | Flash Red | 0.05µm | 0.040 - 0.060µm | PDS 731.pdf |
FCDG002 | Dragon Green | 0.10µm | 0.090 - 0.110µm | PDS 731.pdf |
FCDG003 | Dragon Green | 0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FCSG003 |
Surf Green |
0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FCFR003 | Flash Red | 0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FCGB003 | Glacial Blue | 0.20µm | 0.190 - 0.210µm | PDS 731.pdf |
FCDG004 | Dragon Green | 0.40µm | 0.370 -0.430µm | PDS 731.pdf |
FCFR004 | Flash Red | 0.40µm | 0.370 -0.430µm | PDS 731.pdf |
FCDG005 | Dragon Green | 0.50µm | 0.470 - 0.530µm | PDS 731.pdf |
FCFR005 | Flash Red | 0.50µm | 0.470 - 0.530µm | PDS 731.pdf |
FCDG006 | Dragon Green | 1.0µm | 0.95 - 1.05µm | PDS 731.pdf |
FCEG006 | Envy Green | 1.0µm | 0.95 - 1.05µm | PDS 731.pdf |
FCSY006 | Suncoast Yellow | 1.0µm | 0.95 - 1.05µm | PDS 731.pdf |
FCGB006 | Glacial Blue | 1.0µm | 0.95 - 1.05µm | PDS 731.pdf |
FCFR006 | Flash Red | 1.0µm | 0.95 - 1.05µm | PDS 731.pdf |
FCSY007 | Suncoast Yellow | 2.0µm | 1.80 - 2.20µm | PDS 731.pdf |
FCDG008 | Dragon Green | 5.0µm | 4.80 - 5.20µm | PDS 731.pdf |
FCEG008 | Envy Green | 5.0µm | 4.80 - 5.20µm | PDS 731.pdf |
FCGB008 | Glacial Blue | 5.0µm | 4.80 - 5.20µm | PDS 731.pdf |
FCFR008 | Flash Red | 5.0µm | 4.80 - 5.20µm | PDS 731.pdf |
FCDG009 | Dragon Green | 10.0µm | 9.50 - 10.50µm | PDS 731.pdf |
FCDG011 | Dragon Green | 15µm | 14.50 - 15.50µm | PDS 731.pdf |
Fluorescent Magnetic Polymer
Both Encapsulated (ME catalog codes) and Classical (MC catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the bead matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres are highly uniform and spherical. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.
Catalog Number | Description | Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet | Safety Data Sheet |
---|---|---|---|---|---|---|
MCDG001 | Classical Magnetic Polymer | Dragon Green | 0.9µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MCFR001 | Classical Magnetic Polymer | Flash Red | 0.9µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEDG002 | Encapsulated Magnetic Polymer | Dragon Green | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MESY002 | Encapsulated Magnetic Polymer | Suncoast Yellow | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEGB002 | Encapsulated Magnetic Polymer | Glacial Blue | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
MEFR002 | Encapsulated Magnetic Polymer | Flash Red | 1.63µm | 0.50 - 2.00µm | PDS 731.pdf | SDS MF220.pdf |
UMGB001 | COMPEL™ Magnetic COOH modified | Glacial Blue | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMDG001 | COMPEL™ Magnetic COOH modified | Dragon Green | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMEG001 | COMPEL™ Magnetic COOH modified | Envy Green | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMFR001 | COMPEL™ Magnetic COOH modified | Flash Red | 3µm | 2.50 - 3.50µm | PDS 705.pdf | SDS MF221.pdf |
UMGB002 | COMPEL™ Magnetic COOH modified | Glacial Blue | 6µm | 5.50 - 6.50µm | PDS 705.pdf | SDS MF221.pdf |
UMDG002 | COMPEL™ Magnetic COOH modified | Dragon Green | 6µm | 5.50 - 6.50µm | PDS 705.pdf | SDS MF221.pdf |
UMFR002 | COMPEL™ Magnetic COOH modified | Flash Red | 6µm | 5.50 - 6.50µm | PDS 705.pdf | SDS MF221.pdf |
UMGB003 | COMPEL™ Magnetic COOH modified | Glacial Blue | 8µm | 7.50 - 8.50µm | PDS 705.pdf | SDS MF221.pdf |
UMDG003 | COMPEL™ Magnetic COOH modified | Dragon Green | 8µm | 7.50 - 8.50µm | PDS 705.pdf | SDS MF221.pdf |
UMFR003 | COMPEL™ Magnetic COOH modified | Flash Red | 8µm | 7.50 - 8.50µm | PDS 705.pdf | SDS MF221.pdf |
Fluorescent Protein Coated Polymer
Our inventory includes a variety of streptavidin coated fluorescent microspheres. Please select the catalog code link below or contact our Customer Service Department for specific availability.
Catalog Number | Coating / Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet |
---|---|---|---|---|
CFDG001 | SA / Dragon Green | 0.20µm | 0.190 - 0.210µm | PDS 721.pdf |
CFDG002 | SA / Dragon Green | 0.40µm | 0.370 - 0.430µm | PDS 721.pdf |
CFFR002 | SA / Flash Red | 0.40µm | 0.370 - 0.430µm | PDS 721.pdf |
CFDG003 | SA / Dragon Green | 0.50µm | 0.470 - 0.530µm | PDS 721.pdf |
CFDG004 | SA / Dragon Green | 1.00µm | 0.95 - 1.05µm | PDS 721.pdf |
CFFR004 | SA / Flash Red | 1.00µm | 0.95 - 1.05µm | PDS 721.pdf |