Though the application of particles and cell counting technology is diverse, there is a common need for particulate standards. We manufacture count standards for both dedicated particle counters and flow cytometers. Enumeration of cells in a sample may be achieved through use of a microsphere count standard. Cell count may be calculated by determining the ratio of fluorescent count beads to unlabeled cells.

Flow Cytometry Absolute Count Standard™


 
Our Flow Cytometry Absolute Count Standard™ is a precisely counted population of microspheres for estimating counts of unlabeled cells via flow cytometry. The microspheres are ~7-9µm in diameter and supplied at ~1e+6 beads/mL. Beads are internally labeled with multiple fluorophores for excitation with common lasers (e.g. 488nm, 633nm) and discrimination from the cell population. By evaluating the ratio of microspheres to cells, the volumetric number of cells may be determined.
Catalog Code Nominal Mean Diameter (µm) Product Data Sheet Safety Data Sheet
580 Flow Cytometry Absolute Count Standard™ PDS 880.pdf SDS ST334.pdf


References

Døsen G, Tenstad E, Nygren MK, Stubberud H, Funderud S, Rian E. (2006) Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis. BMC Immunol. Jun 29;7:13.

Xiong, Z, Oriss, TB, Cavaretta, JP, Rosengart, MR, Lee, JS (2012) Red cell microparticle enumeration: validation of a flow cytometric approach. Vox Sang 103(1)p42–48 (Absolute Count standard; use with our Submicron Kit, Cat. 832)

Borzova, E, Dahinden, C, (2014) The Absolute Basophil Count. Meth Mol Biol V.1192 p87-100