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Fluorophore-labeled microspheres offer a convenient means to check general instrument status and monitor stability over time. When beads are run, the median channel values for pertinent fluorescence detectors are recorded, and data are monitored to identify outliers and trends. This type of check can alert the user to problems with the optical and fluidic systems (e.g. diminishing laser power or obstruction / leakage) and the effect of environmental factors such as temperature, humidity, and vibration on instrument performance.

 

 

Quantum™ QC, 8 peak beads

Quantum™ QC is an 8 peak multi-intensity, multi-fluorescent standard that is intended for use as an in-depth tool for daily cytometer QC, and is appropriate for use with all lasers and detectors. It may be used to determine detection thresholds, understand resolution, and assess and track linearity of detectors. It can aid in providing confidence that the system is suitable for use, or alert operators to potential problems before samples are run. Quantum™ QC may also be used for instrument set-up to achieve standardized PMT settings and define the window of analysis for relevant detectors. Quantum™ QC is comprised of a blank and 7 (mixed) intensity populations. Quantum QC is used with a QuickCal-Linearity template that is downloaded from our website with an Access Code provided with the product at the time of shipping.

PDS 725, Quantum™ QC, 8 Peak Beads
PDS 725A, Quantum™ QC, 8 Peak Beads Supplement Histograms
SDS ST335, (Quantum™ QC)

Catalog Number Description
725 Quantum™ QC, 8 Peak Beads

Full Spectrum™

Full Spectrum™ microspheres provide a convenient means for performing the initial daily QC check on 3+ color instruments. These ~7-9µm microspheres are internally labeled with multiple fluorophores for use with common excitation and detection wavelengths within the visible spectrum. Full Spectrum™ are available in three amounts: A (20 tests), B (100 tests), and C (280 tests). 


PDS 885, Full Spectrum™ Multi-Color Fluorescence Reference Standard
SDS ST335, (Quantum™ QC)

Catalog Number Description
885 Full Spectrum™

Right Reference Standard™

Each standard consists of a population of microspheres surface labeled with a single fluorochrome at a high intensity level. The Right Reference Standard™ microspheres approximate the size of human lymphocytes (~7-9µm). With their narrow fluorescence CVs, Right Reference Standard™ products are suitable for alignment purposes. These standards may also be used to QC a specific path of the optical system (laser/filter/PMT), and to establish a unified fluorescence range for a particular detector.

PDS 510, Right Reference Standard™
SDS ST334, (Right Reference Standard™)

Catalog Number Description
512 Right Reference Standard™ Fluorescein High Level
515 Right Reference Standard™ Phycoerthrin High Level
518 Right Reference Standard™ PE-Cy™5 High Level
521 Right Reference Standard™ APC High Level

Fluorescence Reference Standards

Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral
characteristics as labeled cells. They may be used to QC a specific path of the optical system, to optimize filter sets for fluorophores and to establish a test-specific Target Channel Value for instrument set-up. 

Flow Cytometry Fluorophore Reference Chart
PDS 890, Fluorescence Reference Standards
SDS ST331, (Fluorescein Standards)
SDS ST332, (Alexa Fluor® Standard)
SDS ST333, (Pacific Blue™ Standard)
SDS ST334, (Fluorescent Reference Standards)
SDS ST335, (Far-Out Red & Violet Laser Standards)
SDS ST341, (Certified Blank™)

Catalog Number Description
890 Certified Blank™
897 Acridine Orange
886 Alexa Fluor® 488
887 Alexa Fluor® 647
901 Allophycocyanine (APC)
898 Chlorophyll
895 Cy™5
906 DAPI
913 Far-Out Red
891 Fluorescein (FITC),
894 Hoechst 33342
916 Pacific Blue™
908 PE-Cy™5
892 Propidium Iodide
899 R-Phycoerythrin (PE)
905 T.M. Rhodamine
893 Texas Red® (TR)
915 Violet Laser

Time Delay Calibration Standard

Bangs' Time Delay Calibration Standard is intended for use in assessing the delay between blue and red lasers. It features ~6µm microspheres dyed with a fluorophore that is excited with 488nm or 635nm excitation, and exhibits red/far-red emission.

PDS 831, Time Delay Calibration Standard
SDS ST335, (Time Delay Standard)

Catalog Number Description
830 Time Delay Calibration Standard