Extracellular Vesicle Analysis By Flow Cytometry

Posted: June 19, 2019

When studying extracellular vesicles, it is helpful to have some tools to guide the operator in their analysis. Particle reference standards assist the investigator by providing confidence in sizing, quantitation and particle count. In continuing with last week’s theme on small particle analysis, we are providing this list of literary and product references. All the products listed have associated primary literature references so you can see how your colleagues employed the kits. Of course, you can modify them for your needs, and we would be happy to help you refine or troubleshoot your assay.

Lannigan, J., P. Nolan, J., & Zucker, R. (2016). Measurement of extracellular vesicles and other submicron size particles by flow cytometry. Cytometry Part A, 89(2), 101-220. (General)

Arraud, N., Gounou, C., Turpin, D., & Brisson, A. R. (2016). Fluorescence triggering: A general strategy for enumerating and phenotyping extracellular vesicles by flow cytometry. Cytometry Part A, 89(2), 184-195.

Erdbrügger, U., La Salvia, S., & Lannigan, J. Detection of Extracellular Vesicles Using the ImageStream® X MKII Imaging Flow Cytometer . (MESF)

Erdbrügger, U., & Lannigan, J. (2016). Analytical challenges of extracellular vesicle detection: a comparison of different techniques. Cytometry Part A, 89(2), 123-134. (MESF, size, count)

Fischer, S., Cornils, K., Speiseder, T., Badbaran, A., Reimer, R., Indenbirken, D., ... & Lange, C. (2016). Indication of horizontal DNA gene transfer by extracellular vesicles. PLoS One, 11(9), e0163665. (Count, size)

Konokhova, A. I., Chernova, D. N., Moskalensky, A. E., Strokotov, D. I., Yurkin, M. A. & Maltsev, V. P. (2016). Super‐resolved calibration‐free flow cytometric characterization of platelets and cell‐derived microparticles in platelet‐rich plasma. Cytometry Part A, 89(2), 159-168. (General)

Kormelink, T. G., Arkesteijn, G. J., Nauwelaers, F. A., van den Engh, G., Nolte‐’t Hoen, E. N., & Wauben, M. H. (2016). Prerequisites for the analysis and sorting of extracellular vesicle subpopulations by high‐resolution flow cytometry. Cytometry Part A, 89(2), 135-147. (Size)

Lannigan, J., & Erdbruegger, U. (2017). Imaging flow cytometry for the characterization of extracellular vesicles. Methods, 112, 55-67. (MESF)

Mobarrez, F., Antovic, J., Egberg, N., Hansson, M., Jörneskog, G., Hultenby, K., & Wallén, H. (2010). A multicolor flow cytometric assay for measurement of platelet-derived microparticles. Thrombosis research, 125(3), e110-e116. (MESF)

Mooberry, M. J., & Key, N. S. (2016). Microparticle analysis in disorders of hemostasis and thrombosis. Cytometry Part A, 89(2), 111-122. (General)

Morales-Kastresana, A., Telford, B., Musich, T. A., McKinnon, K., Clayborne, C., Braig, Z., ... & Freeman, G. J. (2017). Labeling extracellular vesicles for nanoscale flow cytometry. Scientific reports, 7(1), 1878. (MESF)

Nolan, J. P., & Jones, J. C. (2017). Detection of platelet vesicles by flow cytometry. Platelets, 28(3), 256-262. (General)