Product-Specific TechNotes (100 Series)

100: Polymer Microspheres
Handling tips and guidelines for working with polymer microspheres. (01/10)

101: ProActive® Microspheres
Handling tips and protocols for streptavidin, Protein A, and anti-Mouse coated microspheres. (04/08)

102: Magnetic Microparticles
Magnetic particle applications, with product suggestions. (08/10)

102A: Magnetic Particle Product Lines
Overview of Bangs' magnetic particle products, including ProMag™, BioMag®, and COMPEL™. (01/11)

103: Fluorescent / Dyed Microspheres
Applications, fluorescence spectra, and product descriptions. Includes QuantumPlex™ microspheres for multiplexing and flow cytometry, and confocal microscope standards. (08/11)

104: Silica Microspheres
For immunoassays, nucleic acid capture, velocimetry (LDV, PIV), flat panel display spacers, and others. Tips for drying and resuspension. (04/08)

105: Microsphere Size Standards
Beads for cell size estimation, filter challenge, and instrument checks and calibrations. NIST-traceable standards from 0.27µm to 25µm. (09/07)

106: Confocal Standards
Using fluorescent bead standards in confocal microscopy. (08/07)

Handling-Specific TechNotes (200 Series)

201: Working with Microspheres
Choosing, cleaning, characterizing, coating beads, etc. (04/08)

201A: Microsphere Selection
Description of properties that impact base bead selection. (10/08)

202: Microsphere Aggregation
Preventing, detecting, and reversing aggregation. Chemicals and equipment sources. (04/09)

203: Washing Microspheres
Protocols for different methods for cleaning microspheres; advantages/disadvantages of methods; suppliers of equipment. (04/08)

203A: Drying & Re-Suspending Microspheres
Converting aqueous suspensions to dry microspheres and vice versa. (06/13)

204: Adsorption to Microspheres
Adsorbing proteins onto particles; use of "surface diluents" (blockers); recipes and references. (04/08)

205: Covalent Coupling
Chemical attachment of proteins, nucleic acids, etc. to functionalized microspheres. Suggestions for coupling protocol design and optimization (buffer and blocker selection, use of chemical linkers, etc.). Recommended reading and references. (04/08)

206: Equations
For calculating particles/mL, surface area/g, "parking area," settling velocity @ 1G and in centrifuge, etc. (10/12)

208: Microsphere Sizing
BLI methods are described and discussed, along with Optical Microscopy and other methods. (08/09)

Application-Specific TechNotes (300 Series)

301: Immunological Applications
Review of commercial applications of microspheres. (04/08)

302: Molecular Biology
Overview of purification and solid phase separation methods. (04/08)

303: Lateral Flow Tests
Putting dyed particles on membranes, so they will move properly. (04/08)

304: Light-Scattering Assays
Turbidimetric and nephelometric applications of microspheres. (04/08)

Reprints (400 Series)

402: Microspheres, part 1: Selection, cleaning, and characterization, and part 2: Ligand attachment and test formulation. Leigh B. Bangs & Mary Meza. IVD Technology (in Medical Device & Diagnostic Industry), 17, #3, 18-26, March, and #4, 20-26, April, 1995. Good general description of how to work with microspheres: test component selection, cleaning, characterization, adsorption, covalent attachment, coating optimization, final formulation, filter interaction, and strip test assembly.

403: New Developments in Particle-Based Immunoassays: Introduction L.B. Bangs. Pure & Appl. Chem., 68, #10, 1873-1879 (1996). Review of 40 years of uses of microspheres in diagnostic tests and assays from LATS to biosensors. (Invited paper from the 6th International Congress on Automation and New Technology in the Clinical Laboratory, Barcelona, November 4, 1995.)

405: Applications of Magnetic Particles in Immunoassays Mary Meza. Ch. 22 (pp. 303-309) in "Scientific and Clinical Applications of Magnetic Carriers," ed-by Häfeli, et al. Plenum Press, New York, 1997. Lecture at conference in Rostock, Germany, September 1996.

406: Measuring Microspheres Binding Capacity J.M. Duffy, J.V. Wall, M.B. Meza, L.J. Jenski. IVD Technology, 4, #7, 28-34 (1998).

407: Bead-based HTS applications in drug discovery M.B. Meza. Drug Discovery Today: HTS supplement, 1, #1, 38-41 (2000).