Affinity Ligand Coated Microspheres

When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of Ab/Ag complexes in immunoprecipitation.

Streptavidin

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, ProActive® Microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

Catalog Code Description Product Data Sheet
CM01N Magnetic / Streptavidin PDS 721.pdf
CP01N Polymer / Streptavidin PDS 721.pdf
CP01F Fluorescent Polymer / Streptavidin PDS 721.pdf
CP01* Dyed Polymer / Streptavidin PDS 721.pdf

* The final character of the catalog code denotes color: B = Blue, R = Red, O = Orange, K = Black, V = Purple, G = Green, F = Fluorescent, N=None.

References

Sepp, A, Tawfik, D, Griffiths, D(2002) Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry. FEBS Letters V532(3)P455–458

Kahanovitz L, Seker E, Marks RS, Yarmush ML, Konry T, Russell SJ (2016) Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring. J Diabetes Sci Technol 10(3):689-96

Tanga MYH, Shum HC (2016) One-step immunoassay of C-reactive protein using droplet microfluidics Lab Chip, 16, 4359-4365

CP01F, CP01N, CP01B, CP01R, CP01O, CP01K, CP01V, CP01G, CP01, CM01

Biotin

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See TechNote 101, ProActive® Microspheres, for sample applications and procedures.

Catalog Code Description Product Data Sheet
CP10N Polymeric / Biotin PDS 724.pdf

Protein A and Protein G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, ProActive® Microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

Catalog Code Description Product Data Sheet
CM02N Magnetic / Protein A PDS 722.pdf
CP02N Polymer  / Protein A PDS 722.pdf
PMG3N ProMag™ (Magnetic) / Protein G PDS 722.pdf
CM02, CP02