Functionalized Polymer

Our functionalized polystyrene (PS) and poly(methyl methacrylate) (PMMA) microspheres are suitable for the covalent immobilization of proteins, peptides, and nucleic acids. They are synthesized via emulsion polymerization, and are available in diameters up to ~20µm, with typical size CVs of 10%. Most products are supplied as 10% solids suspension (w/w) in de-ionized water with surfactant and sodium azide.

Carboxyl Polymer

EDAC-mediated coupling is often used for the covalent immobilization of amine-terminated proteins, DNA, or other molecules on carboxyl-functionalized PS and P(S/DVB) microspheres. See TechNote 205, Covalent Coupling, for a protocol and tips on coating optimization and QC. Carboxyl polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available. 
Catalog Number Catalog Code Nominal Diameter

Specification Range

Product Data Sheet
PC02001 PC02N 0.025µm 0.015 - 0.035µm PDS 732.pdf
PC02002 PC02N 0.050µm 0.040 - 0.060µm PDS 732.pdf
PC02003 PC02N 0.070µm 0.061 - 0.080µm PDS 732.pdf
PC02004 PC02N 0.100µm 0.090 - 0.110µm PDS 732.pdf
PC02005 PC02N 0.125µm 0.115 - 0.135µm PDS 732.pdf
PC02006 PC02N 0.150µm 0.140 - 0.160µm PDS 732.pdf
PC02007 PC02N 0.175µm 0.165 - 0.185µm PDS 732.pdf
PC02008 PC02N 0.200µm 0.190-0.210µm PDS 732.pdf
PC02009 PC02N 0.300µm 0.270-0.330µm PDS 732.pdf
PC02010 PC02N 0.350µm 0.340 - 0.360µm PDS 732.pdf
PC02011 PC02N 0.400µm 0.370 - 0.430µm PDS 732.pdf
PC03001 PC03N 0.500µm 0.470 - 0.530µm PDS 732.pdf
PC03002 PC03N 0.600µm 0.570 - 0.630µm PDS 732.pdf
PC03003 PC03N 0.800µm 0.770 - 0.830µm PDS 732.pdf
PC03004 PC03N 0.900µm 0.870 - 0.930µm PDS 732.pdf
PC04001 PC04N 1.0µm 0.95 - 1.05µm PDS 732.pdf
PC05001 PC05N 2.00µm 1.80 - 2.20µm PDS 732.pdf
PC05002 PC05N 2.50µm 2.30 - 2.70µm PDS 732.pdf
PC05003 PC05N 3.00µm 2.80 - 3.20µm PDS 732.pdf
PC05004 PC05N 4.00µm 3.80 - 4.20µm PDS 732.pdf
PC05005 PC05N 4.50µm 4.30 - 4.70µm PDS 732.pdf
PC06001 PC06N 5.00µm 4.80 - 5.20µm PDS 732.pdf
PC06002 PC06N 5.50µm 5.30 - 5.70µm PDS 732.pdf
PC06003 PC06N 6.00µm 5.80 - 6.20µm PDS 732.pdf
PC06004 PC06N 7.00µm 6.80 - 7.20µm PDS 732.pdf
PC07001 PC07N 10.00µm 9.50 - 10.50µm PDS 732.pdf
PC07002 PC07N 15.00µm 14.50µm - 15.50µm PDS 732.pdf
PC07003 PC07N 20.00µm 19.00µm - 21.00µm PDS 732.pdf
PC08001 PC08N >25.µm >25.00µm PDS 732.pdf

References

Park, MK, Briles, DE, Nahm, MH (2000) A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay) Clin Vaccine Immunol v7(3) 486-489

Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced Cellulose Degradation Using Cellulase-Nanosphere Complexes. PLoS ONE 7(8): e42116

Rodrigues OR, & Monard S, (2016) A rapid method to verify single-cell deposition setup for cell sorters. Cytometry 89: 594–600

Borque L, Bellod L, Rus A, Seco L, Galisteo-Gonzàles F (2000) Development and Validation of an Automated and Ultrasensitive Immunoturbidimetric Assay for C-Reactive Protein. Clin Chem 46(11) 1839-1842

Perez-Amodio S, Holownia P, Davey C, Price C (2001) Effects of the Ionic Environment, Charge, and Particle Surface Chemistry for Enhancing a Latex Homogeneous Immunoassay of C-Reactive Protein. Anal. Chem.73 (14) 3417–3425

Lucas L, Han J, Yoon J (2006) Using highly carboxylated microspheres to simplify immunoassays and enhance diffusional mixing in a microfluidic device. Colloids and Surf. B: Biointerfaces 49(2) 106-111

Fu L, Kang C, Zhang P, Kang X, Li X, Wang H, …Qiu Y (2015) A modified quick PETIA for detecting anti-CCP antibodies in human serum, Anal Methods Issue 7

Deegan O, Walshe K, Kavanagh K, Doyle S. (2003) Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres. Anal. Biochem. 312 p175–181 (turbidimetric assay, 0.21µm PS-COOH)

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Amine Polymer

Amine-functionalized microspheres offer further options for coupling of biomolecules. Glutaraldehyde is commonly used for the immobilization of amine-containing compounds, and specialized linkers are available for targeted coupling of thiol or carbohydrate moieties to amine-functionalized microspheres. See TechNote 205, Covalent Coupling. Unless otherwise noted amine polymer microspheres are availableas ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g. For microspheres larger than 1.99µm, please call for availability.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available. 
Catalog Number Catalog Code Nominal Diameter

Specification Range

Product Data Sheet
PA02001 PA02N 0.200µm 0.190 - 0.210µm PDS 732.pdf
PA03001 PA03N 0.500µm 0.470 - 0.530µm PDS 732.pdf
PA03002 PA03N 0.750µm 0.720 - 0.780µm PDS 732.pdf
PA04001 PA04N 1.0µm 0.95 - 1.05µm PDS 732.pdf

References

Schleicher KD, Dettmer SL, Kapinos LE, Pagliara S, Keyser UF, Jeney S, LimRoderick YH (2014) Selective transport control on molecular velcro made from intrinsically disordered proteins. Nat Nano 9, 525-530

Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, Lou X… Tanner S.(2009) Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry. Anal Chem 81,6813–6822

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