Though particle counting technology applications are diverse, there is a common need for instrument validation and ongoing QC. Microsphere-based particle count standards may be used to validate liquid counters across their dynamic ranges and to ensure continued capability through the performance of daily QC checks. The use of a reference material permits the standardization of results between runs, instruments and laboratories, and over time. Our particle count standards include both general and instrument-focused (flow cytometer, cell viability analyzer) controls.
SureCount™ Particle Count Standard
SureCount™ standards are suspensions of polymer microspheres intended for the validation and monitoring of particle counters and supporting sample preparation processes. The diameters of the SureCount™ standards are traceable to NIST Standard Reference Materials. The standards are supplied as ~1 x 106 microspheres/mL aqueous suspensions in 10mL volumes, and each bottle is provided with a Certificate of Traceability.
PDS 733, SureCount™ Particle Count Standards Featuring NIST Traceable Size Standards
TSD 0706, Handling & Pipetting Concentration Standards
SDS ST350, (SureCount™)
Catalog Number | Nominal Mean Diameter (µm) | Volume (mL) |
---|---|---|
CC03N | 3 | 10 |
CC05N | 5 | 10 |
CC10N | 10 | 10 |
CC15N | 15 | 10 |
References
Kim J, Kim E, Bae S, Kwon S, Chun H.(2013) Potentiometric Multichannel Cytometer Microchip for High-throughput Microdispersion Analysis Anal. Chem.85 (1), pp 362–368
Garzio LM, Steinberg DK, Erickson M, & Ducklow HW. (2013). Microzooplankton grazing along the Western Antarctic Peninsula. Aquatic Microbial Ecology, 70(3), 215-232.(Cat. CC10N, SureCount 10µm)
Bekeschus S, Moritz J, Schmidt A, & Wende K. (2017). Redox regulation of leukocyte-derived microparticle release and protein content in response to cold physical plasma-derived oxidants. Clinical Plasma Medicine, 7, 24-35.
Flow Cytometry Absolute Count Standard™
PDS 880, Flow Cytometry Absolute Count Standard™
SDS ST334, (Absolute Count Standard™)
Catalog Number | Nominal Mean Diameter (µm) |
---|---|
580 | Flow Cytometry Absolute Count Standard™ |
References
Heinrich LF, Andersen DK, Cleasby ME, & Lawson C. (2015). Long-term high fat feeding of rats results in increased numbers of circulating microvesicles with pro-inflammatory effects on endothelial cells. British Journal of Nutrition, 113(11), 1704-1711.
ViaCheck™ Concentration Controls
Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Concentration Controls are supplied at the noted concentrations.
PDS 711, ViaCheck™ Concentration Controls
TSD 0706, Handling & Pipetting Concentration Standards
Appl.Note 0708, (Optimization of ViaCheck™)
TSD 0711, ViaCheck™ Concentration Controls
SDS ST353, (ViaCheck™ Concentration)
Catalog Number | Description | Volume (mL) |
---|---|---|
VC50N | ViaCheck™ Concentration Control (0.5 × 106) | 20 |
VC60N | ViaCheck™ Concentration Control (1 × 106) | 20 |
VC70N | ViaCheck™ % Concentration Control (4 × 106) | 20 |
VC80N | ViaCheck™ % Concentration Control (8 × 106) | 20 |
ViaCheck™ Concentration Controls - SingleShots™
ViaCheck™ SingleShots™, affordable single-use QC. Mix, dispense & run for confirmation of live/dead ratios and counts. ViaCheck™ SingleShots™ minimize error and maximize efficiency by circumventing potential human error from repeated bottle resuspension & pipetting steps.
PDS 711, ViaCheck™ Concentration Controls
TSD 0706, Handling & Pipetting Concentration Standards
Appl.Note 0708, (Optimization of ViaCheck™)
TSD 0711, ViaCheck™ Concentration Controls
SDS ST353, (ViaCheck™ Concentration)
Catalog Number | Description | Units |
---|---|---|
VC50NSS | ViaCheck™ Concentration Control (0.5 × 106) SingleShots™ | 25 or 75 |
VC60NSS | ViaCheck™ Concentration Control (1 × 106) SingleShots™ | 25 or 75 |
VC70NSS | ViaCheck™ % Concentration Control (4 × 106) SingleShots™ | 25 or 75 |
VC80NSS | ViaCheck™ % Concentration Control (8 × 106) SingleShots™ | 25 or 75 |