We manufacture a number of standards that are intended for use with either image-based cell viability analyzers (e.g. trypan blue, Vi-CELL®) or flow cytometers (DAPI, propidium iodide, LIVE/DEAD® stains, etc) that are important to bioprocessing and research programs.

ViaCheck™ Viability and Concentration Standards

Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Viability Controls are supplied at ~1.0 x 106 beads / mL and are available in a range of live/dead ratios. ViaCheck™ Concentration Controls are supplied at the noted concentrations. For information on proper mixing and sampling, see TSD 0706: Handling & Pipetting Concentration Standards. or to request a copy of Optimization of Vi-CELL® XR Settings for Calibration Checks Using ViaCheck™ Controls & ViaCheck™ for Cell Viability Analyzers: Best Practices, click here.

Catalog Number Description Volume (mL) Product Data Sheet Safety Data Sheet
VC10B ViaCheck™ 0% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC25B ViaCheck™ 25% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC20B ViaCheck™ 50% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC30B ViaCheck™ 75% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC40B ViaCheck™ 90% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC50B ViaCheck™ 100% Viability Control 20 PDS 706.pdf SDS ST352.pdf
VC60N ViaCheck™ Concentration Control (1 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC70N ViaCheck™ % Concentration Control (4 × 106) 20 PDS 711.pdf SDS ST353.pdf
VC80N ViaCheck™ % Concentration Control (8 × 106) 20 PDS 711.pdf SDS ST353.pdf

 ViaCheck™ Viability and Concentration Standards - SingleShots™

ViaCheck™ SingleShots™, affordable single-use QC. Mix, dispense & run for confirmation of live/dead ratios and counts. ViaCheck™ SingleShots™ minimize error and maximize efficiency by circumventing potential human error from repeated bottle resuspension & pipetting steps. To request a copy of Optimization of Vi-CELL® XR Settings for Calibration Checks Using ViaCheck™ Controls & ViaCheck™ for Cell Viability Analyzers: Best Practices, click here.

Catalog Number Description Units Product Data Sheet Safety Data Sheet
VC10BSS-25 ViaCheck™ 0% Viability Control SingleShots™ 25 PDS 706.pdf SDS ST352.pdf
VC10BSS-75 ViaCheck™ 0% Viability Control SingleShots™ 75 PDS 706.pdf SDS ST352.pdf
VC25BSS-25 ViaCheck™ 25% Viability Control SingleShots™ 25 PDS 706.pdf SDS ST352.pdf
VC25BSS-75 ViaCheck™ 25% Viability Control SingleShots™ 75 PDS 706.pdf SDS ST352.pdf
VC20BSS-25 ViaCheck™ 50% Viability Control SingleShots™ 25 PDS 706.pdf SDS ST352.pdf
VC20BSS-75 ViaCheck™ 50% Viability Control SingleShots™ 75 PDS 706.pdf SDS ST352.pdf
VC30BSS-25 ViaCheck™ 75% Viability Control SingleShots™ 25 PDS 706.pdf SDS ST352.pdf
VC30BSS-75 ViaCheck™ 75% Viability Control SingleShots™ 75 PDS 706.pdf SDS ST352.pdf
VC40BSS-25 ViaCheck™ 90% Viability Control SingleShots™ 25 PDS 706.pdf SDS ST352.pdf
VC40BSS-75 ViaCheck™ 90% Viability Control SingleShots™ 75 PDS 706.pdf SDS ST352.pdf
VC50BSS-25 ViaCheck™ 100% Viability Control SingleShots™ 25 PDS 706.pdf SDS ST352.pdf
VC50BSS-75 ViaCheck™ 100% Viability Control SingleShots™ 75 PDS 706.pdf SDS ST352.pdf
VC60NSS-25 ViaCheck™ Concentration Control (1 × 106) SingleShots™ 25 PDS 711.pdf SDS ST353.pdf
VC60NSS-75 ViaCheck™ Concentration Control (1 × 106) SingleShots™ 75 PDS 711.pdf SDS ST353.pdf
VC70NSS-25 ViaCheck™ % Concentration Control (4 × 106) SingleShots™ 25 PDS 711.pdf SDS ST353.pdf
VC70NSS-75 ViaCheck™ % Concentration Control (4 × 106) SingleShots™ 75 PDS 711.pdf SDS ST353.pdf
VC80NSS-25 ViaCheck™ % Concentration Control (8 × 106) SingleShots™ 25 PDS 711.pdf SDS ST353.pdf
VC80NSS-75 ViaCheck™ % Concentration Control (8 × 106) SingleShots™ 75 PDS 711.pdf SDS ST353.pdf

References

Kaminski RW, Wu M, Turbyfill  KR, Clarkson K, Tai B, Bourgeois AL, Van De Verg LL, Walker RI, Oaks EV. (2014) Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine. Clin Vaccine Immunol; 21(3):366-382.

Cadena-Herrera D, Esparza-De Lara JE, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E. (2015) Validation of three viable-cell counting methods: manual, semi-automated, and automated. Biotechnol Reports; 7:9-16.

Lew C, Gomez J, Rhyner M (2012) Instrument-to-instrument Variability in the Vi-CELL Automated Viability Analyzer. Beckman Coulter Life Sciences

Fluorescence Reference Standards - FLOW

We offer microspheres labeled with traditional dyes/stains that may be used as standards to support flow cytometric assays in cell cycle analysis or cell viability determinations. Beads may be used for routine instrument set-up and QC, or as test-specific standards. For additional information regarding cell cycle analysis, see our Cell Cycle Analysis brochure.

Catalog Number Description Product Data Sheet Material Safety Data Sheet
897 Acridine Orange PDS 890.pdf SDS ST334.pdf
906 DAPI PDS 890.pdf SDS ST334.pdf
891 Fluorescein (FITC) PDS 890.pdf SDS ST331.pdf
894 Hoechst 33342 PDS 890.pdf SDS ST334.pdf
892 Propidium Iodide PDS 890.pdf SDS ST334.pdf

Viability Dye Compensation Standard - Flow

Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain.

Catalog Number Description Product Data Sheet Safety Data Sheet
450 Viability Dye Compensation Standard, 4µm PDS 853.pdf SDS ST340.pdf
451 Viability Dye Compensation Standard, 8µm PDS 853.pdf SDS ST340.pdf

See also our range of Fluorescence Reference Standards for beads labeled with common viability dyes such as DAPI, Propidium Iodide, etc.