Affinity Ligand Coated Microspheres

When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of Ab/Ag complexes in immunoprecipitation.

Streptavidin

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, ProActive® Microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

Streptavidin Polymer

Catalog Number Nominal Diameter Specification Range Product Data Sheet
CP01000 0.10µm 0.090 - 0.110µm PDS 721.pdf
CP01001 0.20µm 0.190 - 0.210µm PDS 721.pdf
CP01003 0.50µm 0.470 - 0.530µm PDS 721.pdf
CP01004 1.00µm 0.95 - 1.05µm PDS 721.pdf
CP01005 3.0µm 2.80 - 3.20µm PDS 721.pdf
CP01006 5.00µm 4.80 - 5.20µm PDS 721.pdf
CP01007 10.00µm 9.50 - 10.50µm PDS 721.pdf
CP01008 15.0µm 14.50 - 15.50µm PDS 721.pdf

Streptavidin Magnetic Polymer

The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes.

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
CMC0100 SA / Classical Magnetic Polymer 0.2 - 0.5µm 0.2 - 0.5µm PDS 721.pdf
CME0101 SA / Encapsulated Magnetic Polymer 0.86µm 0.7 - 1.2µm PDS 721.pdf
UMC0100 SA/ COMPEL 3µm 2.50 - 3.50µm PDS 721.pdf
UMC0101 SA / COMPEL 6µm 5.50 - 6.50µm PDS 721.pdf
UMC0102 SA / COMPEL 8µm 7.50 - 8.50µm PDS 721.pdf

 Streptavidin Fluorescent Polymer 

Catalog Number Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
CFDG001 SA / Dragon Green 0.20µm 0.190 - 0.210µm PDS 721.pdf
CFDG002 SA / Dragon Green 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFFR002 SA / Flash Red 0.40µm 0.370 - 0.430µm PDS 721.pdf
CFDG003 SA / Dragon Green 0.50µm 0.470 - 0.530µm PDS 721.pdf
CFDG004 SA / Dragon Green 1.00µm 0.95 - 1.05µm PDS 721.pdf
CFFR004 SA / Flash Red 1.00µm 0.95 - 1.05µm

PDS 721.pdf

Streptavidin Dyed Polymer

Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.

Catalog Number Coating / Dye Nominal Diameter Specification Range
CDCR001 SA / Crimson Red 0.20µm 0.190 - 0.210µm
CDCB001 SA / Cabo Blue 0.20µm 0.190 - 0.210µm

References

Sepp, A, Tawfik, D, Griffiths, D(2002) Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry. FEBS Letters V532(3)P455–458

Kahanovitz L, Seker E, Marks RS, Yarmush ML, Konry T, Russell SJ (2016) Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring. J Diabetes Sci Technol 10(3):689-96

Tanga MYH, Shum HC (2016) One-step immunoassay of C-reactive protein using droplet microfluidics Lab Chip, 16, 4359-4365

CP01F, CP01N, CP01B, CP01R, CP01O, CP01K, CP01V, CP01G, CP01, CM01

Biotin

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See TechNote 101, ProActive® Microspheres, for sample applications and procedures.

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
CP10000 Polymeric / Biotin 10µm 9.50 - 10.50µm PDS 724.pdf

Protein A and Protein G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, ProActive® Microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
CP02000 Polymer / Protein A 1.00µm 0.95-1.05µm PDS 722.pdf
CP02001 Polymer / Protein A 5.00µm 4.80-5.20µm PDS 722.pdf
CP02002 Polymer / Protein A 10.0µm 9.50-10.50µm PDS 722.pdf
PMG3N ProMag™ (Magnetic) / Protein G 3µm 2.70 - 3.30µm PDS 722.pdf
CM02, CP02