Bangs Laboratories, Inc.


	Newsletters \| Ask the Doc \| References \| TechNotes \| Links Fluorescent Dye Spectra \| Visible Dye Palette \| Recent Presentations \| FAQs

05 July 2008 4:53 AM EDT

Product Specific | Handling Specific | Application Specific | Reprints | Presentations

All our technical resources are made available as Adobe Acrobat (PDF) files. In order to view and/or print these files, please make sure you have version 5.0 or later of Adobe Acrobat Reader. It's a free download from Adobe.com.

101	ProActive® Microspheres
	Handling tips + protocols for streptavidin, Protein A, and anti-Mouse coated microspheres. (08/99)

102	Magnetic Microparticles
	Magnetic particle applications, with product suggestions. (01/04)

103	Fluorescent/Dyed Microspheres
	Applications, fluorescence spectra, and product descriptions. Includes QuantumPlex™ microspheres for multiplexing and flow cytometry and confocal microscope standards. (01/03)

104	Silica Microspheres
	For immunoassays, nucleic acid capture, velocimetry (LDV, PIV), flat panel display spacers, others. Tips for drying and resuspension. (08/99)

105	Microsphere Size Standards
	Beads for cell size estimation, filter challenge, and instrument checks and calibrations. NIST-traceable standards from 0.27µm to 25µm. (01/03)

106	Confocal Standards
	Using fluorescent bead standards in confocal microscopy. (08/07)

201	Working with Microspheres
	Choosing, cleaning, characterizing, coating beads, etc. (L.B. Bangs 2nd lecture at The Latex Course™ - 10/02)

202	Microsphere Aggregation
	Preventing, detecting, and reversing aggregation. Chemicals and equipment sources. (08/99)

203	Washing Microspheres
	Protocols for different methods for cleaning microspheres; advantages/disadvantages of methods; suppliers of equipment. (08/99)

204	Adsorption to Microspheres
	Adsorbing proteins onto particles; use of "surface diluents" (blockers); recipes and references. (08/99)

205	Covalent Coupling
	Chemical attachment of proteins, nucleic acids, etc. to functionalized microspheres. Suggestions for coupling protocol design and optimization (buffer and blocker selection, use of chemical linkers, etc.). Vendor information, recommended reading and references. (03/02)

206	Equations
	For calculating particles/mL, surface area/g, "parking area," settling velocity @ 1G and in centrifuge, etc. (10/01)

208	Microsphere Sizing
	BLI methods are described and discussed, along with Optical Microscopy and other methods. (08/03)

301	Immunological Applications
	Review of commercial applications of microspheres. (L.B. Bangs 1st lecture at The Latex Course™ - 05/99)

302	Molecular Biology
	Overview of purification and solid phase separation methods. (02/02)

303	Lateral Flow Tests
	Putting dyed particles on membranes so they will move properly. (08/99)

304	Light-Scattering Assays
	Turbidimetric and nephelometric applications of microspheres. (08/99)

402	Microspheres, part 1: Selection, cleaning, and characterization, and part 2: Ligand attachment and test formulation.
	Leigh B. Bangs & Mary Meza IVD Technology (in Medical Device & Diagnostic Industry), 17, #3, 18-26, March, and #4, 20-26, April, 1995. Good general description of how to work with microspheres: test component selection, cleaning, characterization, adsorption, covalent attachment, coating optimization, final formulation, filter interaction, and strip test assembly.
	These may be viewed directly on the publisher's website at http://www.devicelink.com/ivdt/archive/95/03/009.html and http://www.devicelink.com/ivdt/archive/95/04/006.html.

403	New Developments in Particle-Based Immunoassays: Introduction
	L.B. Bangs Pure & Appl. Chem., 68, #10, 1873-1879 (1996). Review of 40 years of uses of microspheres in diagnostic tests and assays from LATS to biosensors. (Invited paper from 6th Int'l Congress on Automation and New Technology in the Clinical Laboratory, Barcelona, November 4, 1995.)

405	Applications of Magnetic Particles in Immunoassays
	Mary Meza. Ch.22 (pp.303-309) in Scientific and Clinical Applications of Magnetic Carriers ed-by H�feli, et al. Plenum Press, New York,1997, Lecture at conference in Rostock, Germany, September 1996.

406	Measuring Microspheres Binding Capacity
	J.M. Duffy, J.V. Wall, M.B. Meza, L.J. Jenski, IVD Technology, 4, #7, 28-34 (1998).

407	Bead-based HTS applications in drug discovery
	M.B. Meza, Drug Discovery Today: HTS supplement, 1, #1, 38-41 (2000).

	QuantumPlex™ Beads Provide the Ideal Platform for a Variety of Multiplexing Applications
	N. Foushee, AACC, OEM Lecture Series, Chicago, IL, August 2001.

	Microsphere Characteristics for Rapid Test Development
	M. Meza, Diagnostic Support 2001, Chicago, IL, April 24-25, 2001.

	ProActive® Streptavidin Coated Microspheres and Their Binding Capacity for Biotin and Biotinylated Oligonucleotides
	K. Turner, AACC, OEM Lecture Series, San Francisco, CA, July 25-26, 2000.

Bangs Laboratories, Inc. is a supplier of uniform polymeric and silica microsphere products
for diagnostic, research, and flow cytometry applications. BLI's newest QuantumPlex flow cytometry
multiplexing kits complement specialty microspheres for a variety of immunoassay, molecular
and cell biology and standardization applications.