Bangs offers a broad portfolio of standards for color compensation. Our offerings include beads that may be labeled with the antibody conjugate or amine-reactive dye of choice, as well as reference standards pre-labeled with common flurophores.

FLOW CYTOMETRY ANTIBODY BINDING BEADS

Single population Protein A or Protein G microspheres are suitable for labeling with conjugated antibodies from a range of hosts. Labeled microspheres may be used as single-population reference standards or in conjunction with an unlabeled population for compensation purposes.



TSD 0025, Protein A & G Antibody Affinities
PDS 854, Flow Cytometry Protein A & Protein G Antibody Binding Beads
SDS ST338, (Protein A & G Binding Beads) 

Catalog Number Description
553 Flow Cytometry Protein A Antibody Binding Beads
554 Flow Cytometry Protein G Antibody Binding Beads

REFERENCES

Musil, J, Kutinova, L, Zurkova, K, Hainz, P, Babiarova, K, Krystofova, J, & Nemeckova, S. (2014) Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3. Cancer gene therapy, 21(3), 115.

 

Simply Cellular® Compensation Standard

The Simply Cellular® Compensation Standard includes a mixed population of low- and high-binding antibody-capture beads that are ~7-9µm in diameter. Users label aliquots with fluorescent antibody conjugates of interest to establish suitable compensation settings for a specific analysis.

PDS 850, Simply Cellular® anti-Mouse Compensation Standard & anti-Mouse Compensation Standard - High
PDS 851, Simply Cellular® anti-Rat Compensation Standard
PDS 852, Simply Cellular® anti-Human Compensation Standard
SDS ST337, (Simply Cellular® Compensation Standards)

Catalog Number Description
550 Simply Cellular® Compensation Standard (anti-Mouse IgG)
556 Simply Cellular® Compensation Standard - High (anti-Mouse IgG)
551 Simply Cellular® Compensation Standard (anti-Rat IgG)
552 Simply Cellular® Compensation Standard (anti-Human IgG)

References

Wang J-C E, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA. (2009) An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform. J Immunol Methods; 341:106-116.

Scheible K, Secor-Socha S, Wightman T, Wang H, Mariani T.J., Topham DJ,…Quataert S (2012) Stability of T Cell Phenotype and Functional Assays Following Heparinized Umbilical Cord Blood Collection. Cytometry A 81(11):937–949

Simply Cellular® anti-Mouse for Violet Laser

The Simply Cellular® anti-Mouse for Violet Laser standard features microspheres comprised of a proprietary matrix that exhibits low autofluorescence with violet excitation. Beads are suitable for labeling with mouse mAbs conjugated with violet fluorochromes, and for use as a compensation or general reference standard for detectors off of the violet laser. Beads are also suitable for use with other fluorochromes / detectors, e.g. 488nm, 633nm. The Simply Cellular® anti-Mouse for Violet Laser standard is supplied as 2 populations: 1 blank and 1 high-binding anti-Mouse IgG (Fc-specific) population, each are supplied in aqueous suspension containing ProClin®.

PDS 835, Simply Cellular® anti-Mouse for Violet Laser
SDS ST339, (Simply Cellular® anti-Mouse for Violet Laser)

Catalog Number Description
835 Simply Cellular® anti-Mouse for Violet Laser

Viability Dye Compensation Standards

Viability Dye Compensation Standards are suitable for labeling with LIVE/DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain.


PDS 853, Viability Dye Compensation Standard
SDS ST340, (Viability Dye Compensation Standard)

Catalog Number Description
450 Viability Dye Compensation Standard, 4µm
451 Viability Dye Compensation Standard, 8µm

References

Perfetto SP, Chattopadhyay PK, Lamoreaux L, Nguyen R, Ambrozak D, Koup RA, Roederer M. (2006). Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. J Immunol Methods; 313(1-2):199-208.

Perfetto SP, Chattopadhyay PK, Lamoreaux L, Nguyen R, Amrozak D, Koup RA, Roederer M. (2010) Amine-reactive dyes for dead cell discrimination in fixed samples. Curr Protoco Cytom; Jul; CHAPTER: Unit-9.34. (SIMPLX Amine active beads [Cat. 450, 451])., 

Lamoreaux, L, Roederer, M, Koup, R (2006) Intracellular cytokine optimization and standard operating procedure. Nat. Protocols 1, 1507–1516. (Amine-modified microspheres, PAO5N [Cat. 450, 451]).

Lugli E, Gattinoni L, Roberto A, Mavilio D, Price DA, Restifo NR, Roederer M. (2012) Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells. Nature Protocol v8(1).  (SMPLX R-NH2 Beads [Cat. 450, 451], stained for LIVE/DEAD Aqua Comp Beads)

Estes, M. L., Mund, J. A., Ingram, D. A., & Case, J. (2010). Identification of endothelial cells and progenitor cell subsets in human peripheral blood. Current protocols in cytometry, 9-33.(Cat. 450, 451 Viability Dye Compensation Standard [Amine Polymer Microspheres, PA04N] [Cat. 450, 451]).

FITC / PE COMPENSATION STANDARD

The FITC / PE Compensation Standard includes four microsphere populations that are ~7-9µm in diameter: an Autofluor™ population, and single populations surface-labeled with FITC, PE, and FITC / PE. The Autofluor™ population is dyed with a low level of fluorophore to approximate the autofluorescence of an unstained resting lymphocyte. This kit is useful for establishing quadrant boundaries and compensation settings for analyses using FITC and PE fluorochromes.


PDS 820, FITC / PE Compensation Standard
SDS ST334, (FITC / PE Compensation Standard)

Catalog Number Description
820 FITC / PE Compensation Standard (Autofluor™, FITC-PE, FITC/PE)

Fluorescence Reference

Single-color Fluorescence Reference Standards for flow cytometry are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. They may be used to QC a specific path of the optical system, to optimize filter sets for fluorophores, to establish test-specific Target Channel values for instrument set-up, and as general compensation or intensity standards.

Flow Cytometry Fluorescence Reference Chart
PDS 890, Fluorescence Reference Standards
SDS ST331, (FITC Reference Standard)
SDS ST332, (Alexa Fluor® 647 Reference Standard)
SDS ST333, (Pacific Blue™ Reference Standard)
SDS ST334, (General Fluorescence Reference Standard)
SDS ST335, (Violet Laser & Far-Out Red Reference Standard)
SDS ST341, (Certified Blank™ Reference Standard)

Catalog Number Description
890 Certified Blank™
897 Acridine Orange
886 Alexa Fluor® 488
887 Alexa Fluor® 647
901 Allophycocyanine (APC)
898 Chlorophyll
895 Cy™5
906 DAPI
913 Far-Out Red
891 Fluorescein (FITC),
894 Hoechst 33342
916 Pacific Blue™
908 PE-Cy™5
892 Propidium Iodide
899 R-Phycoerythrin (PE)
905 T.M. Rhodamine
893 Texas Red® (TR)
915 Violet Laser