Small Bead Calibration Kits

Interest in exosome and microvesicle analysis has grown rapidly in recent years as another way to explore disease states, cancer progression, and for drug discovery and delivery. Due to their small size (30 – 100nm) however, these particles are difficult to detect using traditional methods, and require extra processing to segregate or characterize from debris commonly found in biological samples. Flow cytometry has emerged as a popular method of analysis and sorting for small particle characterization.

Catalog Number Description Product Data Sheet  Safety Data Sheet
834 Nanobead Calibration Kit - 50nm, 100nm PDS 834.pdf SDS ST335.pdf
832 Submicron Bead Calibration Kit - 0.2µm, 0.5µm, 0.8µm PDS 832.pdf SDS ST335.pdf
833 Micron Bead Calibration Kit - 1.0µm, 3.0µm, 6.0µm PDS 832.pdf SDS ST335.pdf

References

Kong, F, Zhang, L, Wang, H, Yuan, G, Guo, A, Li, Q, & Chen, Z. (2015). Impact of collection, isolation and storage methodology of circulating microvesicles on flow cytometric analysis. Experimental and Therapeutic Medicine, 10, 2093-2101.

Huang S, Hou HW, Kanias T, et al. Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage. Lab on a chip. 2015;15(2):448-458.

Østergaard E, Frandsen PL, Sandberg E, (2015) Determination of freeze damage on HPV vaccines by use of flow cytometry. Biologicals Vol. 43,4:266–273

Venable AS, Williams RR, Haviland DL, McFarlin BK (2014) An analysis of endothelial microparticles as a function of cell surface antibodies and centrifugation techniques. J Immunol Methods, Vol406,117-123

Size Calibration Standards

Our Size Calibration Standards Kit for flow cytometry is comprised of five populations of undyed microspheres in the range of ~4-12µm. Forward scatter channel (FSC) values may be plotted against reported bead diameter to generate a curve that may be used for estimation of the sizes of cells run at the same instrument settings. The Size Calibration Standards Kits are available in two sizes: B (100 tests) and C (280 tests).

Catalog Number Description Product Data Sheet  Safety Data Sheet
829 Size Calibration Standards Kit PDS 829.pdf SDS ST334.pdf

References

Mathew G, Timm Jr. EA, Sotomayor P, Godoy A, Montecinos VP, Smith G, Huss WJ (2009) ABCG2-mediated DyeCycle Violet efflux
defined side population in benign and malignant prostate.
Cell Cycle, 8:7, 1053-1061

Obokata H, Kojima K, Yamato M, Okano T, Tsuneda S, Vacanti C, (2011) The Potential of Stem Cells in Adult Tissues Representative of the Three Germ Layers Tissue Eng Part A 17(5-6):607-15