Do you have tools for assessing receptor occupancy (RO) in biopharmaceutical development?
Flow cytometry is an important technology for conducting receptor occupancy assays in drug / biotherapeutic development, including free receptor, total receptor, bound receptor and receptor modulation formats. Our Quantum MESF and Quantum Simply Cellular kits permit the quantitation of cellular fluorescence, be it from fluorophore-tagged drug / biotherapeutic agent, noncompetitive antibody, or anti-drug antibody. Quantum kits are available in a broad range of fluorophores and anti-species antibodies for calibration of binding events in standardized MESF (Molecules of Equivalent Soluble Fluorochrome) or ABC (Antibody Binding Capacity) units. See the following references for specific examples of kit use and flow cytometry methods:
Quadrini KJ, Hegelund AC, Cortes KE, Xue C, Kennelly SM, Ji H, Högerkorp C-M, Mc Closky TW. (2016) Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug development. Cytometry B Clin Cytom; 90B:177-190.
Sternebring O, Alifrangis L, Christensen TF, Ji H, Hegelund AC, Högerkorp CM. (2016) A weighted method for estimation of receptor occupancy for pharmacodynamic measurements in drug development. Cytometry B Clin Cytom. Mar;90(2):220-9
Stewart JJ, Green CL, Jones N, Liang M, Xu Y, Wilkins DE,…Litwin V. (2016) Role of receptor occupancy assays by flow cytometry in drug development. Cytometry B Clin Cytom. Mar;90(2):110-6
Sklar LA, Finney DA.(1982) Analysis of ligand-receptor interactions with the fluorescence activated cell sorter. Cytometry Nov;3(3):161-5.