Suspension arrays for the flow cytometer feature populations of microspheres coated with different ligands for the interrogation of multiple targets within a given sample. Ligand-specific microsphere populations are encoded so that results for corresponding individual targets may be discerned. Encoding may be in the form of microsphere size and/or fluorescence signature, as with our QuantumPlex™ and QuantumPlex™M platforms.

Bangs' QuantumPlex™ platform is an innovative bead platform specifically designed for suspension array applications. The beads may be analyzed using any standard flow cytometer (488nm or 633nm excitation), and do not require the use of specialized software.

 

QuantumPlex™

Our QuantumPlex™ kits provide a general platform for multiplexed assay development on standard flow cytometers. Microsphere populations in 5 - bead kits are encoded with different intensities of Starfire Red™ and the two size kits (4.4 μm, 5.5 μm) may be combined to extend the array. Starfire Red™ emits in the red channel (e.g., PE-Cy™5, APC) with very little carry-over into lower wavelengths, leaving other detectors available for determination of positive binding events via common reporters such as FITC and PE. QuantumPlex™ kits are available with two different surfaces to accommodate the coating strategy of choice: carboxyl (COOH) and streptavidin (SA). Carboxyl populations are at a concentration of ~1x108 beads/mL, while Streptavidin are at a concentration of ~1x106 beads/mL. QuantumPlex™ are available in three sizes: A (1mL), B (5mL), and C (10mL).

Catalog Number Description Product Data Sheet Safety Data Sheet
235 QuantumPlex™ COOH 4.4 µm, 5 populations PDS 235 .pdf SDS FG002.pdf
238 QuantumPlex™ COOH 5.5 µm, 5 populations PDS 235 .pdf SDS FG002.pdf
239 QuantumPlex™ COOH 4.4 µm & 5.5 µm, 10 populations PDS 235 .pdf SDS FG002.pdf
215 QuantumPlex™ Streptavidin 4.4 µm, 5 populations PDS 215.pdf SDS FG003.pdf
218 QuantumPlex™ Streptavidin 5.5 µm, 5 populations PDS 215.pdf SDS FG003.pdf
219 QuantumPlex™ Streptavidin 4.4 µm & 5.5 µm, 10 populations PDS 215.pdf SDS FG003.pdf

References

Moss DM, Montgomery JM, Newland SV, Priest JW, Lammie PJ. (2004) Detection of cryptosporidium antibodies in sera and oral fluids using multiplex bead assay. J Parasitol; 90(2):397-404.  [QuantumPlex COOH]

Yu J, Carvalho Mda G, Beall B, Nahm MH. (2008) A rapid pneumococcal serotyping system based on monoclonal antibodies and PCR. J Med Microbiol; 57(Pt 2):171-8.  [QuantumPlex COOH]

Kellar, KL, Iannone, M (2002) Multiplexed microsphere-based flow cytometric assays. Exp. Hematol , V30, 11,p 1227-1237

Hale M, Lee B, Honaker Y, Leung W, Grier A, Jacobs H…Rawlings D. (2017) Homology-Directed Recombination for Enhanced Engineering of Chimeric Antigen Receptor T Cells. Mol Ther Methods Clin Dev; Vol 4 p192-203

Kellar KL, Mahmutovic AJ, Bandyopadhyay K. (2006) Multiplexed microsphere-based flow cytometric immunoassays. Curr Protoc Cytom. Feb;Chapter 13:Unit13.1.

Dolgushin SA, Odintsova ES, Gerasimenko AY, Tronin AV, Tereshchenko SA.(2015) Preliminary Tests of Multiplex Immunoassay for Detection of TORCH Infections in Human Blood Serum Using Flow Cytometry. Biomed Eng. Vol 49, 2,85-89

Spiro A, Lowe M, Brown D.(2000)A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry. Appl. Environ. Microbiol. Vol 66(10)p4258–4265

QuantumPlex™ SP

QuantumPlex™ SP (Single Population) is useful for the development of simplex flow cytometric assays, or for the optimization of attachment chemistry and assay parameters before transitioning to a multiplexed format. Like QuantumPlex™, they are dyed with Starfire Red™ and are suitable for use on standard flow cytometers. Carboxyl (COOH) and streptavidin surfaces are available. Carboxyl populations are at a concentration of ~1x108 beads/mL, while Streptavidin populations are at a concentration of ~1x106 beads/mL. QuantumPlex™ SP are available in two sizes: A (1mL) or B (3mL).

Catalog Number Description Product Data Sheet Safety Data Sheet
234 QuantumPlex™ SP COOH 4.4 µm PDS 234.pdf SDS FG002.pdf
237 QuantumPlex™ SP COOH 5.5 µm PDS 234.pdf SDS FG002.pdf
214 QuantumPlex™ SP Streptavidin 4.4µm PDS 214.pdf SDS FG003.pdf
217 QuantumPlex™ SP Streptavidin 5.5 µm PDS 214.pdf SDS FG003.pdf

QuantumPlex™M

QuantumPlex™M (Magnetic) kits consist of five populations of highly uniform ~6µm superparamagnetic microspheres encoded with different intensities of Starfire Red™. Like QuantumPlex™, they are suitable for use on standard flow cytometers. Highly efficient separations may be performed using rare earth magnetic separators. Carboxyl (COOH) and streptavidin surfaces are available. Carboxyl populations are at a concentration of ~1x108 beads/mL, while Streptavidin populations are at a concentration of ~1x106 beads/mL. QuantumPlex™M are available in three sizes: A (1mL), B (5mL), and C (10mL).

Catalog Number Description Product Data Sheet Safety Data Sheet
250 QuantumPlex™M COOH, 5 populations PDS 250.pdf SDS FG004.pdf
252 QuantumPlex™M Streptavidin, 5 populations PDS 252.pdf SDS FG005.pdf

References

Yang S, Lien K, Huang K, Lei H, Lee G. (2008) Micro flow cytometry utilizing a magnetic bead-based immunoassay for rapid virus detection. Biosens. Bioelectron Vol 24:4 855-862 (QuantumPlex M Streptavidin)

QuantumPlex™M SP

QuantumPlex™M SP (Magnetic, Single Population) is useful for the development of simplex flow cytometric assays, or for the optimization of attachment chemistry and assay parameters before transitioning to a multiplexed format. Like QuantumPlex™M, they are highly uniform ~6µm superparamagnetic microspheres dyed with Starfire Red™ and are suitable for use on standard flow cytometers. Carboxyl (COOH) and streptavidin surfaces are available. Carboxyl populations are at a concentration of ~1x108 beads/mL, while Streptavidin are at a concentration of ~1x106 beads/mL. QuantumPlex™M SP are available in two sizes: A (1mL) or  B (3mL).

Catalog Number Description Product Data Sheet Safety Data Sheet
251 QuantumPlex™M SP COOH PDS 251.pdf SDS FG004.pdf
253 QuantumPlex™M SP Streptavidin PDS 253.pdf SDS FG005.pdf