Magnetic Microspheres

Microsphere

Magnetic Microparticles

We couldn't think of a good pun for "magnetic".

Superparamagnetic particles have been utilized extensively in diagnostics and other research applications for the purification of cells and biomolecules, such as antibodies, nucleic acids, and polypeptides. They confer a number of benefits, including ease of separation and suitability for automation. When coated with recognition molecules, magnetic microspheres are ideal for the efficient capture and separation of target. Unwanted sample constituents may be washed away following a simple magnetic separation step.

Our three lines of superparamagnetic microparticles allow us to uniquely address a wide range of applications in the life sciences, from cell separations and immunoassays to suspension arrays and flow cytometry.

ProMag™ and ProMag™ HP

ProMag™ are highly uniform polymer-based magnetic spheres available in diameters of 1µm and 3µm. A unique surface means low nonspecific binding in protein-based systems, and superior handling without the use of surfactant. These high-binding beads are suitable for use across a range of research and diagnostic applications, whether you're working at laboratory scale or have the more stringent requirements of high throughput applications. 

NEW ProMag™ HP (High Performance) have been meticulously engineered for use in assay development. The highly optimized composition ensures lowest autosignal, particularly with respect to chemiluminescence and exposed iron.

Catalog Code Description Product Data Sheet Material Safety Data Sheet
PMA1NProMag™ 1 Series • AminePDS 715.pdfMSDS ProMag COOH-NH2.pdf
PMB1NProMag™ 1 Series • Bind-IT™PDS 735.pdfMSDS ProMag Bind-IT.pdf
PMC1NProMag™ 1 Series • COOH PDS 715.pdfMSDS ProMag COOH-NH2.pdf
PMS1NProMag™ 1 Series • StreptavidinPDS 715.pdfMSDS ProMag SA-GAM.pdf
PMA3NProMag™ 3 Series • AminePDS 715.pdf MSDS ProMag COOH-NH2.pdf
PMB3NProMag™ 3 Series • Bind-IT™PDS 735.pdfMSDS ProMag Bind-IT.pdf
PMC3HPProMag™ HP 3 Series • COOHPDS 743.pdfMSDS ProMag COOH-NH2.pdf
PMC3NProMag™ 3 Series • COOHPDS 715.pdfMSDS ProMag COOH-NH2.pdf
PMM3NProMag™ 3 Series • Goat anti-Mouse IgG (Fc)PDS 723.pdfMSDS ProMag SA-GAM.pdf
PMG3NProMag™ 3 Series • Protein GPDS 715.pdfMSDS ProMag Protein G
PMS3HPProMag™ HP 3 Series • StreptavidinPDS 743.pdfMSDS ProMag SA-GAM.pdf
PMS3NProMag™ 3 Series • StreptavidinPDS 715.pdfMSDS ProMag SA-GAM.pdf

COMPEL™

COMPEL™ are highly uniform superparamagnetic microspheres ideal for applications that demand uniform particle response, such as miniaturized bioassays and separations. COMPEL™ are comprised of iron oxide crystals dispersed in a polymer matrix, with a functional polymer overcoating for the encapsulation of magnetite and the introduction of reactive groups. As they are polymer-based, they retain the low density that is advantageous for biomolecular assays. Our initial offerings include 3µm, 6µm, and 8µm beads with measured CVs of ≤ 5% and high levels of COOH-functionalization. Customers ordering our 0.25g offering will also receive a 1.5mL magnetic separator - absolutely free!

Catalog Code DescriptionDiameter View Histograms Product Data Sheet
UMC3NCOMPEL™ Magnetic COOH modified3µm, 6µm3µm, 6µmPDS 705.pdf
UMC4NCOMPEL™ Magnetic COOH modified8µm8µmPDS 705.pdf
UMC3FCOMPEL™ Magnetic COOH modified, fluorescent3µm, 6µm3µm, 6µmPDS 705.pdf
UMC4FCOMPEL™ Magnetic COOH modified, fluorescent8µm8µmPDS 705.pdf
CM01NCOMPEL™ Magnetic Streptavidin modified
3µm, 6µm3µm, 6µmPDS 721.pdf
CM01NCOMPEL™ Magnetic Streptavidin modified8µm8µmPDS 721.pdf

Fluorescent Magnetic Polymers

Both Classical (MC catalog codes) and Encapsulated (ME catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the bead matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

Catalog Code DescriptionDiameter Product Data Sheet
MC03FClassical Magnetic Polymer0.50 - 0.99PDS 731.pdf
ME03FEncapsulated Magnetic Polymer0.50 - 0.99PDS 731.pdf
ME04FEncapsulated Magnetic Polymer1.00 - 1.99PDS 731.pdf
UMC3FCOMPEL™ Magnetic COOH modified, fluorescent3µm, 6µmPDS 705.pdf
UMC4FCOMPEL™ Magnetic COOH modified, fluorescent8µmPDS 705.pdf

BioMag® and BioMag®Plus

BioMag® Superparamagnetic Particles are high-performance superparamagnetic microparticles widely used in the magnetic separation of cells, organelles, proteins, immunoglobulins, nucleic acids, and many other types of molecules in biological and non-biological systems. Their irregular shape affords a much greater surface area than that of the same size spherical particle. This large surface area results in high binding capacities, allowing for efficient capture of target with minimum amounts of particles. BioMag® particles are greater than 90% iron oxide resulting in a fast magnetic separation, especially on high throughput automated platforms. The particles are covered with a coating that provides functional groups for the attachment of proteins or antibodies.

There are three varieties of BioMag® Superparamagnetic Microparticles: BioMag®, BioMag®Plus, and BioMag® Maxi. BioMag® particles have a mean diameter of ~1.5µm (with the exception of BioMag® Superparamagnetic Iron Oxide [~10µm]). BioMag®Plus particles are similar to conventional BioMag® particles with the important distinction of fewer fines. Additionally, BioMag®Plus particles are often offered in kits as the principle component. BioMag® Maxi particles have a mean diameter of ~3-12µm.

BioMag® and BioMag®Plus Unconjugated Particles

BioMag® and BioMag®Plus Unconjugated Particles are offered for the covalent attachment of proteins or ligands while allowing for the retention of biological activity.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM546BioMag® Amine5010
100
PDS 546.pdfMSDS BM546.pdf
BP617BioMag®Plus Amine5010
100
PDS 617.pdfMSDS BP617.pdf
BP610BioMag®Plus Amine
Protein Coupling Kit
5025PDS 617.pdfMSDS BP617.pdf
MSDS Glutaraldehyde.pdf
MSDS Pyridine Wash Buffer.pdf
MSDS Quenching Solution.pdf
MSDS Wash Buffer.pdf
BM570BioMag® Carboxyl2010
100
PDS 570.pdf
PDS 725.pdf
MSDS BM570.pdf
BP618BioMag®Plus Carboxyl2010PDS 618.pdf
PDS 725.pdf
MSDS BP618.pdf
BP611BioMag®Plus Carboxyl
Protein Coupling Kit
202.5PDS 618.pdf
PDS 725.pdf
MSDS BP618.pdf
MSDS EDC.pdf
MSDS MES Buffer.pdf
MSDS Quenching Solution.pdf
MSDS Wash Buffer.pdf

BioMag® Magnetic Immobilization Kit

The BioMag® Magnetic Immobilization Kit is designed to introduce the user to the ease of magnetizing biological molecules. The kit includes BioMag® Amine particles, glutaraldehyde (reagent used to couple proteins to BioMag® particles), a BioMag® Flask Separator, and a Reaction Flask.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM545BioMag® Magnetic Immobilization Kit5010PDS 546.pdf

MSDS BM546.pdf
MSDS Glutaraldehyde.pdf

BioMag® mRNA Purification

Nuclease-free suspensions of BioMag® with covalently-bound oligo dT (20) are utilized for the efficient isolation of polyadenylated mRNA from total RNA, tissue, or cell lysate. Following isolation, mRNA is eluted through a simple incubation step in DEPC-treated water. BioMag® Oligo dT (20) particles may be purchased alone or as a component of our BioMag® mRNA Purification System.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Number of
Reactions
Product Data Sheet
BM569BioMag® mRNA
Purification System
5220 x 100µg total RNA; or
10 x 50-100mg tissue; or
10 x lysate from 5 x 106 cells
PDS 569.pdf
BM529BioMag® Oligo dT (20),
Nuclease-free
5220 x 100µg total RNAPDS 529.pdf

BioMag® Streptavidin, Nuclease-free

Streptavidin-coated BioMag® particles are offered in a nuclease-free preparation for the binding or capture of biotinylated nucleic acids.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM568BioMag® Streptavidin, Nuclease-free110
25
PDS 530.pdfMSDS BM568.pdf

BioMag® and BioMag®Plus Binding Proteins

BioMag® particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule of interest through a straightforward incubation. They are also suitable for off-the-shelf use in applications, such as immunoprecipitation or PCR product clean-up.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM552BioMag® Biotin55PDS 552.pdfMSDS BM552.pdf
BM554BioMag® Protein A5
2
10
PDS 554.pdfMSDS BM554.pdf
BP620BioMag®Plus Protein A52
10
PDS 620.pdfMSDS BP620.pdf
BP614BioMag®Plus Protein A
Antibody Isolation Kit
52.5PDS 620.pdfMSDS BP620.pdf
MSDS Elution Buffer.pdf
MSDS Neutralization Buffer.pdf
MSDS Wash-Binding Buffer.pdf
BM553BioMag® Protein G52
10
PDS 553.pdfMSDS BM553.pdf
BP627BioMag®Plus Protein G52
10
PDS 620.pdfMSDS BP627.pdf
BP626BioMag®Plus Protein G
Antibody Isolation Kit
52.5PDS 620.pdfMSDS BP627.pdf
MSDS Elution Buffer.pdf
MSDS Neutralization Buffer.pdf
MSDS Wash-Binding Buffer.pdf
BM551BioMag® Streptavidin55
50
PDS 551.pdfMSDS BM551.pdf
BP628BioMag®Plus Streptavidin510PDS 621.pdfMSDS BP628.pdf
BM568BioMag® Streptavidin,
Nuclease-free
1
10
25
PDS 530.pdfMSDS BM568.pdf
BP621BioMag®Plus Streptavidin /
Biotin Binding Kit
55PDS 621.pdfMSDS BP628.pdf
MSDS Wash-Coupling Solution.pdf
BP530BioMag®Plus Wheat Germ
Agglutinin
53
10
PDS 716.pdfMSDS BP530.pdf
BP531BioMag®Plus Concanavalin A53
10
PDS 720.pdfMSDS BP531.pdf

BioMag® and BioMag®Plus Protein Isolation and Removal Kits

With their tremendous surface area, BioMag® particles are ideal for purifications. Our antibody isolation kits provide a means for the efficient isolation of antibody from serum or cell culture supernatant, and our protein removal kits permit the depletion of ubiquitous proteins, such as albumin and immunoglobulin from serum, for a significant improvement in resolution of less abundant proteins.

Catalog Code Description Number of Reactions Product Data SheetMaterial Safety Data Sheet
BP614BioMag®Plus Protein A
Antibody Isolation Kit
5 x 5µL serum or
cell culture supernatant
PDS 620.pdfMSDS BP620.pdf
MSDS Elution Buffer.pdf
MSDS Neutralization Buffer.pdf
MSDS Wash-Binding Buffer.pdf
BP626BioMag®Plus Protein G
Antibody Isolation Kit
5 x 5µL serum or
cell culture supernatant
PDS 620.pdfMSDS BP627.pdf
MSDS Elution Buffer.pdf
MSDS Neutralization Buffer.pdf
MSDS Wash-Binding Buffer.pdf
BP658BioMag® ProMax Albumin
Removal Kit
25 x 10µL serum
PDS 658.pdfMSDS BP658.pdf
MSDS Binding-Wash Buffer.pdf
MSDS Elution Buffer.pdf
BP659BioMag® ProMax Serum IgG
Removal Kit
10 x 1µL serum
PDS 659.pdfMSDS BP659.pdf
MSDS Binding-Wash Buffer.pdf

BioMag® and BioMag®Plus Secondary Antibodies

BioMag® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles.

Catalog CodeDescriptionConcentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM563BioMag® Goat anti-Human IgG150PDS 563.pdfMSDS BM563.pdf
BM562BioMag® Goat anti-Human IgG
(Fc Specific)
550PDS 562.pdfMSDS BM562.pdf
BM561BioMag® Goat anti-Human IgM1
50PDS 561.pdfMSDS BM561.pdf
BM549BioMag® Goat anti-Mouse IgG150
500
PDS 549.pdfMSDS BM549.pdf
BM550BioMag® Goat anti-Mouse IgG
(Fc Specific)
550
500
PDS 550.pdfMSDS BM550.pdf
BP619BioMag®Plus Goat anti-Mouse IgG150PDS 619.pdfMSDS BP619.pdf
BP612BioMag®Plus Goat anti-Mouse
IgG Antibody Coupling Kit
125PDS 619.pdfMSDS BP619.pdf
MSDS Wash-Coupling Solution.pdf
BM558BioMag® Goat anti-Mouse IgM150
500
PDS 558.pdfMSDS BM558.pdf
BM559BioMag® Goat anti-Rabbit IgG150
500
PDS 559.pdfMSDS BM559.pdf
BM560BioMag® Goat anti-Rat IgG150
500
PDS 560.pdfMSDS BM560.pdf
BM548BioMag® Goat anti-Rat IgG
(Fc Specific)
550
500
PDS 548.pdfMSDS BM548.pdf
BM557BioMag® Goat anti-Rat IgM550
500
PDS 557.pdfMSDS BM557.pdf

BioMag®Plus Mouse anti-Fluorescein IgG

BioMag®Plus Mouse anti-Fluorescein IgG particles may be used to isolate fluorescein-tagged species, such as cells or nucleic acids.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BP622BioMag®Plus Mouse
anti-Fluorescein IgG
150PDS 622.pdfMSDS BP622.pdf

BioMag® anti-Leukocyte for Human

BioMag® anti-Leukocyte particles are coated with anti-CD antibodies for the positive selection of specific cell subsets from purified leukocyte preps or whole blood. Positive selections permit rapid and efficient isolation of target cell populations.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM595BioMag® anti-Human CD245PDS 595.pdfMSDS BM595.pdf
BM580BioMag® anti-Human CD345PDS 580.pdfMSDS BM580.pdf
BM581BioMag® anti-Human CD41.55PDS 581.pdfMSDS BM581.pdf
BM583BioMag® anti-Human CD81.55PDS 583.pdfMSDS BM583.pdf
BM596BioMag® anti-Human CD11b15PDS 596.pdfMSDS BM596.pdf
BM584BioMag® anti-Human CD141.55PDS 584.pdfMSDS BM584.pdf
BM585BioMag® anti-Human CD1645PDS 585.pdfMSDS BM585.pdf
BM586BioMag® anti-Human CD1945PDS 586.pdfMSDS BM586.pdf
BM587BioMag® anti-Human CD3445PDS 587.pdfMSDS BM587.pdf
BM588BioMag® anti-Human CD4545PDS 588.pdfMSDS BM588.pdf
BM589BioMag® anti-Human CD5645PDS 589.pdfMSDS BM589.pdf
BM590BioMag® anti-Human CD7145PDS 590.pdfMSDS BM590.pdf

BioMag® anti-Leukocyte for Mouse

BioMag® anti-Leukocyte particles are coated with anti-CD antibodies for the positive selection of specific cell subsets from purified leukocyte preps or whole blood. Positive selections permit rapid and efficient isolation of target cell populations.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM592BioMag® anti-Mouse CD415PDS 592.pdfMSDS BM592.pdf
BM593BioMag® anti-Mouse CD8a15PDS 593.pdfMSDS BM593.pdf
BM594BioMag® anti-Mouse CD45R15PDS 594.pdfMSDS BM594.pdf

BioMag® Human T cell Enrichment Systems

BioMag® Human T cell Enrichment Systems are available for the enrichment of CD3+, CD4+, or CD8+ T cell fractions from human peripheral blood mononuclear cells. Our enrichment systems involve the magnetic removal of subpopulations positive for other CD markers, leaving the untouched CD3+, CD4+, or CD8+ fraction available for analysis.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM597BioMag® Human CD3+ T cell
Enrichment System
11
5
PDS 597.pdfMSDS BM597-BM599.pdf
BM598BioMag® Human CD4+ T cell
Enrichment System
11
5
PDS 597.pdfMSDS BM597-BM599.pdf
BM599BioMag® Human CD8+ T cell
Enrichment System
11
5
PDS 597.pdfMSDS BM597-BM599.pdf

BioMag® Dextran-coated Charcoal

BioMag® Dextran-coated Charcoal is suitable for the adsorption of free tritium-labeled analyte in typical competitive radioimmunoassays. BioMag® particles are coated with NORIT® activated carbon and dextran.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM555BioMag® Dextran-coated Charcoal5100PDS 555.pdfMSDS BM555.pdf
BM556BioMag® Dextran-coated Charcoal Concentrate401000PDS 555.pdfMSDS BM556.pdf

BioMag® Superparamagnetic Iron Oxide

BioMag® Superparamagnetic Iron Oxide may be used for applications in which an iron oxide particle is required. Particles are ~10µm irregularly-shaped iron oxide clusters, and are non-functionalized.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BM547BioMag® Superparamagnetic Iron Oxide (~10µm)5010PDS 547.pdfMSDS BM547.pdf

BioMag® Maxi

BioMag® Maxi have the same structure and composition as standard BioMag® products (silanized iron oxide clusters), but with a larger mean diameter of ~3-12µm.

Catalog Code Description Concentration
(mg/mL)
Volume
(mL)
Product Data SheetMaterial Safety Data Sheet
BMM30BioMag® Maxi Carboxyl2010PDS 630.pdfMSDS BMM30.pdf
BMM40BioMag® Maxi Amine5010PDS 640.pdfMSDS BMM40.pdf

QuantumPlex™M

QuantumPlex™M (Magnetic) kits consist of five populations of ~6µm highly uniform superparamagnetic microspheres encoded with different intensities of Starfire Red™. Like QuantumPlex™, they are suitable for use on standard flow cytometers. Highly efficient separations may be performed using rare earth magnetic separators. Carboxyl (COOH) and streptavidin surfaces are available. Carboxyl populations are at a concentration of ~1x108 beads/mL, while Streptavidin populations are at a concentration of ~1x106 beads/mL. QuantumPlex™M are available in three sizes: A (1mL), B (5mL), and C (10mL).

QuantumPlex™M SP (Magnetic, Single Population) is useful for the development of simplex flow cytometric assays, or for the optimization of attachment chemistry and assay parameters before transitioning to a multiplexed format. Carboxyl (COOH) and streptavidin surfaces are available. Carboxyl populations are at a concentration of ~1x108 beads/mL, while Streptavidin and anti-Mouse IgG populations are at a concentration of ~1x106 beads/mL. QuantumPlex™M SP are available in two sizes: A (1mL) or  B (3mL).

Catalog Code Description Product Data Sheet Material Safety Data Sheet
250QuantumPlex™M COOH, 5 populations
PDS 250.pdfMSDS 250.pdf
252QuantumPlex™M Streptavidin, 5 populations
PDS 252.pdfMSDS 252.pdf
251QuantumPlex™M SP COOHPDS 251.pdfMSDS 251.pdf
253QuantumPlex™M SP StreptavidinPDS 253.pdfMSDS 253.pdf