FITC / PE Compensation Standard

The FITC / PE Compensation Standard includes four microsphere populations that are ~7-9µm in diameter: an Autofluor™ population, and single populations surface-labeled with FITC, PE, and FITC / PE. The Autofluor™ population is dyed with a low level of fluorophore to approximate the autofluorescence of an unstained resting lymphocyte. This kit is useful for establishing quadrant boundaries and compensation settings for analyses using FITC and PE fluorochromes.

Catalog Number Description Product Data Sheet Safety Data Sheet
820 FITC / PE Compensation Standard (Autofluor™, FITC-PE, FITC/PE) PDS 820.pdf SDS ST334.pdf

Flow Cytometry Antibody Binding Beads

Single population Protein A or Protein G microspheres are suitable for labeling with conjugated antibodies from a range of hosts. Labeled microspheres may be used as single-population reference standards or in conjunction with an unlabeled population for compensation purposes.
 

Catalog Number Description Product Data Sheet Safety Data Sheet
553 Flow Cytometry Protein A Antibody Binding Beads PDS 854.pdf SDS ST338.pdf
554 Flow Cytometry Protein G Antibody Binding Beads PDS 854.pdf SDS ST338.pdf

Simply Cellular® Compensation Standard

The Simply Cellular® Compensation Standard includes a mixed population of low- and high-binding antibody-capture beads that are ~7-9µm in diameter. Users label aliquots with fluorescent antibody conjugates of interest to establish suitable compensation settings for a specific analysis.

Catalog Number Description Product Data Sheet Safety Data Sheet
550 Simply Cellular® Compensation Standard (anti-Mouse IgG) PDS 850.pdf SDS ST337.pdf
556 Simply Cellular® Compensation Standard - High (anti-Mouse IgG) PDS 850.pdf SDS ST337.pdf
551 Simply Cellular® Compensation Standard (anti-Rat IgG) PDS 851.pdf SDS ST337.pdf
552 Simply Cellular® Compensation Standard (anti-Human IgG) PDS 852.pdf SDS ST337.pdf

References

Wang J-C E, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA. (2009) An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform. J Immunol Methods; 341:106-116.

Scheible K, Secor-Socha S, Wightman T, Wang H, Mariani T.J., Topham DJ,…Quataert S (2012) Stability of T Cell Phenotype and Functional Assays Following Heparinized Umbilical Cord Blood Collection. Cytometry A 81(11):937–949

Simply Cellular® anti-Mouse for Violet Laser

The Simply Cellular® anti-Mouse for Violet Laser standard features microspheres comprised of a proprietary matrix that exhibits low autofluorescence with violet excitation. Beads are suitable for labeling with mouse mAbs conjugated with violet fluorochromes, and for use as a compensation or general reference standard for detectors off of the violet laser. Beads are also suitable for use with other fluorochromes / detectors, e.g. 488nm, 633nm. The Simply Cellular® anti-Mouse for Violet Laser standard is supplied as 2 populations: 1 blank and 1 high-binding anti-Mouse IgG (Fc-specific) population, each are supplied in aqueous suspension containing ProClin®.

Catalog Number Description Product Data Sheet Safety Data Sheet
835 Simply Cellular® anti-Mouse for Violet Laser PDS 835.pdf SDS ST339.pdf

Viability Dye Compensation Standards

Viability Dye Compensation Standards are suitable for labeling with LIVE/DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain.

Catalog Number Description Product Data Sheet Safety Data Sheet
450 Viability Dye Compensation Standard, 4µm PDS 853.pdf SDS ST340.pdf
451 Viability Dye Compensation Standard, 8µm PDS 853.pdf SDS ST340.pdf

References

Perfetto SP, Chattopadhyay PK, Lamoreaux L, Nguyen R, Ambrozak D, Koup RA, Roederer M. (2006). Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. J Immunol Methods; 313(1-2):199-208.

Perfetto SP, Chattopadhyay PK, Lamoreaux L, Nguyen R, Amrozak D, Koup RA, Roederer M. (2010) Amine-reactive dyes for dead cell discrimination in fixed samples. Curr Protoco Cytom; Jul; CHAPTER: Unit-9.34. (SIMPLX)

Lamoreaux, L, Roederer, M, Koup, R (2206) Intracellular cytokine optimization and standard operating procedure. Nat. Protocols 1, 1507–1516

Lugli E, Gattinoni L, Roberto A, Mavilio D, Price DA, Restifo NR, Roederer M. (2012) Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells. Nature Protocol v8(1)

Fluorescence Reference

Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. They may be used to QC a specific path of the optical system, to optimize filter sets for fluorophores, to establish test-specific Target Channel values for instrument set-up, and as general compensation or intensity standards.

Catalog Number Description

Product Data Sheet

Safety Data Sheet
890 Certified Blank™ PDS 890.pdf SDS ST341.pdf
897 Acridine Orange PDS 890.pdf SDS ST334.pdf
886 Alexa Fluor® 488 PDS 890.pdf SDS ST334.pdf
887 Alexa Fluor® 647 PDS 890.pdf SDS ST332.pdf
901 Allophycocyanine (APC) PDS 890.pdf SDS ST334.pdf
914 APC-Cy™7 PDS 890.pdf SDS ST334.pdf
898 Chlorophyll PDS 890.pdf SDS ST334.pdf
895 Cy™5 PDS 890.pdf SDS ST334.pdf
906 DAPI PDS 890.pdf SDS ST334.pdf
913 Far-Out Red PDS 890.pdf SDS ST335.pdf
891 Fluorescein (FITC), PDS 890.pdf SDS ST331.pdf
894 Hoechst 33342 PDS 890.pdf SDS ST334.pdf
916 Pacific Blue™ PDS 890.pdf SDS ST333.pdf
908 PE-Cy™5 PDS 890.pdf SDS ST334.pdf
889 PE-Cy™7 PDS 890.pdf SDS ST334.pdf
909 PE-TR PDS 890.pdf SDS ST334.pdf
892 Propidium Iodide PDS 890.pdf SDS ST334.pdf
899 R-Phycoerythrin (PE) PDS 890.pdf SDS ST334.pdf
905 T.M. Rhodamine PDS 890.pdf SDS ST334.pdf
893 Texas Red® (TR) PDS 890.pdf SDS ST334.pdf
915 Violet Laser PDS 890.pdf SDS ST335.pdf