Our functionalized polystyrene (PS) and poly(methyl methacrylate) (PMMA) microspheres are suitable for the covalent immobilization of proteins, peptides, and nucleic acids. They are synthesized via emulsion polymerization, and are available in diameters up to ~20µm, with typical size CVs of 10%. Most products are supplied as 10% solids suspension (w/w) in de-ionized water with surfactant and sodium azide.
EDAC-mediated coupling is often used for the covalent immobilization of amine-terminated proteins, DNA, or other molecules on carboxyl-functionalized PS and P(S/DVB) microspheres. See TechNote 205, Covalent Coupling, for a protocol and tips on coating optimization and QC. Carboxyl polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.
|PC03N||0.50 - 0.99|
|PC04N||1.00 - 1.99|
|PC05N||2.00 - 4.99|
|PC06N||5.00 - 9.99|
Park, MK, Briles, DE, Nahm, MH (2000) A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay) Clin Vaccine Immunol v7(3) 486-489
Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced Cellulose Degradation Using Cellulase-Nanosphere Complexes. PLoS ONE 7(8): e42116
Rodrigues OR, & Monard S, (2016) A rapid method to verify single-cell deposition setup for cell sorters. Cytometry 89: 594–600
Borque L, Bellod L, Rus A, Seco L, Galisteo-Gonzàles F (2000) Development and Validation of an Automated and Ultrasensitive Immunoturbidimetric Assay for C-Reactive Protein. Clin Chem 46(11) 1839-1842
Perez-Amodio S, Holownia P, Davey C, Price C (2001) Effects of the Ionic Environment, Charge, and Particle Surface Chemistry for Enhancing a Latex Homogeneous Immunoassay of C-Reactive Protein. Anal. Chem.73 (14) 3417–3425
Lucas L, Han J, Yoon J (2006) Using highly carboxylated microspheres to simplify immunoassays and enhance diffusional mixing in a microfluidic device. Colloids and Surf. B: Biointerfaces 49(2) 106-111
Amine-functionalized microspheres offer further options for coupling of biomolecules. Glutaraldehyde is commonly used for the immobilization of amine-containing compounds, and specialized linkers are available for targeted coupling of thiol or carbohydrate moieties to amine-functionalized microspheres. See TechNote 205, Covalent Coupling. Unless otherwise noted amine polymer microspheres are availableas ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g. For microspheres larger than 1.99µm, please call for availability.
|PA03N||0.50 - 0.99|
|PA04N||1.00 - 1.99|
Schleicher KD, Dettmer SL, Kapinos LE, Pagliara S, Keyser UF, Jeney S, LimRoderick YH (2014) Selective transport control on molecular velcro made from intrinsically disordered proteins. Nat Nano 9, 525-530
Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, Lou X… Tanner S.(2009) Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry. Anal Chem 81,6813–6822